Studies on the antitumor potentials of betulinic acid against murine ascites Dalton's lymphoma

Bhaumik, Anamika; Prasad, Surendra

doi:10.18203/2319-2003.ijbcp20162538

Cited by 3 publications

(1 citation statement)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Ascites DL tumor was maintained in vivo in 10-12 w old mice of both sexes weighing 25-30 g by serial intraperitoneal (ip) transplantation of viable tumor cells (1×10 7 cells in 0.25 ml phosphatebuffered saline (PBS, pH 7.4) to the animals as per the established procedure in the lab. Following tumor transplantation, an increase in abdomen size and body weight associated with sluggish movement of animals was noted from 3-4 d onwards, which was an early sign of tumor progression and malignancy [19,20] . Tumor-transplanted hosts usually survive for 19-21 d. The maintenance, use of the mice and the experimental protocols has been approved by the Institutional Animal Ethics Committee (No.…”

Section: Tumor Maintenance and Drugs Treatment Schedulementioning

confidence: 99%

Rutin-mediated Apoptosis and Glutathione Changes in Ascites Daltons Lymphoma Cells: In silico Analysis of Rutin Interactions with Some Antiapoptotic and Glutathione-related Proteins

Prasad¹,

Banerjee²,

Kharshiing³

et al. 2019

pharmaceutical-sciences

View full text Add to dashboard Cite

Rutin or rutoside or sophorin is quercetin-3-rutinoside, a citrus flavonoid glycoside found in a wide variety of plants and has been reported to exhibit anticancer effect against many cancers [1-3]. Molecular docking is a frequently used tool in computer-aided structure-based rational drug design, which may help in evaluating how a small molecule called ligand and the target macromolecule (proteins/enzymes) fit together [4,5]. The anticancer activity of rutin involves inhibition of cell proliferation, a decrease in reduced glutathione (GSH) and induction of apoptosis in cancer cells [2,6]. Cis-diamminedichloroplatinum (II), (CDDP) is a well-known platinum-based cancer chemotherapeutic drug and its anticancer properties have been credited to its DNA binding ability, induction of apoptosis and decrease in glutathione level in cancer cells [7-9]. Therefore, to have a comparative study, CDDP was used as a reference anticancer drug in the present study. Apoptosis is a genetically regulated programmed cell death and the efficacy of anticancer drugs is often measured by their ability to selectively promote apoptosis in cancer cells [10]. B-cell lymphoma-extralarge (Bcl-xL), a member of the Bcl-2 family of proteins, is an antiapoptotic protein, which leads to caspase activation and ultimately, programmed cell death [11]. c-FLIP (cellular FLICE, a FADD-like IL-1β-converting enzyme-inhibitory protein) is a major antiapoptotic protein and an important factor that suppresses cytokine-and chemotherapy-induced apoptosis [12]. GSH, a tri-peptide of L-cysteine, L-glutamic acid and glycine, plays important roles in antioxidant defense, nutrient metabolism, and regulation of cellular processes, including cell differentiation, proliferation and apoptosis [13]. Glutathione S-transferases (GST) catalyzes the conjugation of GSH to xenobiotic substrates for the purpose of detoxification. Glutathione reductase (GR) catalyzes the reduction of glutathione disulphide to GSH [14] .

show abstract

Section: Tumor Maintenance and Drugs Treatment Schedulementioning

confidence: 99%