Studies on the Antiviral Activity of Distamycin A

Gh, Werner; Ganter, P; Ratuld, Y. De

doi:10.1159/000220346

Cited by 28 publications

(7 citation statements)

References 6 publications

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“…Even the inhibition of DNA uptake, genetic transformation, and phage DNA transfection in Bacillus subtilis have been shown to be due to an interaction between distamycin and competent cells rather than with donor DNA (15). This interpretation fits with other experimental work (8,19) that indicated the cell surface as the probable site of action of the antibiotic.…”

supporting

confidence: 85%

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Genetic and Physiological Studies on the Site of Action of Distamycin A

Siccardi

Lanza

Nielsen

et al. 1975

Antimicrob Agents Chemother

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Two new genetic loci of Bacillus subtilis are identified by mutations that confer resistance to distamycin A and to other antibacterial agents. The chromosomal region where they map probably contains a cluster of genes whose products are related to membrane structure and function. Some of the biological effects of distamycin A are still in evidence in the resistant mutants indicating that the drug possibly acts at multiple sites. Most biological effects of the drug (including the phenotypic correction of a morphopoietic mutation) are likely to be due to the interaction of distamycin A with membrane (or surface) structures.

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supporting

confidence: 85%

“…6C). DISCUSSION The basic oligopeptide distamycin A interacts (14,15,21) with DNA in vitro, but most of the biological effects it displays seem to be unrelated to this property and due to other interactions between the antibiotic and surface structures (15,19).…”

Section: Resultsmentioning

confidence: 99%

Genetic and Physiological Studies on the Site of Action of Distamycin A

Siccardi

Lanza

Nielsen

et al. 1975

Antimicrob Agents Chemother

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“…The final effect is a reduction of the affinity of receptor sites for DNA. The possibility of an interaction between distamycin and some element of the envelope was also indicated by previous reports (4,5,13,14). It will not be surprising if additional studies indicate that the "in vivo" action of the drug is quite unrelated to its affinity for DNA.…”

Section: Methodsmentioning

confidence: 54%

Interaction Between Deoxyribonucleic Acid and Distamycin A Studied by Transformation in Bacillus subtilis

Mazza

Galizzi

Minghetti

et al. 1973

Antimicrob Agents Chemother

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(DNA) with a preference for A-T-rich sequences. Such an association causes a red shift in the absorption peak of distamycin and an increase in the melting temperature of native DNA (16)(17)(18).We attempted to correlate the biological activity of distamycin with its ability to interact with DNA by studying the effect of the drug on the transforming and transfective ability of bacterial and viral DNA in Bacillus subtilis. These two processes have a number of initial steps in common, namely binding and uptake of DNA by the cell. However, the final steps (integration into the host genome or production of phage DNA) are distinct. We thought that the similarities or differences between the sensitivity 1A. Minghetti was a Visiting Professor from the

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“…It suppresses the multiplication of T, and T, phages in E. coli K12 [2] and interferes with the multiplication of some DNA-viruses such as vaccinia, herpes simplex and adenoviruses [3] , Another interesting feature of distamycin A is its ability to prevent the induction of bacterial adaptive enzymes of E. coli [4,5].…”

Section: Introductionmentioning

confidence: 99%