Studies on the anti-proliferative and anti-quorum sensing potentials of Myrtus communis L. essential oil for the improved microbial stability of salmon-based products

Myszka, Kamila; Sobieszczańska, Natalia; Olejnik, Anna; Majcher, Małgorzata; Szwengiel, Artur; Wolko, Łukasz; Juzwa, Wojciech

doi:10.1016/j.lwt.2020.109380

Cited by 13 publications

(11 citation statements)

References 42 publications

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“…Our result was agreed by recent report of Su et al (2020) , and the chemical groups of the shifted places were exhibited the loading efficiency of essential oils into chitosan. Supported evidence was published by K. Myszka, et al (2020) , and the shifted peaks without change of wave number containing chitosan loaded essential oil combination were successful. Therefore, our result was confirmed that the guva essential oils were encapsulated into the chitosan nanoparticles without inclusion of any chemical reaction.…”

Section: Resultssupporting

confidence: 64%

Anti-bacterial activity of chitosan loaded plant essential oil against multi drug resistant K. pneumoniae

Zhang

Ramachandran

Mothana

et al. 2020

Saudi Journal of Biological Sciences

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The development of antibiotic resistant in K. pneumoniae is an emerging thread worldwide due to the poor antimicrobial drugs. To overcome this issue, researchers are focused on plant material and their essential oils to fight against multi drug resistant bacteria. In this context, the current study was concentrated in medicinal plant of guva leaves and their essential oils to combat multi drug resistant bacterial infections. The essential oils were successfully screened and confirmed by HRLC-MS analysis. The anti-bacterial ability of the compounds were loaded into the chitosan nanoparticles and proved by FT-IR analysis. In addition, the chitosan loaded essential oils morphology was compared with chitosan alone in SEM analysis and suggested that the material was loaded successfully. Further, the anti-bacterial ability of the chitosan loaded essential oils were primarily confirmed by agar well diffusion method. At the 100 µg/mL of lowest concentration of chitosan loaded essential oils, the multi-drug resistant K. pneumoniae was inhibited with 96% and confirmed by minimum inhibition concentration experiment. Hence, all the experiments were proved that the essential oils were successfully loaded into the chitosan nanoparticles, and it has more anti-bacterial activity against multi-drug resistant K. pneumoniae.

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Section: Resultssupporting

confidence: 64%

Anti-bacterial activity of chitosan loaded plant essential oil against multi drug resistant K. pneumoniae

Zhang

Ramachandran

Mothana

et al. 2020

Saudi Journal of Biological Sciences

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“…Juniper EO was extracted from dried berries of juniper ( Juniperus phoenicea L.) from Italy by the hydrodistillation method in a Clevenger-type apparatus. Chemical characterization of juniper EO was further carried out by a Hewlett-Packard HP 7890A gas chromatograph coupled to a 5975C mass spectrometer (Agilent, Santa Clara, CA, USA) according to our previous study (Myszka et al 2020 ). Mass spectra were obtained at 70 eV in the mass scanning range of 33–350 m/z.…”

Section: Methodsmentioning

confidence: 99%

“…AHL molecules were also identified by a Dionex UltiMate 3000 (Thermo Fisher Scientific, Waltham, MA, USA) RP-UHPLC chromatograph coupled with ESI-MS with a qTOF system using a Kinetex TM 1.7 Mm C18 100 Å LC column with dimensions of 100 × 2.1 mm (Phenomenex, Torrance, USA) according to our previous study (Myszka et al 2020 ). Mass spectra were recorded in a scan range of m/z 80–1200.…”

Section: Methodsmentioning

confidence: 99%

“…Recent studies have shown that cold-tolerant Pseudomonas spp., mostly Pseudomonas fluorescens , can cause premature deterioration of seafood; these strains are dominant in sardine-, mackerel-, and salmon-based products under cold chain logistics (Venugopal 1990 ; Myszka et al 2020 ). Spoilage activities of P. fluorescens within fish ecosystems may lead to the denaturation of muscle proteins, degradation of lipids, and structural damage to the fish membranes (Ackman 1994 ; Xie et al 2018 ).…”

Section: Introductionmentioning

confidence: 99%

“…Our previous work revealed that pseudomonads under refrigeration conditions can synthesize autoinducers of quorum-sensing (QS) systems (Myszka et al 2020 ; Sobieszczańska et al 2020 ). Autoinducers can interact with cognate receptor proteins, which leads to the expression of specific genes that regulate the spoilage activities of cells (Papenfort and Bassler 2016 ).…”

Section: Introductionmentioning

confidence: 99%

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In situ approaches show the limitation of the spoilage potential of Juniperus phoenicea L. essential oil against cold-tolerant Pseudomonas fluorescens KM24

Myszka

Tomaś

Wolko

et al. 2021

Appl Microbiol Biotechnol

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The present study aimed to elucidate the effect of subinhibitory concentrations (sub-MICs) of juniper essential oil (EO), α-pinene, and sabinene on the quorum-sensing (QS)–mediated proteolytic and lipolytic properties of Pseudomonas fluorescens KM24. These activities were verified under in situ conditions, in which sub-MICs of the agents altered the morphology of KM24 cells. RNA-Seq studies revealed key coding sequences (CDSs)/genes related to QS and the proteolytic/lipolytic activities of pseudomonads. In this work, all the examined agents decreased autoinducer synthesis and influenced the mRNA expression of the encoding acyltransferase genes lptA, lptD, and plsB. The highest reduction on the 3rd and 5th days of cultivation was observed for the genes lptD (−5.5 and −5.61, respectively) and lptA (−3.5 and −4.0, respectively) following treatment with EO. Inhibition of the lptA, lptD, and plsB genes by singular constituents of EO was on average, from −0.4 to −0.7. At 5 days of cultivation the profile of AHLs of the reference P. fluorescens KM24 strain consisted of 3-oxo-C14-HSL, 3-oxo-C6-HSL, C4-HSL, and N-[(RS)-3-hydroxybutyryl]-HSL, the concentrations of which were 0.570, 0.018, 3.744, and 0.554 μg ml−1, respectively. Independent of the incubation time, EO, α-pinene, and sabinene also suppressed the protease genes prlC (−1.5, −0.5, and −0.5, respectively) and ctpB (−1.5, −0.7, and −0.4, respectively). Lipolysis and transcription of the lipA/lipB genes were downregulated by the agents on average from −0.3 to −0.6. α-Pinene- and sabinene-rich juniper EO acts as an anti-quorum-sensing agent and can repress the spoilage phenotype of pseudomonads. Key points: Juniper EO, α-pinene, sabinene exhibited anti-QS potential toward KM24. RNA-Seq revealed key CDSs/genes related to QS/proteolytic/lipolytic activities of KM24. Agents at sub-MIC levels influenced the mRNA expression of QS/lipase/protease genes.

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Effect of black pepper essential oil on quorum sensing and efflux pump systems in the fish-borne spoiler Pseudomonas psychrophila KM02 identified by RNA-seq, RT-qPCR and molecular docking analyses

et al. 2021

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Studies on the anti-proliferative and anti-quorum sensing potentials of Myrtus communis L. essential oil for the improved microbial stability of salmon-based products

Cited by 13 publications

References 42 publications

Anti-bacterial activity of chitosan loaded plant essential oil against multi drug resistant K. pneumoniae

Anti-bacterial activity of chitosan loaded plant essential oil against multi drug resistant K. pneumoniae

In situ approaches show the limitation of the spoilage potential of Juniperus phoenicea L. essential oil against cold-tolerant Pseudomonas fluorescens KM24

Effect of black pepper essential oil on quorum sensing and efflux pump systems in the fish-borne spoiler Pseudomonas psychrophila KM02 identified by RNA-seq, RT-qPCR and molecular docking analyses

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