Nitrogen is a major pollutant from food processing industries. Handling nitrogen pollutants is a tedious cost-effective job. The newer technologies like anaerobic ammonium oxidation (anammox) shows promising future yet lack in technical clarification of operational details and dependency at different geolocations. The start-up of the anammox process reported at a wide range of operational condition is still ambiguous. This study will attempt to ensure the investigation of uniformly operational physical parameters for the rapid start-up of anammox. This experiment was conducted in a batch culture where three 1L culture bottle was inoculated with 100 ml sludge of 10000mg/L VSS and 900 ml optimized anammox media. The pH of the bottles was adjusted to 6.5, 7.5 and 8.5. The three sets were cultured at 25 o C-55 o C for 3 cycles and removal of ammonia and nitrite was considered as a function of optimal cellular activity of anammox bacteria. Since the growth rate of the Planctomycetes group is very slow this was omitted for the active parameter for optimization. The Box-Behnken full factorial design was considered to derive the optimal point of reaction. The result shows that the removal efficiency for both ammonia and nitrite was exponentially increased from 25-40 0 C, removing 88% of ammoniacal nitrogen and complete removal of nitrite at a pH of 7.5. The ability to remove nitrogen drastically reduced with increasing temperature beyond 40 0 C and pH 7.8. The optimization of parameters suggests optimal operation at 39.7 0 C at a pH of 7.2 for 61.83 h. This result will help in successful startup and operation of anammox reactor.