Studies on Polyphenols Isolated from Branches of Prunus spinosa L. Species

Abstract

The aim of this study was to investigate the crude extracts isolated from branches of Prunus spinosa L. (Rosaceae), in order to quantify polyphenols and tannins using instrumental methods (UV-Vis spectrometry and HPTLC densitometry). The qualitative analysis on biomass revealed the presence of valuable compounds such as polyphenols, flavonoids and tannins. HPTLC fingerprinting of crude methanol extract showed several peaks, with different Rf values, corresponding to phytocompounds such as chlorogenic acid (Rf 0.52), neochlorogenic acid (Rf 0.58), caffeic acid (Rf 0.95) and protocatechuic acid (Rf 0.96). The quantitative analysis revealed that the branches of P. spinosa contain polyphenols (2.97�0.059%g gallic acid equivalent/g dried vegetal material) and tannins (0.90�0.033%g tannic acid equivalent/g dried vegetal material). The HPTLC densitometry analysis offers information about the amount of neochlorogenic acid in the vegetal material 0.12% g/g. The content in polyphenols and tannins suggests the possibilities to use this species to separate compounds with antioxidant potential. These results open perspectives for complex valorization of Prunus spinosa species, a bioresource known only for phytotherapeutical and nutritional potential of their fruits.

An Overview of the Phytochemical Composition of Different Organs of Prunus spinosa L., Their Health Benefits and Application in Food Industry

“…The study conducted by Ciuperca et al in 2019 [69] focused on extracting polyphenols from the branches of P. spinosa L. in Bacău County, Romania. The study found a polyphenol content of 2.97 ± 0.059 mg gallic acid equivalent/g of dry weight, as well as a tannin content of 0.90 ± 0.033 g tannic acid equivalent/g of dry weight.…”

Microwave-Assisted Extraction of Condensed Tannins from Branches of Prunus spinosa L.: Response Surface Modeling and Optimization

“…It is a dense and spiny shrub growing to 1-2 m on the fringe of forests, clearings and pastures. Blackthorn is an abundant source of bioactive polyphenols, such as phenolic acids (chlorogenic acid, neochlorogenic acid, caffeic acid and protocatechuic acid), flavonoids, anthocyanins, as well as condensed tannins [25][26][27][28] with proved antioxidant effects [29][30][31].…”

Kinetic Study on Solid-Liquid Extraction of Condensed Tannins from Rosa Canina Branches and Stems

“…The solvent moves into the vegetal matrix and solubilize the compounds with similar polarity. There are well known several conventional extraction techniques as well as some nonconventional, developed more recently [14][15][16]. The conventional techniques (solid-liquid extraction at room temperature or using heat reflux) use organic solvents (hexane, acetone, methanol, ethanol etc.)…”