Studies on Polymyxin: An Agar Diffusion Method of Assay

Stansly, P. G.; Schlosser, M. E.

doi:10.1128/jb.54.5.585-597.1947

Cited by 31 publications

(8 citation statements)

References 3 publications

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“…Homologous patterns were observed when the same techniques were applied to plates seeded with Escherichia coli and exposed to polymyxin3 according to the method of Stansly and Schlosser (1947). E. coli under the influence of polymyxin and treated as above yields an even more spectacular response than does S. aureus exposed to penicillin and similarly treated.…”

Section: Experiments and Resultsmentioning

confidence: 95%

Cytochemical Mechanisms of Penicillin Action Viii

Pratt

Dufrénoy

1949

J Bacteriol

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Section: Experiments and Resultsmentioning

confidence: 95%

Cytochemical Mechanisms of Penicillin Action Viii

Pratt

Dufrénoy

1949

J Bacteriol

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“…In all instances, experimental flasks were in triplicate and an average potency was obtained from individual assays of the centrifuged fermentation liquor of each flask. Activity was determined either by the agar streak, serial dilution method (Waksman and Reilly, 1945) using Escherichia-coli (MacLeod strain), and expressed as a dilution, or by the more accurate agar diffusion method (Stansly and Schlosser, 1947) and expressed as units per ml. An assay titer of 1:128 is approximately equivalent to 128 units per ml.…”

Section: Methodsmentioning

confidence: 99%

Studies on Polymyxin: The Production of Fermentation Liquor

Stansly¹,

Schlosser²,

Ananenko³

et al. 1948

J Bacteriol

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“…The fact that a concentration series of each chemical agent was tested assures the certainty of the inhibiting level. The antibiotic, polymyxin-B-sulfate, best fitted the qualification of the desired selective inhibitor (Ainsworth et al, 1947;Benedict and Langlykke, 1947;Stansly et al, 1947) . An examination of its effect on Chlorella vulgaris revealed that this organism is as resistant as Scenedesmus, being unaffected by 1000 p.p.m.…”

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The Differential Action of Chemical Agents, Especially Polymyxin-B, on Certain Algae, Bacteria and Fungi

Galloway

Krauss

1959

American Journal of Botany

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Galloway, R. A., and R. W. Krauss (U. Maryland, College Park.) The differential action of chemical agents, especially polymyxin‐B, on certain algae, bacteria and fungi. Amer. Jour. Bot. 46(1): 40‐49. Illus. 1959.—Representatives from each of the major groups of compounds exhibiting toxicity toward microorganisms were screened to determine their relative effect on 2 green algae, Scenedesmus obliquas and Chlorella pyrenoidosa, 1 blue green alga, Anabaena variabilis, 4 species of bacteria in the genera Flavobacterium, Achromobacter, and Pseudomonas, and the fungus Aspergillus sydowii. The objective was the determination of the differential toxicity shown by chemical agents against these microorganisms. Polymyxin‐B sulfate proved to have a high degree of selectivity which warranted detailed examination of its action. Manometric studies were made of the effects of this compound on rates of photosynthesis and respiration. The rate of photosynthesis in Scenedesmus was only slightly diminished by 640 p.p.m. polymyxin‐B, but a rapid reduction in rate to a very low level was observed in Chlorella. The endogenous respiration rate in Scenedesmus was more than doubled. The rate of respiration on glucose, pyruvate, fumarate, and acetate remained unchanged except for a slight transient rise at a low pH or in the absence of magnesium. The effect of 640 p.p.m. of polymyxin‐B on photosynthesis of Chlorella and on respiration, whether endogenous or on any of the substrates mentioned above, was a rapid, permanent reduction to a low level. Similar reduction in rate of respiration at 160 p.p.m. was observed in the bacteria and fungus, when these organisms were utilizing lactose, pyruvate, or fumarate as substrates. Two of the bacteria were exceptions in that no reduction occurred when they were respiring lactose. Inhibition of glucose phosphorylation was not found in the algae. Therefore some system other than an alternative phosphorylation must account for resistance to polymyxin‐B.

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Studies on Polymyxin: An Agar Diffusion Method of Assay

Cited by 31 publications

References 3 publications

Cytochemical Mechanisms of Penicillin Action Viii

Cytochemical Mechanisms of Penicillin Action Viii

Studies on Polymyxin: The Production of Fermentation Liquor

The Differential Action of Chemical Agents, Especially Polymyxin-B, on Certain Algae, Bacteria and Fungi

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