Studies on structure-function relationships of acetolactate decarboxylase from Enterobacter cloacae

Abstract

Arg150 in E.c.-ALDC exhibited a unique tilted conformation implying the lower activities of E.c.-ALDC comparing to other bacterial ALDCs. Sequence conservation of E.c.-ALDC is represented by ribbons and lines (conserved, magenta to variable, cyan).

Beneficial features of plant growth-promoting rhizobacteria for improving plant growth and health in challenging conditions: A methodical review

“…Based on that knowledge, new biotechnological products may be developed and innovative solutions may be introduced that exploit plant-beneficial bacteria for biological control of plant diseases (biopesticides) and for plant growth promotion (biofertilizers) for sustainable agricultural practices and phytoremediation (Mesa-Marín et al, 2020). Rajkumar et al, 2009;, Glick, 20140;, Rajkumar et al 2010, Ma et al, 2011, Rajkumar et al, 2012;, Salomon et al, 2014;, Zhao and Zhang, 2015;, Hashem et al, 2016;, Zaheer et al, 2016;, Ahmad et al, 2016;, Egamberdieva et al, 2017;, Singh and Jha, 2019;, Kudoyarova et al, 2019 and Glick, 1996;Spaepen and Vanderleyden, 2011;Lin et al, 2015;Goswami et al, 2016 54 Kandasamy et al, 2016;Ji et al, 2018…”

Escherichia coli aceE variants coding pyruvate dehydrogenase improve the generation of pyruvate‐derived acetoin

“…Of these two enzymes, only kinetic parameters for ALDC from E. cloacae ssp. dissolvens are available: the K M is 12.2 mM, and k cat is 0.96 s −1 [ 47 ] while known ALS enzymes have a turnover number 100 times greater [ 48 ]. Even without kinetic parameters for the specific ALS used, the low affinity turnover rate of ALDC, taken together with the observed accumulation of acetolactate, support the hypothesis that ALDC is the rate limiting step for acetoin formation.…”