Studies on Some Chemical Components of Nutmeg (Myristica Fragrans-Houtt) Leaf Directed at Determination of Sex of Seedlings

Packiyasothy, E.V.; Jansz, E.R.; Dharmadasa, H M

doi:10.4038/jnsfsr.v19i2.8156

Cited by 2 publications

(1 citation statement)

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“…Phadnis and Choudhary [23] advocated a colorimetric test that they claimed could distinguish sex in approximately two thirds of seedlings while Nayar et al [24] examining the epidermal cells of nutmeg leaf described the different structures of calcium oxalate crystals in seedlings of male and female plants. In a preliminary study, it was indicated that characteristics such as leaf shape, essential oil content and composition and profile of phenolics differ in male and female plants [25]. Contrarily, a study conducted by Mezencev et al [26] confirmed the absence of obvious relationship between the extents of morphological variations and that of changes at the DNA level already reported in rice.…”

Section: Conventional Sex Diagnosticsmentioning

confidence: 83%

Sex Determination in Nutmeg Seedlings Using Scar Primers

Dadzie

2018

JHPR

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Myristica fragrans H. is a dieocious plant with male and female flowers on different trees. At the juvenile stage of nutmeg, their sexes is not morphologically identified until at the flowering stage. However, the female is more economical than the male plants. This challenge is making the work of nutmeg farmers difficult and also inhibiting the development of the spice and flavouring industry in Ghana. Hence, the use of molecular technique to characterize male and female nutmeg plants. SCAR markers are easy, specific, and reliable and have a high sense of reproducibility than other markers. Two different primers (F-Napf-76, R-Napf-77 and F-Napf-70, R-Napf-71) were used for the PCR amplification. It was revealed that the total genomic DNA of samples from both mature plant and seedlings of nutmeg were of good quality and was much concentrated by giving good DNA bands. Although there were no distinctions among the bands, they all lie at the same level (0.3kbp) indicating that the samples were of similar molecular weight. However, double PCR amplification was not seen in all samples when run on a 1% agarose gel but single amplifications were observed.

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Section: Conventional Sex Diagnosticsmentioning

confidence: 83%