Studies on the Amino Acids in Yeast RNA in Bound Form: V. The Amino Acids Bound to Guanosine*

Akashi, Shuzo; Murachi, Takashi; Ishihara, Hideko; Goto, Hideo

doi:10.1093/oxfordjournals.jbchem.a128178

Cited by 6 publications

(3 citation statements)

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“…First, there is no change in the absorbance of E. coli tRNA at 330 nm after acylation; and secondly, iodine oxidation of rat liver tRNA does not affect the acylation procedure (Table IVj. Akashi et al (1965) have described the isolation from yeast-soluble R N A of modified guanosine residues containing amino acids in ester linkage to the 2'-hydroxyl group of the ribose moiety. The acylated nucleoside isolated in the present study difl'ers from the expected acylated product of such a modified guanosine residue by its behavior on Dowex 50, its electrophoretic behavior in 1 M formic acid and sodium tetraborate, as well as by its spectrum.…”

Section: Wavtlnalh (Nm)mentioning

confidence: 99%

Acylation of transfer ribonucleic acid with the N-hydroxysuccinimide ester of phenoxyacetic acid

Friedman¹

1972

Biochemistry

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Uncharged rat liver tRNA can be eluted from BDcellulose columns with 0.8 M sodium chloride, with the exception of tRNAPhe, which can only be eluted with 0.8 M sodium chloride containing ethanol. When the uncharged rat liver tRNA is reacted with the N-hydroxysuccinimide ester of phenoxyacetic acid, there is an increase in the amount of tRNA that is eluted in the presence of ethanol. Greater than 5 0 z of the tRNAs for tyrosine, threonine, isoleucine, cysteine, and asparagine can now be eluted only in the presence of ethanol. Only a small fraction of the tRNA from Escherichin coli and yeast shifted into the ethanol fraction after reac-T here have been several reports of the presence of certain bases in transfer ribonucleic acid that can undergo acylation reactions. However, the nature of these bases has not been fully characterized, nor has it been determined whether these are rare bases, or one or more of the four major bases, that are especially reactive because of their conformation in tRNA. With the advent of BD-cellulose' columns, which have affinity

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Section: Wavtlnalh (Nm)mentioning

confidence: 99%

Acylation of transfer ribonucleic acid with the N-hydroxysuccinimide ester of phenoxyacetic acid

Friedman¹

1972

Biochemistry

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“…Examination of the hydrolysate by means of ion-exchange chromatography on a sulfonic acid resin revealed the presence of a A number of articles reports the presence of amino acids or small polypeptides, apart from the amino acids attached to the acceptor end of tRNA molecules, bound to nucleic acids. Ingram and Sullivan (1962) and Akashi et al (1965), for example, have reported the presence of amino acids bound to RNA which cannot be removed through the use of extensive deproteinizing procedures. Balk et al (1964) and Olenick and Hahn (1964) have reported the presence of amino acids in highly purified preparations of DNA isolated from a variety of sources.…”

mentioning

confidence: 99%

Aminoacyl nucleosides. VI. Isolation and preliminary characterization of threonyladenine derivatives from transfer ribonucleic acid

Gb¹,

Rh²,

Mozejko³

et al. 1969

Biochemistry

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Transfer ribonucleic acid was hydrolyzed by acid under conditions that released the purine residues. Examination of the hydrolysate by means of ion-exchange chromatography on a sulfonic acid resin revealed the presence of a A number of articles reports the presence of amino acids or small polypeptides, apart from the amino acids attached to the acceptor end of tRNA molecules, bound to nucleic acids. Ingram and Sullivan (1962) and Akashi et al. (1965), for example, have reported the presence of amino acids bound to RNA which cannot be removed through the use of extensive deproteinizing procedures. Balk et al. (1964) and Olenick and Hahn (1964) have reported the presence of amino acids in highly purified preparations of DNA isolated from a variety of sources. The nature of the amino acid-nucleic acid linkage, however, has not been elucidated. Bogdanov et al. (1962) have reported that tRNA contains amino acids attached to the phosphate residues. Harris and Wiseman (1962) have also reported the presence of small polypeptides attached to the

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“…Mp: 142 °C. 13C NMR (CDC13, ppm): 85.8, (V C), 41.3 (2' C), 72.3 (3' C), 86.6 (4' C), 63.8 (5' C), 163.4 (4 CO), 150.3 (2 CO), 107.3 (5 C), 145.0 (6 C), 34.9 (7 C), 38.6 (8 C), 54.3 (9 C), 31.3 (10 C), 139.2 (11 C), 114.8 (12 C), 136.9 (13 C), 171.6 (14 C), 87.0 (15 C), 27.9 (16 C), 79.8 (17 C), 28.4 NMR (CDClg): 6.35 (t, 1 H, HI', J = 7.0 Hz), 2.4 (m, 2 , H2'), 4.6 (m, 1 , H3'), 4.15 (m, 1 , H4'), 3.45 (m, 2 , H5'), 3.0 (m, 4 , H7 and H8), 4.45 (m, 1 , H9), 3.3 (m, 2 , H10), 1.6 (s, 9 , H16), 1.4 (s, 9 , H18), 3.8 (s, 6 , H20). FABMS m/z 988+, (M + Li).…”

Section: -mentioning

confidence: 99%

Synthesis and characterization of nucleoside peptides: toward chemical ribonucleases. 1

Bashkin¹,

Gard²,

Modak³

1990

J. Org. Chem.

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Studies on the Amino Acids in Yeast RNA in Bound Form: V. The Amino Acids Bound to Guanosine*

Cited by 6 publications

References 0 publications

Acylation of transfer ribonucleic acid with the N-hydroxysuccinimide ester of phenoxyacetic acid

Acylation of transfer ribonucleic acid with the N-hydroxysuccinimide ester of phenoxyacetic acid

Aminoacyl nucleosides. VI. Isolation and preliminary characterization of threonyladenine derivatives from transfer ribonucleic acid

Synthesis and characterization of nucleoside peptides: toward chemical ribonucleases. 1

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