Studies on selected corallinaceae (Rhodophyta) and other algae in a defined marine culture medium

Woelkerling, William J.; Spencer, Kenneth G.; West, John A.

doi:10.1016/0022-0981(83)90135-1

Cited by 31 publications

(22 citation statements)

References 16 publications

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“…Once in the laboratory U]va blades were preincubated for 2 days in dim light with artificial seawater (Woelkerling et al, 1983) enriched with 26"5 #M of NH + and 2 #M of phosphate. NH + is an abundant source of N in this estuary, reaching transient peaks much higher than NO3-N. Discs (10 mm diameter) of the two Ulva species were cultured in a light-and temperaturecontrolled growth chamber (Koxka, EC 540F) (12:12 h light : clark cycle, 15 °C) for 6 days in aerated I dm 3 flasks with artificial seawater (as above) at different photon fluence rates (PFR): 0, 2, 11, 30, 60 and 200 #molm -z s -I (/~ = 400-700nm) (measured with a LiCor LI-1000 radiometer, with a 2re collector LiCor LI 193 SA).…”

Section: Plant Materials and Experimental Designmentioning

confidence: 99%

The effect of photoacclimation on the photosynthetic physiology ofUlva curvataandUlva rotundata(Ulvales, Chlorophyta)

Pérez‐Lloréns

Vergara

Pino

et al. 1996

European Journal of Phycology

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The effects of photoacclimation on growth, photosynthesis, pigment content and elemental composition of Ulva curvata and Ulva rotunda& which grow together in eutrophic areas of southern Spain, were investigated. CuItures were grown for 6 days at different photon fluence rates (PFR) ranging from darkness up to 200 #mol m -2 s-1 under nutrient-sufficient conditions (artificial seawater supplemented with ammonium and phosphate). Growth rates were not light-saturated (up to 200 #mol m -2 s-l), reaching a value close to 0"2 d -I. Growth rates based on mass, area or C content were equivalent, except in darkness and very low light levels (2 #molm -2 s 1), where thallus expansion occurred by diluting internal biomass and C. Chlorophyll and absorptance showed a bell-shaped PFR-response curve, with maxima at 30-60#molm -2 s 1 and lower values at light saturation and under light-limiting conditions. Although net NH4 + incorporation was not affected by growth-PFR, there was inefficient assimilation of N at low light levels, which may restrict chlorophyll, protein and membrane synthesis. The light-saturated photosynthesis rate (Pm~x) displayed a bell-shaped PFR-response curve, when expressed on an area basis, whereas it was saturated from 1I #molm 2 s-1 when scaled to internal N. This suggests that ribulose-I,5-bisphosphate carboxylase/oxygenase activity could be the rate-limiting step underlying Pm~x for high-light-acclimated algae, whereas electron transport chain elements may limit Pmax under light-limiting conditions. Pma~ and tissue C were always higher for U. curvata. Dark respiration rates were positively correlated with growth rates, and photon yield of net growth declined with increasing growth-PFR. The results are also discussed in relation to cell size, since U. curvata cells are smaller than those of U. rotundata.

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Section: Plant Materials and Experimental Designmentioning

confidence: 99%

The effect of photoacclimation on the photosynthetic physiology ofUlva curvataandUlva rotundata(Ulvales, Chlorophyta)

Pérez‐Lloréns

Vergara

Pino

et al. 1996

European Journal of Phycology

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“…The substratum consisted of an agar-solidified root-rhizome layer (2% agar w/v). Unenriched artificial seawater (marine culture medium without nitrate and phosphate; Woelkerling et al 1983) was used as a solvent. The crystallyzer with the plants was placed in a methacrylate jar (900 ml) filled with filtered (Whatman GF/C) natural seawater.…”

Section: Methodsmentioning

confidence: 99%

Assessing the toxicity of ammonium pulses to the survival and growth of Zostera noltii

Brun

Hernández²,

Vergara³

et al. 2002

Mar. Ecol. Prog. Ser.

124

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The present study assessed the effect of seawater ammonium enrichment on survival and growth of the seagrass Zostera noltii Hornem. Ammonium enrichment had an inhibitory effect on shoot, rhizome and root elongation rates, as well as on primary production. The inhibitory effect was partially alleviated by phosphate addition. The frequency of ammonium pulses also affected growth negatively, with a maximum effect at both low and high pulse frequencies. Similar results were obtained when the experiment was run in the field during a winter trial. However, when the field experiment was repeated in spring at higher mean temperature and irradiance levels, opposite results were obtained, with ammonium enrichment causing a substantial increase in growth rates and productivity. These responses were related to the internal balance of non-structural carbohydrates (especially sucrose) with respect to C and N cell metabolism. Thus, there was a mobilisation of sucrose in both above-and belowground tissues to meet C increased demands arising from ammonium assimilation in winter, with sucrose concentrations reaching critical levels in relation to the total internal C pool. In contrast, sucrose accumulated in the tissues when ammonium pulses were applied in spring, indicating an enhanced C turnover that was able to meet the increased demands arising from ammonium assimilation into organic N compounds.

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“…Experiments were conducted in artificial seawater (ASW; Woelkerling et al 1983) with different initial pulses of NH,' for 6 h, a control treatment without any N supply (0 M, Treatment 1, T1) and with 3 concentrations of NH, ' (50, 100 and 200 M, T2, T3 and T4, respectively). After that period, in a second phase, algae were transferred to N-limited conditions (ASW without N) for 48 h in a 14 h light: l 0 h dark photoperiod cycle.…”

Section: Methodsmentioning

confidence: 99%

Nitrogen assimilation following NH4+ pulses in the red alga Gracilariopsis lemaneiformis:effect on C metabolism

Jj¹,

Kt²,

Fx³

1995

Mar. Ecol. Prog. Ser.

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Nitrogen assimilation following NH4+ pulses in the red alga Gracilariopsis lemaneiformis: effect on C metabolism Juan J. V e r g a r a l , Kimon T. ~i r d~, F. X. ~i e l l~ ABSTRACT: The short-term effect of external NH,' availability on N assimilation, as well as on C metabolism, has been studied in the red alga Gracilariopsis lemaneiformis. This agarophyte seaweed showed a high capacity to take up external NH,' and to channel it toward proteins. As in a previous study in Corallina elongata, it appears that N limitation redirected the flow of internal N toward nonpigmented proteins, whereas phycobiliprotein synthesis was preferentially stimulated with respect to other proteins in response to external NH,+ pulses. A possible mechanism by which chloroplastic protein synthesis is limited by external N availability more than cytosolic proteins could be involved. In relation to C metabolism, insoluble and soluble carbohydrates were mobilized in response to NH,+ assimilation at a non-saturating irradiance for photosynthesls of 80 pm01 m-2 S-'. The main change in C metabolism was not seen for total C. The principal effect was the flow of C among different compounds, from carbohydrates to organic N compounds (amino acids and prote~ns) during transient NH, + assimilation.

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Studies on selected corallinaceae (Rhodophyta) and other algae in a defined marine culture medium

Cited by 31 publications

References 16 publications

The effect of photoacclimation on the photosynthetic physiology ofUlva curvataandUlva rotundata(Ulvales, Chlorophyta)

The effect of photoacclimation on the photosynthetic physiology ofUlva curvataandUlva rotundata(Ulvales, Chlorophyta)

Assessing the toxicity of ammonium pulses to the survival and growth of Zostera noltii

Nitrogen assimilation following NH4+ pulses in the red alga Gracilariopsis lemaneiformis:effect on C metabolism

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