Studies on the biotransformation of novel disazo dyes by laccase

Soares, Graça M. B.; Amorim, Maria João; Hrdina, Radim; Costa‐Ferreira, Maria

doi:10.1016/s0032-9592(01)00244-8

Cited by 91 publications

(49 citation statements)

References 25 publications

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“…In the case of dye II, the presence of the coupling products of 1,2-naphthoquinone confirms the most accepted model of azo dye degradation by laccase (8,27).…”

Section: Discussionsupporting

confidence: 75%

Degradation of Azo Dyes by Trametes villosa Laccase over Long Periods of Oxidative Conditions

Zille

Górnacka

Rehorek

et al. 2005

Appl Environ Microbiol

153

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Trametes villosa laccase was used for direct azo dye degradation, and the reaction products that accumulated after 72 h of incubation were analyzed. Liquid chromatography-mass spectrometry (LC-MS) analysis showed the formation of phenolic compounds during the dye oxidation process as well as a large amount of polymerized products that retain azo group integrity. The amino-phenol reactions were also investigated by 13 C-nuclear magnetic resonance and LC-MS analysis, and the polymerization character of laccase was shown. This study highlights the fact that laccases polymerize the reaction products obtained during long-term batch decolorization processes with azo dyes. These polymerized products provide unacceptable color levels in effluents, limiting the application of laccases as bioremediation agents.The chemical structure of dyes is comprised of a conjugated system of double bonds and aromatic rings. The major classes of dyes have antroquinoid, indigoid, and azo aromatic structures. All of these structures allow strong -* transitions in the UV-visible (UV-Vis) area, with high extinction coefficients that allow us to consider these structures dye chromophores. Of all of these structures, the azo aromatic one is the most widespread dye class in the industry. The main drawback of this class of dyes is that they are not easily degraded by aerobic bacteria, and with the action of anaerobic or microaerobic reductive bacteria, they can form toxic and/or mutagenic compounds such as aromatic amines (9, 10, 34). There is a great environmental concern about the fate of these dyes, with special emphasis on reactive dyeing of cellulosic fibers, where large amounts of unbound dye are discharged in the effluent (24).Laccases have been extensively studied for their degradation of azo dyes (1,5,8,17,19,21,23,33). These enzymes are multicopper phenol oxidases that decolorize azo dyes through a highly nonspecific free radical mechanism forming phenolic compounds, thereby avoiding the formation of toxic aromatic amines (8,35). Over long periods of time, there can be a coupling between the reaction products, and even polymerization. It is known that laccases can catalyze the polymerization of various halogen-, alkyl-, and alkoxy-substituted anilines (15). In the literature, there is a large number of papers reporting on the decolorization of azo dyes; however, the fate of the products of azo dye laccase reactions and their possible polymerization have been ignored (7,16,25,28). Therefore, the purpose of this work was the study of azo dye degradation products in the presence of laccase. Direct azo dye laccase degradation and amino-phenol polymerization were performed for several days. The formed soluble products were studied by liquid chromatography-mass spectrometry (LC-MS), and the polymerized insoluble products were studied by 13 C-nuclear magnetic resonance ( 13 C-NMR). MATERIALS AND METHODSDyes, reagents, and enzymes. The investigated dyes, 3-(4-dimethylamino-1-phenylazo) benzenesulfonic acid (dye I) and 3-(2-hydroxy-1-naphthyl...

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“…In the case of dye II, the presence of the coupling products of 1,2-naphthoquinone confirms the most accepted model of azo dye degradation by laccase (8,27).…”

Section: Discussionsupporting

confidence: 75%

Degradation of Azo Dyes by Trametes villosa Laccase over Long Periods of Oxidative Conditions

Zille

Górnacka

Rehorek

et al. 2005

Appl Environ Microbiol

153

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“…They are characterized by the presence of at least one nitrogen-to-nitrogen double bond (-N=N-) bearing aromatic rings, and they dominate the worldwide market of dyestuff with a share of about 70 % (Soares et al 2002). Furthermore, some azo dyes and their dye precursors have been proven or are suspected to be human carcinogens as they generate toxic aromatic amines (Stylidi et al 2003).…”

Section: Introductionmentioning

confidence: 99%

Sonochemical removal of naphthol blue black azo dye: influence of parameters and effect of mineral ions

Dalhatou

Pétrier

Laminsi

et al. 2013

Int. J. Environ. Sci. Technol.

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Sonochemical degradation at 278 kHz of naphthol blue black (NBB, a diazo dye widely used in the textile and soap industries) has been investigated. The effects of different parameters and the influence of mineral matrix (bicarbonates ions and phosphates ions) on its sonodegradation have been evaluated. The influence of parameters linked to the pollutant (concentration: 0.50-97.32 lmol L -1 ), to the technique (power: 20-100 W) and to the natural medium (pH 3-10.8; bicarbonate ions; phosphate ions) has been studied. The decolourisation rate of NBB increases when substrate concentration or ultrasound power increases. Bicarbonate ions at natural medium concentration (2.97 mmol L -1 ) have a positive influence for low pollutant concentration (0.5-2.0 lmol L -1 ), due to carbonate radicals. Phosphate ions may also improve the kinetics, but this effect depends on the pH domains. Furthermore, acid pH (pH 3) has a positive effect for high pollutant concentration, whereas basic pH (pH 10.8) has a positive influence at low pollutant concentration.

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“…Azo dyes are the majority of all textile dyestuffs and are the most commonly synthetic dyes in the textile, printing and dyeing industries,because of their low biodegradability, some of these dyes have been reported to accumulate in surface water and sediments [1].As the largest type of dyes, azo dyes have differences structural based on one or more azo bonds (-N=N-) bearing aromatic ring sand consequently give out a great variety of colors [2], and dominates the worldwide market of dyestuffs with a share of about 70% [3].Approximately 10-15% of the azo dyes are released into the environment during manufacturing and usage, and poses a potential health hazard to all forms of life in river and lake Basin, majority of these dyes is either toxic or mutagenic and carcinogenic and threaten the life in much aspects in environment [4].…”

Section: Introductionmentioning

confidence: 99%

Decolorization and Degradation of Direct Blue 6 from textile wastewaters by Pseudomonas aeruginosa HS-DY012

Wu¹,

Cong²,

Pei³

et al. 2018

Proceedings of the 2017 3rd International Forum on Energy, Environment Science and Materials (IFEESM 2017)

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Abstract. Wastewater discharged from printing and dyeing plants on both sides of the Yangtze River seriously threatens the ecological and water security. Direct Blue 6 is a synthetic chemical dye and widely used in textile and dyeing industry. In this study, Pseudomonas aeruginosa HS-DY012 was used as a original strain in the decoloraziation of Direct Blue 6. Results indicated that HS-DY012 strain could completely decolorize and degrade 30 mg/L Direct Blue 6 dye in MSM medium within 18h culturing at 35 ℃ on a shaker at 120 rpm. The optimum condition of decolorization and degradation were determined, i.e. temperature at 35℃ and pH7.0, and the optimum concentration was 30 mg/L. HS-DY012 strain could effectually decolor seleted two type dyes both Reactive Black 5 and Congo red with a high degradation rate, as well as Acid scarled GR and Vat Yellow 2 could be decolored partially.These results indicated that HS-DY012 strain was a great degrader with a broad-spectrum dye decolorization character and it has a potential application.

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Studies on the biotransformation of novel disazo dyes by laccase

Cited by 91 publications

References 25 publications

Degradation of Azo Dyes by Trametes villosa Laccase over Long Periods of Oxidative Conditions

Degradation of Azo Dyes by Trametes villosa Laccase over Long Periods of Oxidative Conditions

Sonochemical removal of naphthol blue black azo dye: influence of parameters and effect of mineral ions

Decolorization and Degradation of Direct Blue 6 from textile wastewaters by Pseudomonas aeruginosa HS-DY012

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