Studies on properties of surfaces required for growth of mammalian cells in synthetic medium

Rappaport, Catherine

doi:10.1016/0014-4827(60)90118-x

Cited by 26 publications

(7 citation statements)

References 4 publications

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“…Cell detachment produces gross alterations in morphology (Leighton et al, 1973), whereas physical properties of unconventional growth surfaces including texture, total negative charge and proton exchange capacity all may influence cell morphology (Rappaport, 1960;Rappaport et al, 1960;Dalen, 1973;Folkman & Moscona, 1978). For these reasons, comprehensive studies on tissue cultures, employing the entire battery of available light and electron microscopy preparative techniques have been impossible.…”

mentioning

confidence: 98%

Intercellular Junctions In Fanft‐Induced Carcinomas Of Rat Urinary Bladder In Tissue Culture: In Situ Thin‐Section, Freeze‐Fracture, And Scanning Electron Microscopy Studies

Pauli¹,

Kuettner²,

Weinstein³

1979

Journal of Microscopy

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This paper describes a set of simple methods for comparative light and electron microscopy studies on tissue cultured tumour cells derived from both noninvasive and invasive carcinogen-induced rat urinary bladder carcinomas. Cells are grown on Thermanox plastic coverslips and fixed in situ. Each plastic coverslip is then divided with scissors into four parts: the first is processed for light microscopy, the second for thin-section electron microscopy, the third for freeze-fracture electron microscopy, and the fourth for scanning electron microscopy. In some experiments, portions of the culture which have first been examined by light microscopy are subsequently prepared for electron microscopy. In this way, the culture conditions are kept constant and comparison of structural features (i.e. intercellular junctions) by several preparative techniques is possible. Noninvasive and invasive rat bladder tumour cells, characterized by numerous pleomorphic microvilli, have normal zonulae occludentes at the apices of lateral surfaces of tumour cells in all cultures. In some areas of invasive tumour cells, occludens junctions are focally attenuated, consisting of only one or two strands, and occasionally the strands are discontinuous. Gap junctions, type PF-1, as well as numerous demosomes are present in all cell lines. Thus, intercellular junctions in noninvasive and invasive rate bladder epithelial cell lines bear a striking resemblance to those previously described in the comparable solid primary tumours. These culture systems may be useful for studying factors which influence the formation of intercellular junctions during malignant transformation.

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mentioning

confidence: 98%

Intercellular Junctions In Fanft‐Induced Carcinomas Of Rat Urinary Bladder In Tissue Culture: In Situ Thin‐Section, Freeze‐Fracture, And Scanning Electron Microscopy Studies

Pauli¹,

Kuettner²,

Weinstein³

1979

Journal of Microscopy

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“…Factors influencing the way in which granulosa cells attach to a surface may also be important. Cells are sensitive to the surface to which they attach (Rappaport et al, 1960), and to the medium in which they are immersed (Channing, Tsai & Sachs, 1976). In this study plating efficiencies were low compared with the proportion of cells excluding dye, and the conditions used for the preparation of cover slips and media may not have been optimal for the attachment of granulosa cells.…”

Section: Discussionmentioning

confidence: 84%

“…Because of doubts about the appropriateness of dye exclusion techniques for this purpose, an alternative procedure has been developed based on the fact that mammalian cells grow optimally only when attached to a surface (Rappaport, Poole & Rappaport, 1960). penicillin (50 i.u./ml) and streptomycin (50 ug/ml).…”

Section: Introductionmentioning

confidence: 99%

Estimation of viability of bovine granulosa cells

Metcalf¹

1982

Reproduction

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Granulosa cells harvested from non-atretic, antral follicles of cow (and pig and sheep) ovaries were incubated over glass cover slips in medium containing 20% (v/v) donor calf serum. Cell attachment to the cover slips was rapid, being in most cases complete within 3 h at 37 degrees C. There was little further change over the next 20 h. The number of bovine granulosa cells which attached to a cover slip was proportional to the volume of cell suspension added to the medium; and the amount of oestradiol secretion by attached cells in a testosterone-enriched medium increased in parallel with their number. Granulosa cells which did not attach within 3 h produced little oestradiol. There was no clear relationship between the number of nigrosin-impermeable cells in suspensions prepared from different follicles and plating efficiency. It is concluded that the 3-h attachment of granulosa cells in culture is a useful measure of the number of viable cells in a suspension and is to be preferred to less direct techniques based on dye exclusion.

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“…By means of alkali-treatment the negative charge could be varied, and cell attachnlent and growth were found to be optimal at different charge densities for different cell lines (Rappaport, Poole and Rappaport, 1960). Glass has not often been used in a true microcarrier system, because its high density requires stirring speeds that are not compatible with cell gro\vth.…”

Section: Ionic Microcllrriersmentioning

confidence: 99%

Microcarrier Cell Culture

Nilsson

1988

Biotechnology and Genetic Engineering Reviews

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Studies on properties of surfaces required for growth of mammalian cells in synthetic medium

Cited by 26 publications

References 4 publications

Intercellular Junctions In Fanft‐Induced Carcinomas Of Rat Urinary Bladder In Tissue Culture: In Situ Thin‐Section, Freeze‐Fracture, And Scanning Electron Microscopy Studies

Intercellular Junctions In Fanft‐Induced Carcinomas Of Rat Urinary Bladder In Tissue Culture: In Situ Thin‐Section, Freeze‐Fracture, And Scanning Electron Microscopy Studies

Estimation of viability of bovine granulosa cells

Microcarrier Cell Culture

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