Studies on the Application of Enzyme Immunoassays for the <i>Fusarium</i> Mycotoxins Deoxynivalenol, 3‐Acetyldeoxynivalenol, and Zearalenone

Usleber, Ewald; Renz, Volker; Märtlbauer, Erwin; Terplan, G.

doi:10.1111/j.1439-0450.1992.tb01213.x

Cited by 41 publications

(18 citation statements)

References 34 publications

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“…These assays were performed as previously described for FB 1 14 , AFB 1 3 , and DON and ZEA 13 . The microtitre plate wells (Maxisorp ® Nunc, Denmark), were coated with antimycotoxin antibody solution in 0.1M sodium bicarbonate buffer and incubated overnight at room temperature.…”

Section: Mycotoxin Analysismentioning

confidence: 99%

The Contamination of Kenyan Lager Beers with Fusarium Mycotoxins

Mbugua¹,

Gathumbi

2004

Journal of the Institute of Brewing

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Seventy five samples of two popular lager beers, namely Pilsner and Tusker were randomly collected from the city of Nairobi and the surrounding satellite towns in Kenya. The samples were analyzed for the presence of 4 mycotoxins, namely, deoxynivalenol (DON), fumonisin B 1 (FB 1 ), zearalenone (ZEA), and aflatoxin B 1 (AFB 1 ), by the competitive enzyme linked immunosorbent assay (ELISA) technique. The incidences of DON and ZEA were 100% in both brands, while for FB 1 the incidence was 72%, with incidences in Tusker (76.9%) being significantly higher than in Pilsner (66.7%) (p = 0.00). The mean values for contamination were 3.29 and 3.57 ng/mL for DON, 0.28 and 0.32 ng/mL for FB 1 and 7.84 and 8.50 pg/ml for ZEA in Tusker and Pilsner brands respectively. A positive occurrence association was found between DON and FB 1 and DON and ZEA, an indication of their common source from Fusarium sp. The results suggest low levels and safe exposure to consumers of Kenyan lager beers with Fusarium mycotoxins.

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Section: Mycotoxin Analysismentioning

confidence: 99%

The Contamination of Kenyan Lager Beers with Fusarium Mycotoxins

Mbugua¹,

Gathumbi

2004

Journal of the Institute of Brewing

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“…The capacity of the rumen microorganisms to degrade ZON was discussed as one reason for the widespread discrepancies between the single results of the studies (Prelusky et al, 1990). No residues or only traces were accordingly detected after administration of low ZON doses (Prelusky et al, 1990;Usleber et al, 1992;Goll et al, 1995). Furthermore, only little information is available on ZON residues in edible tissues.…”

Section: Deoxynivalenolmentioning

confidence: 99%

Relevance of the <i>Fusarium</i> toxins deoxynivalenol and zearalenone in ruminant nutrition. A review

Seeling¹,

Dänicke²

2005

J. Anim. Feed Sci.

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Deoxynivalenol (DON) and zearalenone (ZON) produced by Fusarium species are agriculturally important mycotoxins of relevance to livestock health. While ZON is known to cause oestrogenic syndromes in animals, a moderate ingestion of DON is associated with reduced performance and immune function. Among farm animals, ruminants appear to have a higher tolerance towards the effects of DON and ZON. As a consequence, feed producers may allocate cereals and roughages which appear contaminated with Fusarium toxins to ruminants. In combination with enhanced toxin concentrations during so-called Fusarium years, the possible effects in ruminants cannot be overlooked. However, only limited and inconsistent literature data are available about the effects of DON and ZON on ruminants. On the basis of the literature, the kinetics, biotransformation and carry over, as well as the effects of DON and ZON on ruminants, are reviewed. Furthermore, dosage and duration of toxin exposure as well as genetic and physiological factors of ruminants which could influence the variability of the toxin effects are considered and discussed. It is concluded that additional research is needed to study the effects of DON and ZON on ruminants, especially in lactating dairy cows.

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“…"*"* Cross-reactivity as determined in microtitre EIA by b Biermann and Terplan 1980;c Hack et al 1989;d Usleber et al 1991;" Usleber et al 1993a;f , Hack et al 1988;g Usleber et al 1992.…”

Section: Introductionmentioning

confidence: 99%

Multimycotoxin dipstick enzyme immunoassay applied to wheat

Schneider¹,

Usleber²,

Märtlbauer³

et al. 1995

Food Additives and Contaminants

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A membrane-based visual dipstick enzyme immunoassay for the simultaneous detection of up to five mycotoxins was developed. Multiple dots of the respective antibodies against aflatoxin B1 (AFB1), T-2 toxin (T-2), 3-acetyldeoxynivalenol (3-AcDON), roridin A (RA), and zearalenone (ZEA) were applied onto a dipstick membrane. The competitive immunoreactions were performed by incubation of the dipstick in a test tube containing sample solution and a mixture of the respective toxin-horseradish peroxidase conjugates. After a second incubation in enzyme substrate/chromogen solution, a complete suppression of the (blue) colour development of the respective dot was scored positive. Visual detection limits for AFB1, T-2, 3-AcDON, RA, and ZEA in buffer solution were 2 ng/ml, 5 ng/ml, 50 ng/ml, 30 ng/ml, and 5 ng/ml respectively. Using a simple extraction procedure, the detection limits of the multimycotoxin assay in artificially contaminated wheat samples were 30 ng/g, 100 ng/g, 600 ng/g, 500 ng/g, and 60 ng/g, respectively.

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Studies on the Application of Enzyme Immunoassays for the Fusarium Mycotoxins Deoxynivalenol, 3‐Acetyldeoxynivalenol, and Zearalenone

Cited by 41 publications

References 34 publications

The Contamination of Kenyan Lager Beers with Fusarium Mycotoxins

The Contamination of Kenyan Lager Beers with Fusarium Mycotoxins

Relevance of the <i>Fusarium</i> toxins deoxynivalenol and zearalenone in ruminant nutrition. A review

Multimycotoxin dipstick enzyme immunoassay applied to wheat

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