“…It is now accepted that On the kidney causing inhibition Of water amino-acids in a mixture, by means of two-dimensional f-BUTANOL-ACETIC ACID- Arneil and Wilson (1953) and Valeri, Zacco and Perrini (1953) had suggested that the antidiuretic hormone could be isolated by twodimensional paper chromatography and this was the method employed. In man, however, only a very small fraction of the total hormone stored can be recovered from the hypothalamus and some 99 per cent of the total storage is in the posterior pituitary gland.…”