Studies on Polyphosphoinositides in Developing Rat Brain

Uma, S.; Ramakrishnan, C. V.

doi:10.1111/j.1471-4159.1983.tb08073.x

Cited by 36 publications

(13 citation statements)

References 15 publications

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“…Among them, contribution by PA is a likely source. A rapid degradation of poly-PI after decapitation is known to occur in rats [Uma and Ramakrishnan, 1983;Huang and Sun, 19861. Thus, these results are in agreement with the contention that one of the initial biochemical changes occurring during ischemia is the stimulated breakdown of poly-PI by phospholipase C. This change is accompanied by massive Ca2+ influx and depolarization of the neuronal membranes.…”

Section: Discussionmentioning

confidence: 99%

Acidic phospholipids, diacylglycerols, and free fatty acids in gerbil brain: A comparison of ischemic changes resulting from carotid ligation and decapitation

Huang

Sun

1987

J of Neuroscience Research

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The levels of brain acidic phospholipids (poly-PI, PI, PA, and PS), DG, and FFA and their acyl group profiles were determined after induction of ischemia in gerbils by ligation of the common carotid arteries and decapitation. Ischemia induced by both procedures resulted in a significant decrease in poly-PI (20% for 1-min decapitation and 1-min ligation). Except for a 16% increase in PI in the 5-min decapitation group, no apparent change was found in other phospholipids after either ischemic condition. The level of DG was increased one- and three-fold after 1 and 5 min, respectively, of decapitative ischemic treatment. Ligation of the carotid arteries for 1 min resulted also in a one-fold increase in the DG level. The decapitative model resulted in a one- and five-fold increase in FFA level (with respect to 1 and 5 min, respectively), whereas ligation for 1 min resulted in an increase of 42% of the FFA. The acyl groups of poly-PI and PI in the control gerbil brain are enriched in 18:0 and 20:4, but those of DG, FFA, and PA have a higher proportion of 16:0 besides 18:0 and 20:4. However, a preferential increase in the proportion of 18:0 and 20:4 was shown for the DG and FFA in both types of ischemic treatments. It is concluded from the results that both models of ischemic treatment elicit a similar decrease in poly-PI and increase in DG, but differ in the amount of FFA release. The higher level of FFA release in the decapitative model suggests that other biochemical mechanisms may be activated to cause the additional release.

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Section: Discussionmentioning

confidence: 99%

Acidic phospholipids, diacylglycerols, and free fatty acids in gerbil brain: A comparison of ischemic changes resulting from carotid ligation and decapitation

Huang

Sun

1987

J of Neuroscience Research

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“…The heads of the rats were frozen immediately. Following the procedure of Uma and Ramakrishnan (1983), the brain was isolated. Liver, lung, heart, kidney and brain were collected in precooled petri dishes and were blotted free of blood and tissue fluid.…”

Section: Methodsmentioning

confidence: 99%

Protective effect of Spirulina on lead induced deleterious changes in the lipid peroxidation and endogenous antioxidants in rats

Upasani

Balaraman

2003

Phytotherapy Research

148

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The present study aims to investigate the protective effect of Spirulina on lead-induced changes in the levels of lipid peroxidation and endogenous antioxidants in liver, lung, heart, kidney and brain of rats. Levels of elemental lead were also measured in the organs of rats in all experimental groups. In the liver, lung, heart and kidney of lead-exposed animals, there was a significant (p < 0.001) increase in the lipid peroxidation and a decrease in the levels of endogenous antioxidants. Although, Spirulina did not affect the deposition of lead in organs apart from the brain, simultaneous administration of Spirulina to lead exposed animals significantly (p < 0.001) inhibited lipid peroxidation and restored the levels of endogenous antioxidants to normal. To conclude, Spirulina had a significant effect on scavenging free radicals, thereby protecting the organs from damage caused by the exposure to lead. Further more, Spirulina showed a significant (p < 0.05) decrease in the deposition of lead in the brain.

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“…Previous studies have shown that cell phosphoinositides exist in metabolically distinct and therefore functionally different pools in various tissues. In brain tissue, the rapid postmortem hydrolysis of a portion of the polyphosphoinositides suggests the presence of metabolically active and inert pools of these lipids (Sheltaway and Dawson, 1969;Hauser et al, 1971;Uma and Ramakrishnan, 1983). The formation of phosphoinositides in tissues also involves different metabolic routes.…”

mentioning

confidence: 99%

Manganese Stimulates the Incorporation of [³H]Inositol into a Pool of Phosphatidylinositol in Brain That Is Not Coupled to Agonist‐Induced Hydrolysis

Schoepp

1985

Journal of Neurochemistry

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Mn2+ greatly increases the incorporation of myo-[3H]inositol into phosphatidylinositol (PI) of brain and other tissues by stimulating the activity of a PI-myo-inositol exchange enzyme. This study examined the ability of norepinephrine (NE) and carbachol to stimulate the hydrolysis of [3H]PI formed in the absence and presence of Mn2+-stimulated [3H]inositol exchange. Rat cerebral cortical slices were incubated with myo-[3H]inositol for 60 min in an N-2-hydroxyethyl piperazine-N'-2-ethanesulfonic acid (HEPES) buffer without or with MnCl2 (1 mM). The tissue was washed and further incubated with unlabeled myo-inositol and LiCl (10 mM). Prelabeled slices were then incubated with NE (0.1 mM) or carbachol (1 mM) to induce agonist-stimulated [3H]PI hydrolysis. Mn2+ treatment resulted in eight- and sixfold increases in control levels of [3H]PI and [3H]inositol monophosphate [( 3H]IP), respectively. Both NE and carbachol stimulated [3H]IP formation in tissue prelabeled without or with manganese. However, the degree of stimulation (percentage of control values) was greatly attenuated in the presence of Mn2+. In the absence of Mn2+ treatment, NE decreased [3H]PI radioactivity in the tissue to 80% of control values. However, NE did not decrease [3H]PI radioactivity in the Mn2+-treated tissue. These data demonstrate that Mn2+ stimulates incorporation of myo-[3H]inositol into a pool of PI in brain that has a rapid turnover but is not coupled to agonist-induced hydrolysis.

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Studies on Polyphosphoinositides in Developing Rat Brain

Cited by 36 publications

References 15 publications

Acidic phospholipids, diacylglycerols, and free fatty acids in gerbil brain: A comparison of ischemic changes resulting from carotid ligation and decapitation

Acidic phospholipids, diacylglycerols, and free fatty acids in gerbil brain: A comparison of ischemic changes resulting from carotid ligation and decapitation

Protective effect of Spirulina on lead induced deleterious changes in the lipid peroxidation and endogenous antioxidants in rats

Manganese Stimulates the Incorporation of [³H]Inositol into a Pool of Phosphatidylinositol in Brain That Is Not Coupled to Agonist‐Induced Hydrolysis

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Studies on Polyphosphoinositides in Developing Rat Brain

Cited by 36 publications

References 15 publications

Acidic phospholipids, diacylglycerols, and free fatty acids in gerbil brain: A comparison of ischemic changes resulting from carotid ligation and decapitation

Acidic phospholipids, diacylglycerols, and free fatty acids in gerbil brain: A comparison of ischemic changes resulting from carotid ligation and decapitation

Protective effect of Spirulina on lead induced deleterious changes in the lipid peroxidation and endogenous antioxidants in rats

Manganese Stimulates the Incorporation of [3H]Inositol into a Pool of Phosphatidylinositol in Brain That Is Not Coupled to Agonist‐Induced Hydrolysis

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Manganese Stimulates the Incorporation of [³H]Inositol into a Pool of Phosphatidylinositol in Brain That Is Not Coupled to Agonist‐Induced Hydrolysis