Studies on swine and canine serum haptoglobins

Shim, Bong-Sop; Yoon, Chang-Soo; Oh, Song Hee; Lee, Tong-Ho; Kang, Young‐Hoon

doi:10.1016/0005-2795(71)90046-8

Cited by 15 publications

(14 citation statements)

References 12 publications

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“…The canine protein showed a molecular mass of 52 kDa in the absence of mercaptoethanol, and under reducing conditions two bands of 38 and 17 kDa appeared, agreeing with previous reports [22,35]. This purification strategy involved fewer chromatographic separations than the methods detailed previously [5,30], reducing the time taken to purify Hp to an acceptable level so this procedure could be widely used for the purification of canine Hp. Stability of the purified protein is one problem to be solved, since we have noticed that after a few weeks of storage the protein loses its reactivity.…”

Section: Discussionsupporting

confidence: 89%

Development of a time-resolved fluorometry based immunoassay for the determination of canine haptoglobin in various body fluids

Parra

Väisänen

Cerón³

2005

Vet. Res.

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-A time-resolved immunofluorometric assay (TR-IFMA) was developed for the determination of haptoglobin (Hp) in canine serum. Haptoglobin was purified from canine acute phase serum by ammonium sulphate precipitation followed by gel filtration. This isolated dog Hp was used as the standard to calibrate the assay. Intra-and inter-assay coefficients of variation of the assay were, respectively, 5.7% and 16.6% at 0.51 mg/mL, 2.4% and 10.6% at 2.1 mg/mL and 10.5% and 11.9% at 32.5 mg/mL. The dilution of serum samples with high Hp concentrations resulted in linear regression equations with R 2 of 0.99 and 0.97. A high correlation was found in serum Hp measurements by TR-IFMA and a commercial assay based on peroxidase activity of haemoglobin bound to haptoglobin (R 2 = 0.96). The limit of detection for the TR-IFMA method was 0.002 µg/mL. The addition of fresh haemolysate to serum samples did not affect the haptoglobin concentration (P = 0.694). Statistical differences (P < 0.003) were found between healthy dogs and dogs with different pathological processes. In whole blood, Hp concentrations were much lower than in serum but closely related (R 2 = 0.84) whereas saliva Hp concentrations were poorly related with serum concentrations (R 2 = 0.53). However, the concentration of Hp in saliva was significantly (P < 0.039) higher in dogs with pathological processes compared to healthy dogs. The assay sensitivity was adequate to also be applied to whole blood and saliva specimens.canine / haptoglobin / time-resolved fluorometry / whole blood / saliva

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Section: Discussionsupporting

confidence: 89%

Development of a time-resolved fluorometry based immunoassay for the determination of canine haptoglobin in various body fluids

Parra

Väisänen

Cerón³

2005

Vet. Res.

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“…Porcine Hp has an electrophoretic mobility similar to that of human Hp phenotype 1-1 [106]. The molecular weight of porcine Hp has been estimated to be approximately 120 kDa [64,106,161,178]. Bovine Hp was found to consist of monomers of 16 to 23 kDa (α-chains) and 35 to 40 kDa (β-chains) [37,118] and to exist as a polymer in association with albumin with a molecular weight above 1 000 kDa in cattle serum [37].…”

Section: Haptoglobinmentioning

confidence: 99%

Application of acute phase protein measurements in veterinary clinical chemistry

2004

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-The body's early defence in response to trauma, inflammation or infection, the acute phase response, is a complex set of systemic reactions seen shortly after exposure to a triggering event. One of the many components is an acute phase protein response in which increased hepatic synthesis leads to increased serum concentration of positive acute phase proteins. The serum concentration of these acute phase proteins returns to base levels when the triggering factor is no longer present. This paper provides a review of the acute phase proteins haptoglobin, C-reactive protein and serum amyloid A and their possible use as non-specific indicators of health in large animal veterinary medicine such as in the health status surveillance of pigs at the herd level, for the detection of mastitis in dairy cattle and for the prognosis of respiratory diseases in horses.

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“…A haptoglobina (Hp) é uma globulina glicoproteica de peso molecular de 81 kDa (SHIM et al, 1971; PETER-SEN; NIELSEN; HEEGAARD, 2004; CÉRON; ECKER-SALL; MARTINEZ-SUBIELA, 2005;SHEAHAN et al, 2010).…”

Section: Haptoglobinaunclassified

Proteínas De Fase Aguda Em Cães E Gatos

Santos

Alberto²

2015

ArqVet

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RESUMO:As proteínas de fase aguda (PFA) são um grupo de proteínas sanguíneas que apresentam alterações nas suas concentrações em animais acometidos por infecções, inflamações, trauma cirúrgico ou mesmo submetido ao estresse. São proteínas que alteram as suas concentrações em pelo menos 25% durante a inflamação. As PFA consistem em proteínas de fase aguda negativa e/ou positiva, diminuindo ou aumentando a sua concentração, respectivamente, em resposta a um estí-mulo inflamatório. Dentre as PFA negativas mais importantes estão a albumina e a transferina. As PFA positivas são a haptoglobina (Hp), proteína C-reativa (CRP), amiloide A-sérico (SAA), ceruloplasmina (Cp), fibrinogênio e a alfa 1 glicoproteina ácida(APG). As avaliações da concentração das PFA e proteínas totais propiciam subsídios para adequada interpretação do estado de hidratação, bem como de inflamação, infecção, doença imunomediada e alteração da síntese proteica. Sendo as PFA mediadores inflamatórios das respostas imunes agudas, e consideradas marcadoras das lesões teciduais na sua fase aguda nos animais, é importante realizar uma revisão sobre as PFA mais importantes e suas funções nos cães e gatos. PALAVRAS-CHAVE: Dor. Estresse. Inflamação. Pequenos animais. Resposta inflamatória aguda. ACUTE-PHASE PROTEINS IN DOGS AND CATSABSTRACT: Acute-phase proteins (APP) are a group of blood proteins exhibiting changes in their concentrations in animals suffering from infections, inflammation, surgical trauma or even which have been subjected to stress. These proteins present at least 25% changes in their concentrations during inflammation. APPs consist of negative-and positive-phase proteins that can decrease or increase their concentration in response to an inflammatory stimulus. The most important negative APP are albumin and transferrin; and the most important positive APP are haptoglobin (Hp) , C-reactive protein (CRP), serum amyloid A (SAA), ceruloplasmin (Cp), fibrinogen and alpha 1 glycoprotein acid (AGP). APP concentration and total protein reviews provide information for proper interpretation of hydration status, as well as inflammation, infection, immune-mediated diseases and impaired protein synthesis. APPs are inflammatory mediators of acute immune responses and are considered markers for tissue damages in the acute phase in animals. Therefore, it is important to further review the most important APPs and their functions in dogs and cats. KEYWORDS: Pain. Stress. Inflammation. Small animals. Acute inflammation response. PROTEÍNAS DE FASE AGUDA EN PERROS Y GATOSRESUMEN: Las proteínas de fase aguda (PFA) son un grupo de proteínas sanguíneas que presentan cambios en sus concentraciones en animales acometidos por infecciones, inflamaciones, trauma quirúrgico o mismo sometido a estrés. Son proteí-nas que alteran sus concentraciones de al menos 25 % durante la inflamación. Las PFA consisten en proteínas de fase aguda negativa y/o positiva, disminuyendo o aumentando su concentración, respectivamente, en respuesta a un estímulo inflamatorio. Entre las PFA...

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Studies on swine and canine serum haptoglobins

Cited by 15 publications

References 12 publications

Development of a time-resolved fluorometry based immunoassay for the determination of canine haptoglobin in various body fluids

Development of a time-resolved fluorometry based immunoassay for the determination of canine haptoglobin in various body fluids

Application of acute phase protein measurements in veterinary clinical chemistry

Proteínas De Fase Aguda Em Cães E Gatos

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