Studies on pulmonary surfactant

Rooney, Seamus A.; Gobran, Laurice I.; Gross, Ian; Wai-Lee, Theresa S.; Nardone, Linda L.; Motoyama, Etsuro K.

doi:10.1016/0005-2760(76)90083-7

Cited by 72 publications

(5 citation statements)

References 30 publications

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“…In the present studies, brush-border membranes of dexamethasone-treated rats were also characterized by alterations in the composition of certain fatty acids in the PC fraction of their membrane phospholipids. Furthermore, in agreement with results of previous studies performed on the effects of corticosteroids on rabbit lung tissue (Rooney et al, 1976), concomitant with these lipid-compositional changes, the specific activities of lysophosphatidylcholine acyltransferase(s), with linoleoyl-CoA and arachidonic-CoA as acyl donors, were found to be increased by dexamethasone administration. It would therefore appear reasonable to suggest that dexamethasone-induced increases in these enzymic activities may, at least in part, be responsible for the fatty acid changes noted in the PC fraction of treated membranes.…”

Section: Discussionsupporting

confidence: 91%

“…In agreement with results ofprevious studies performed on rabbit lung (Rooney et al, 1976), dexamethasone was also found to decrease the sphingomyelin/PC molar ratio of proximal-small-intestinal brush-border membranes by increasing the levels of PC in the treated membranes. Previous studies in rabbit (Rooney et al, 1976) and rat fetal lung (Post et al, 1986) demonstrated that glucocorticoid administration stimulated the activity of cholinephosphate cytidylyltransferase, but had no effect on the activities of pulmonary choline kinase and choline-phosphotransferase. Whether similar alterations in cholinephosphate cytidylyltransferase activity are responsible for the increases in PC seen in the brushborder membranes of treated animals in the present study must await further experiments.…”

Section: Discussionsupporting

confidence: 91%

“…Previous studies in other tissues have suggested that corticosteroids might influence the enzymic activities involved in the fatty acyl deacylation-reacylation cycle of PC (Rooney et al, 1976;Hirata et al, 1980). In view of dexamethasone-induced alterations noted in the fatty acids of this fraction of brush-border membranes (Table 5), we decided to examine and compare these enzymic activities in intestinal homogenates of treated and control Table 5.…”

Section: Effect Of Dexamethasone On Enzymic Activitiesmentioning

confidence: 99%

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Dexamethasone-induced alterations in lipid composition and fluidity of rat proximal-small-intestinal brush-border membranes

Brasitus

Dudeja

Dahiya

et al. 1987

Biochemical Journal

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A series of experiments were conducted to examine the possible effects of subcutaneous administration of the synthetic glucocorticoid dexamethasone (100 micrograms/day per 100 g body wt.) on the lipid fluidity and lipid composition of rat proximal-small-intestinal brush-border membranes. After 4 days of treatment, membranes and their liposomes prepared from treated animals possessed a greater fluidity than did their control (diluent, 0.9% NaCl) counterparts, as assessed by steady-state fluorescence-polarization techniques using several different fluorophores. Examination of the effects of temperature on the anisotropy values of 1,6-diphenylhexa-1,3,5-triene, using Arrhenius plots, moreover, revealed that the mean break-point temperatures of the treated preparations were approx. 3-4 degrees C lower than those of their control-preparation counterparts. Changes in the sphingomyelin/phosphatidylcholine (PC) molar ratio as well as in certain of the fatty acids of the PC fraction of treated membranes, secondary to alterations in membrane PC levels and in lysophosphatidylcholine acyltransferase activities respectively, were also noted after dexamethasone administration. These compositional alterations appeared to be responsible, at least in part, for the differences in fluidity noted between treated and control plasma membranes. These results therefore demonstrate that dexamethasone administration can modulate the lipid fluidity and lipid composition of rat proximal-small-intestinal brush-border membranes.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionsupporting

confidence: 91%

Section: Effect Of Dexamethasone On Enzymic Activitiesmentioning

confidence: 99%

See 1 more Smart Citation

Dexamethasone-induced alterations in lipid composition and fluidity of rat proximal-small-intestinal brush-border membranes

Brasitus

Dudeja

Dahiya

et al. 1987

Biochemical Journal

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“…Studies of normal foetal lung development have shown that PG appears in amniotic fluid during the last weeks of pregnancy and increases in concentration in the fortnight before birth in particular [39]. The concentration of PG in LS can be increased by the administration of cortisol [40]. PG itself can be used as a treatment for RDS partly because it prevents alveolar epithelial apoptosis and profibrotic stimulation [41].…”

Section: Lung Surfactantmentioning

confidence: 99%

Is phosphatidylglycerol essential for terrestrial life?

Furse

2016

J Chem Biol

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Lipids are of increasing importance in understanding biological systems. Lipids carrying an anionic charge are noted in particular for their electrostatic interactions with both proteins and divalent cations. However, the biological, analytical, chemical and biophysical data of such species are rarely considered together, limiting our ability to assess the true role of such lipids in vivo. In this review, evidence from a range of studies about the lipid phosphatidylglycerol is considered. This evidence supports the conclusions that this lipid is ubiquitous in living systems and generally of low abundance but probably fundamental for terrestrial life. Possible reasons for this are discussed and further questions posed.

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“…Antenatal induction of phosphatidylcholine synthesis in the lung of several animal species can be achieved by corticosteroid administration (Farrell & Zachman, 1973), and increased surfactant secretion into alveolar spaces in the foetal lung has been observed after thyrotropin-releasing hormone (Rooney et al, 1979) or cholinergic stimulation (Corbet et al, 1977). The acceleration of pulmonary maturation by corticosteroids, in part, is related to increased activity of cholinephosphotransferase (Farrell & Zachman, 1973), lysophosphatidylcholine acyltransferase (Rooney et al, 1976), and glycerophosphate phosphatidyltransferase (Rooney et al, 1975), and to increased incorporation of glucose into phosphatidylcholine (Gilden et al, 1977).…”

mentioning

confidence: 99%

Phosphatidylcholine synthesis in the developing small intestine

Holtzapple¹,

Starr²,

Morck³

1980

Biochemical Journal

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1. Phosphatidylcholine synthesis in the foetal, newborn and adult small intestine of rats was studied by determination of cytidine diphosphocholine-1,2-diacylglycerocholine phosphotransferase (cholinephosphotransferase) and acyl-CoA-1-acyl-sn-glycerol-3-phosphocholine acyltransferase (lysophosphatidylcholine acyltransferase) activities and the incorporation of [1-14C]oleic acid into phosphatidylcholine. 2. Cholinephosphotransferase activity was low in foetal jejunum and ileum, increased 3-4 fold in the ileum by 6 days of age and by 12 days in the jejunum. Jejunal activity remained constant throughout weaning; ileal activity gradually decreased to values 25% of that of the jejunum. 3. Lysophosphatidylcholine acyltransferase activity was high in foetal jejunum and ileum, decreased 70% immediately after birth in the jejunum and increased to adult values by 12 days of age. Ileal activity decreased by 20% after birth, but decreased more rapidly at weaning to 30% of the activity in jejunum. 4. Initial rates and steady-state incorporation of [1-14C]oleic acid into phosphatidylcholine by jejunal rings of 10 day-old rats exceeded that observed in jejunal rings from adult rats by 2-4-fold. 5. In the postnatal jejunum, neither cholinephosphotransferase and lysophosphatidylcholine acyltransferase activities nor oleic acid incorporation were stimulated by cortisone administration in vivo.

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Studies on pulmonary surfactant

Cited by 72 publications

References 30 publications

Dexamethasone-induced alterations in lipid composition and fluidity of rat proximal-small-intestinal brush-border membranes

Dexamethasone-induced alterations in lipid composition and fluidity of rat proximal-small-intestinal brush-border membranes

Is phosphatidylglycerol essential for terrestrial life?

Phosphatidylcholine synthesis in the developing small intestine

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