Studies on the absorption of proteins: the amino-acid pattern in the portal blood

Dent, C. E.; Schilling, John A.

doi:10.1042/bj0440318

Cited by 129 publications

(42 citation statements)

References 11 publications

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“…Ug is at least partly dialysable through collodion sacs prepared according to the directions of Dent & Schilling (1949). The sacs were kept as thin as possible in order to minimize loss by adsorption on the wall of the sac (which becomes stained with pigment) and were tested carefully for leaks before and after dialysis.…”

Section: Resultsmentioning

confidence: 99%

A new method for the preparation of urogastrone

Gregory¹

1955

The Journal of Physiology

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Section: Resultsmentioning

confidence: 99%

A new method for the preparation of urogastrone

Gregory¹

1955

The Journal of Physiology

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“…These enzymes have been reported to occur both in brush border membranes and in the soluble cytoplasmic fraction (1)(2)(3)(4)(5)(6). Although data regarding the quantitative distribution of peptide hydrolases in these fractions are lacking, it has been reported that the brush border membrane contains less dipeptide hydrolase activity than the soluble cytosol fraction, while tripeptide hydrolase activity is much more active in the former (4,5).…”

Section: Introductionmentioning

confidence: 99%

“…It is generally accepted that, in the adult, dietary protein appears in the portal circulation primarily as amino acids (6,7). However it is not yet clear whether dietary protein is absorbed from intestinal lumen to mucosa primarily as amino acids or as small peptides.…”

Section: Introductionmentioning

confidence: 99%

Peptide hydrolases in the brush border and soluble fractions of small intestinal mucosa of rat and man

Kim¹,

Birtwhistle²,

Kim³

1972

J. Clin. Invest.

160

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A BST RA CT Peptide hydrolases, catalyzing the hydrolysis of 13 dipeptides and 5 tripeptides into their respective amino acids, were studied in small intestinal mucosa and other tissues, in man and in the rat.Studies on the subcellular distribution of these enzymes showed enzyme activities in both the soluble and brush border fractions of the rat small intestinal mucosa, the former constituting 80-90% and the latter 10-15% of the total activity. Zymogram studies of peptide hydrolases, in both fractions, yielded multiple bands indicating multiple zones of enzyme activity. With most substrates a rather broad range of enzyme activities was observed in the soluble fraction differing only slightly from substrate to substrate, the exception being when L-leucyl-L-proline was used: this latter led to a zymogram pattern which was quite distinct. The synthetic substrates, L-leucyl-P-naphthylamide and Lleucinamide appeared to be hydrolyzed by two electrophoretically distinct enzymes, different from those hydrolyzing other leucyl-containing peptide substrates.Zymogram patterns of the brush border membrane fraction were quite different from those of the soluble fraction of rat small intestine indicating that enzymes from the two sources may be different. No comparable human data were obtained.Peptide hydrolases in the soluble fractions of various organs from the same species gave similar zymogram patterns, while those from the plasma membrane-bound fractions of different organs in the same species were peculiar to each organ. From these data, it is suggested that peptide hydrolases in the brush border and the soluble fractions of small intestine are distinct enzymes and may play different roles in cellular function.

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“…They also noted that changes in concentration of several amino acids in portal blood were not reflected to any great extent in peripheral blood. On the other hand, Dent and Shilling (1949) Unfortunately the data collected in the blood amino acid studies were limited. Animal variation was not measured in these studies.…”

Section: Discussionmentioning

confidence: 99%

“…Later, Dent and Shilling (1949) determined the levels of free plasma amino acids in portal and jugular blood of dogs at 1, 2.5 and 5 hours after feeding protein meals. These workers reported that the concentration of plasma amino acids in sys temic blood was lower than in portal blood; however, changes in systemic blood were fairly proportional to changes noted in portal blood.…”

Section: Portal Versus Systemic Blood Amino Acidsmentioning

confidence: 99%

Influence of protein sources on performance and plasma amino acid patterns of ruminants

Theurer

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Studies on the absorption of proteins: the amino-acid pattern in the portal blood

Cited by 129 publications

References 11 publications

A new method for the preparation of urogastrone

A new method for the preparation of urogastrone

Peptide hydrolases in the brush border and soluble fractions of small intestinal mucosa of rat and man

Influence of protein sources on performance and plasma amino acid patterns of ruminants

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