Studies on sodium-potassium-activated adenosinetriphosphatase

Bonting, S.L.; Caravaggio, Leo L.; Canady, Mel Rose; Hawkins, Naomi M.

doi:10.1016/0003-9861(64)90155-9

Cited by 72 publications

(15 citation statements)

References 33 publications

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“…Ouabain, administered by a retrograde injection into the ducts, abolishes both secretion and the potential difference and it was therefore thought that a pump extrudes Na+ across the luminal membrane of the cell. Bonting, Caravaggio, Canady & Hawkins (1964) considered that the intracellular concentrations of ions would probably be similar to those reported for other tissues and agreed that the Na+/K+-activated ATP-ase present in the avian salt-gland must be situated on the luminal membrane. Van Rossum (1966) analysed salt-gland slices and assuming intracellular K to be at a concentration of 150 m-equiv/l.…”

Section: Introductionmentioning

confidence: 91%

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Intracellular concentrations of sodium, potassium and chloride in the salt‐gland of the domestic goose and their relation to the secretory mechanism

Peaker¹

1971

The Journal of Physiology

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SUMMARY1. The composition of the nasal salt-glands of geese was found to be Na 57 + 3-5 (s.E.), K 52-3 + 3-9 and Cl 78-3 + l1@0 m-equiv/kg fresh tissue.During secretion, the Na content was significantly raised to 72-4+3-4 m-equiv/kg. 3. Ouabain (104 M) significantly decreased the tissue and cell K concentration and significantly increased the Na concentration.4. Acetylcholine (10-6 M) and eserine (104 M) in the incubation medium had no effect on intracellular composition.5. Raising the Na concentration of the medium to 172 m-equiv/l. and the Cl to 156 m-equiv/l. in two experiments had -no effect on the calculated intracellular composition.6. These results do not support reports that the cells have a very high Na concentration (about 350 m-equiv/l. intracellular water). They are compatible with the hypothesis that the hypertonic secretion is formed across the luminal membrane of the secretary cell by an active Na+ pump and there are no data to suggest that Na+ is concentrated across the basal membrane by a ouabain-insensitive process.7. The data are discussed in relation to permeability studies and to electrical potential measurements within the gland by other workers.

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Section: Introductionmentioning

confidence: 91%

“…The gland contains a Na±/K+-activated ATP-ase (see, for example, Bonting et al 1964), the amount of which can be related to secretary capacity (Ernst, Goertemiller & Ellis, 1967;Fletcher, Stainer & Holmes, 1967;Ballantyne & Wood, 1970). 6.…”

Section: Peaker Unpublished Observations)mentioning

confidence: 99%

Intracellular concentrations of sodium, potassium and chloride in the salt‐gland of the domestic goose and their relation to the secretory mechanism

Peaker¹

1971

The Journal of Physiology

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“…This paper deals with the characteristics of the (Mg-Ca)-dependent, ouabain-insensitive ATPase first described by Bonting et al (1964) and Frank and Goldsmith (1965).…”

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confidence: 98%

Properties of a magnesium- or calcium-dependent adenosine triphosphatase from frog rod photoreceptor outer segment disks and its inhibition by illumination

Ostwald¹,

Heller²,

Lawrence³

1972

Biochemistry

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Isolated frog retinal rod photoreceptor disk membranes were found to possess an ouabain-insensitive adenosine triphosphatase (ATPase) which was dependent on the presence of Mg or Ca in the incubation medium. Several tests showed the activity was not of mitochondrial origin, although the level of mitochondrial contamination was about 5%. The level of ATPase activity was optimal at an Mg : ATP ratio of 1 :I. Ca supported a lower level of activity in the absence of Mg, showed no optimum Ca:ATP ratio in the range tested, and inhibited ATPase activity when added in the presence of Mg. The released inorganic phosphate was not due to an T he retinal photoreceptor outer segment is the site of the absorption of light in vision. The rod outer segment contains freely floating membranous organelles in the form of a stack of flattened disks. The membranes of these disks contain, as an integral part, the visual pigment rhodopsin which absorbs light and initiates vision. It has been shown previously that the disks contain a distinct internal space, separate from the intracellular space (Heller et a/., 1971) and that the membranes are semipermeable (Heller et a/., 1970).As a part of the biochemical study of these membranous organelles, we performed an electron microscopical and a chemical analysis of the isolated rod outer segment preparation, and then reinvestigated the known adenosine triphosphatase (ATPase)' activity of the preparation. This paper deals with the characteristics of the (Mg-Ca)-dependent, ouabain-insensitive ATPase first described by Bonting et al. (1964) and Frank and Goldsmith (1965).In most respects our results confirm and extend those already described. However, we found a distinct change in ATPase activity upon illumination, in contrast to the results of either Bonting et al. (1964) or Frank and Goldsmith (1965). Methods and MaterialsRod outer segments (ROS) were prepared fresh each day from Rana pipiens which were dark adapted overnight, as described previously (Heller et al., 1971). For experiments in imidazole buffer, the purified outer segments were washed three times, after the sucrose isolation procedure, in 60 mM

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“…At 370 C the CA activity would probably be five times greater (28). CA activity of a similar order of magnitude has been found in the kidney of man (29) and rat (30), and in erythrocytes (17), choroid plexus (31), and the salt gland of the herring gull (32). In the dog liver, by-contrast, any activity present was less than the sensitivity of the method could detect.…”

Section: Discussionmentioning

confidence: 65%

Experimental Metabolic Acidosis: The Enzymatic Basis of Ammonia Production by the Dog Kidney*

Pollak¹,

Mattenheimer²,

DeBruin³

et al. 1965

J. Clin. Invest.

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Ammonia excretion by the kidney has long been known to increase when a constant acid load is given to healthy subjects. This was clearly delineated by Sartorius, Roemmelt, and Pitts (1), who reported that when healthy humans were given a constant amount of ammonium chloride daily, there was a gradual increase in the excretion of ammonia, accompanied by an increased excretion of protons, and by an adaptation that resulted in the conservation of sodium and potassium ions.These experiments were preceded by those of Van Slyke and his co-workers (2), who had demonstrated that glutamine was extracted from the renal arterial blood of acidotic dogs in amounts sufficient to account for about 60% of the NH3 excreted by the kidneys-an observation recently confirmed and extended by the experiments of Pitts and his colleagues (3, 4). The infusion of a variety of amino acids into the acidotic dog has also been shown to increase the rate of excretion of ammonia (54), L-glutamine, L-asparagine, Land D-alanine, and L-histidine being the most effective.The nature of the metabolic pathways involved and the enzymes responsible for catalyzing the relevant reactions are not yet clearly understood.

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Studies on sodium-potassium-activated adenosinetriphosphatase

Cited by 72 publications

References 33 publications

Intracellular concentrations of sodium, potassium and chloride in the salt‐gland of the domestic goose and their relation to the secretory mechanism

Intracellular concentrations of sodium, potassium and chloride in the salt‐gland of the domestic goose and their relation to the secretory mechanism

Properties of a magnesium- or calcium-dependent adenosine triphosphatase from frog rod photoreceptor outer segment disks and its inhibition by illumination

Experimental Metabolic Acidosis: The Enzymatic Basis of Ammonia Production by the Dog Kidney*

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