Studies on the Capacity of Some Polysaccharides to Elicit Antibody Formation in Man

Allen, Peter Z.; Kabat, Elvin A.

doi:10.1084/jem.105.5.383

Cited by 29 publications

(11 citation statements)

References 30 publications

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“…and inulin in amounts up to 9/zg did not precipitate with antilevan C.S. Quantitative precipitin reactions with various fructosans and the antilevan from subject 1 DnL2 have been reported previously (20); with 1.5 ml of 1 D,3144 + 1 D8~I45 serum, taken 9 yr later, levan P6 precipitated 6/zg of antilevan N with 1/~g of !evan PG, giving 50% precipitation. Small amounts (1-5/zg) of perennial rye grass levan precipitated 0.5/zg of N while inulin failed to pre7 cipitate.…”

Section: Quantitative Precipitin Studies With Mouse Myeloma Proteins supporting

confidence: 57%

“…Human Antilevans.--Two human antilevan sera were studied. Antilevan from subject 1 (l Da3L24 + Ds4L25) (20) had been previously absorbed with insoluble blood group substance (35) and with Sephadex (13) to remove anti-A antibodies and antidextrans, respectively. Antilevan C.S.…”

Section: Materials Ana Methodsmentioning

confidence: 99%

“…Antilevan C.S. was prepared by immunization with levan P6 (20) and was kindly supplied by Doctors Valee Harisdangkul and Charles L. Christian, Cornell University Medical College, New York. Antigens.--The dextrans and levans used in this study have been described previously (20, 36 el.…”

Section: Materials Ana Methodsmentioning

confidence: 99%

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Immunochemical Studies on Mouse Myeloma Proteins Reactive With Dextrans or With Fructosans and on Human Antilevans

Cisar

Kabat

Liao

et al. 1974

The Journal of Experimental Medicine

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Four BALB/c IgA mouse myeloma proteins (W3129, W3434, QUPC 52, and UPC 102) reactive with dextran, four myeloma proteins reactive with fructosans, three IgA (W3082, UPC 61, and Y5476), and one IgG2a (UPC 10), and two human antilevans were studied immunochemically. Quantitative precipitin and inhibition assays showed that W3129, W3434, and QUPC 52 had specificities for isomaltose oligosaccharides similar to those previously found with α(1 → 6)-specific human antidextrans. W3129 and W3434 were most complementary to IM5 but W3129 reacted equally with IM4 and IM3 while W3434 had a greater affinity for IM4 than IM3. QUPC 52 had a larger combining region and was most complementary to IM6. Protein UPC 102 (IgA), like MOPC 104E (IgM) (27), was most complementary to the α(1 → 3)-linked trisaccharide, nigerotriose, and thus differed from J558 (29), which was inhibited best by nigeropentaose. UPC 102 was similar to J558 but they differed from MOPC 104E in their reactions with non-α(1 → 3)-linked disaccharides. The fructosan-specific myeloma proteins fell into two groups with different specificities. The first group, W3082 (IgA), UPC 61 (IgA), and the previously studied J606 (IgG3) (28, 29), reacted with inulin and W3082 and UPC 61 appeared to have identical specificities for ß(2 → 1)-linked fructofuranosyl residues with maximum complementarity for the tetrasaccharide ßDfructofuranosyl (2 → 1)ßDfructofuranosyl(2 → 1)ßDfructofuranosyl(2 → 6)Dglucose while protein J606 was inhibited best by the trisaccharide ßDfructofuranosyl(2 → 1)ßDfructofuranosyl(2 → 6)Dglucose. W3082 and UPC 61 also differed from J606 in their behavior toward sucrose and ßDfructofuranosyl(2 → 6)Dglucose as compared with αDglucosyl(1 → 3)Dfructose (turanose). The second group containing myeloma proteins UPC 10 (IgG2a) and Y5476 (IgA) behaved similarly to human antilevans in that neither reacted with inulin nor were they inhibited by the ß(2 → 1)-linked fructose oligosaccharides. Unlike the ß(2 → 1)-specific proteins, they reacted with perennial rye grass levan that contained over 90% ß(2 → 6)links. The differences in specificity and site size among homogeneous mouse myeloma proteins reactive with the same antigenic determinant are completely consistent with the concept that they represent products of homogeneous clones selected from the known heterogeneous population of antibody-forming cells.

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Section: Quantitative Precipitin Studies With Mouse Myeloma Proteins supporting

confidence: 57%

Section: Materials Ana Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Immunochemical Studies on Mouse Myeloma Proteins Reactive With Dextrans or With Fructosans and on Human Antilevans

Cisar

Kabat

Liao

et al. 1974

The Journal of Experimental Medicine

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“…Certain data with fructosan-reactive myeloma proteins (54,55) and human antilevans (55,84) are consistent with terminal and nonterminal specificities for levan chains . Proteins W3082 and UPC 61 which are probably identical and different from protein J606 (54,55,60) all have been shown to have,6 (2 -1) specificity but are thought to cross-react at the nonreducing ends of ,8 (2 -.…”

Section: Discussionmentioning

confidence: 56%

Binding properties of immunoglobulin combining sites specific for terminal or nonterminal antigenic determinants in dextran.

Cisar¹,

Kabat²,

Dörner³

et al. 1975

The Journal of Experimental Medicine

165

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Binding constants of the dextran-reactive BALB/c mouse IgA myeloma proteins W3129 and QUPC 52 have been determined for each member of the isomaltose series of oligosaccharides and for methyl alphaDglucoside. Protein W3129 has maximum complementarity for isomaltopentaose (IM5) deltaf degrees = 7,180 cal/mol) with 55-60% of the total binding energy directed against methylalphaDglucoside. Protein QUPC 52 gives maximum binding with isomaltohexaose (IM6) (deltaF degrees = -5,340 cal/mol) and has about 70% of its total binding energy for isomaltotriose (IM3), but at most only 5% for isomaltose (IM2) or methyl alphaDglucoside. Protein W3129 precipitates with branched dextrans high in alpha (1 yields 6) linkages and reacts with but does not precipitate a synthetic alpha (1 yields 6)-linked linear dextran. Protein QUPC 52 precipitates both branched and linear dextrans. Thus, the immunodominant group for protein W3129 is mimicked by methyl alphaDglucoside and this protein reacts exclusively at the terminal nonreducing ends of alpha (1 yields 6)-linked dextran chains. Protein QUPC 52 has an immunodominant group which is expressed by IM3 but not smaller oligosaccharides and this protein can react at nonterminal locations along alpha (1 yields 6)- linked dextran chains.Precipitation of linear dextran seems to be a valid although not quantitative assay for antidextrans with nonterminal specificity. Quantitative precipitin reactions with branched and linear dextrans suggest that alpha (1 yields 6)-specific human antidextrans are mixtures of molecules having terminal and nonterminal specificities and that the fraction of each type can vary among individuals. Rabbit antisera against IM3 or IM6 coupled to bovine serum albumin also appear to contain antibodies with nonterminal specificity for dextran chains although a large fraction has terminal specificity. Low molecular weight clinical dextran N-150N (congruent to 60,000) reacted more like linear dextran than like its parent native-branched dextran B512. This is thought to result from an abundance of nonterminal determinants in clinical dextran N-150N but a very small number of functional terminal determinants per molecule. An appreciation of terminal and nonterminal specificities and of the different immunodominant structures in isomaltosyl chains has proven to be of a great value in understanding the immunochemical reactions of dextrans. Moreover, certain previous findings with fructosan-reactive mouse myeloma proteins and human antilevans (55, 84) also suggest terminal and nonterminal specificities for levan chains.

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“…The high solubility and low viscosity of aqueous levan solutions would make it a suitable substitute for arabic gum [6]. Not only as a bioactive substance, levan, may also, have a wide range of applications in medicine, food, printing and cosmetics [7,8]. Several applications have been developed for levan, such as an anti-inflammatory agent against skin irritation and as a cell-proliferative agent [9].…”

Section: Introductionmentioning

confidence: 99%

Partial purification of a Bacillus licheniformis levansucrase producing levan with antitumor activity

Dahech¹,

Belghith²,

Belghith

et al. 2012

International Journal of Biological Macromolecules

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Studies on the Capacity of Some Polysaccharides to Elicit Antibody Formation in Man

Cited by 29 publications

References 30 publications

Immunochemical Studies on Mouse Myeloma Proteins Reactive With Dextrans or With Fructosans and on Human Antilevans

Immunochemical Studies on Mouse Myeloma Proteins Reactive With Dextrans or With Fructosans and on Human Antilevans

Binding properties of immunoglobulin combining sites specific for terminal or nonterminal antigenic determinants in dextran.

Partial purification of a Bacillus licheniformis levansucrase producing levan with antitumor activity

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