Studies on the cell membrane

Yamamoto, Hiroyuki; Masuda, Yasuske; Kuchii, Masato; Hayano, Taizo

doi:10.1254/fpj.68.142

Cited by 2 publications

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“…3) The present evidence that the time-course pattern of the enzyme activity was quite different between the sexes and the elevation of the activity was markedly enhanced by fasting, are quite comparable to those observed with NADH-cytochrome c reductase activity in the cell membrane fraction of liver (9,26).…”

Section: Discussionsupporting

confidence: 82%

“…It has been demonstrated by the authors that the increase of this enzyme activity in cell membrane preceded the change in microsomes at earlier stage of CCl4 poisoning, suggesting that the initial site of CCl4-induced liver damage is the cell membrane rather than the microsome (9,10). Accordingly, the present experiments were undertaken to clarify whether or not there is leakage of this enzyme into plasma from liver cells during the course of CCl4 poisoning, as is seen with GOT, GTP and other intracellular enzymes (11).…”

mentioning

confidence: 98%

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Studies on the Function of Cell Membrane

Yamamoto¹,

Kuchii²,

Masuda³

et al. 1973

Jpn.J.Pharmacol

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Abstract-NADH-cytochrome c reductase was not observed in normal rat plasma. NADH-cytochrome c reductase appeared in the plasma of rats after CCl4 administration. The time-course pattern of the enzyme activity was quite different between the sexes, and the elevation of the activity was markedly enhanced by fasting. NADH-cytochrome c reductase activity detected in the circulating plasma of CCl4-poisoned rats originated from the microsomal NADH-cytochrome b5 reductase system in liver cells. Mechanisms of the leakage of this enzyme into the plasma are discussed.Recent investigations concerning the mechanism of hepatotoxic action of CCl4 have demonstrated that the liver damage induced by CCl4 is due to direct action on this organ (1-3), and also endoplasmic reticulum is the primary site of CCl4-induced impairment (4-8), resulting from the evidence that the increase of NADH-cytochrome c reductase activity in microsomes was seen several hours after administration.It has been demonstrated by the authors that the increase of this enzyme activity in cell membrane preceded the change in microsomes at earlier stage of CCl4 poisoning, suggesting that the initial site of CCl4-induced liver damage is the cell membrane rather than the microsome (9, 10). Accordingly, the present experiments were undertaken to clarify whether or not there is leakage of this enzyme into plasma from liver cells during the course of CCl4 poisoning, as is seen with GOT, GTP and other intracellular enzymes (11).MATERIALS AND METHODS Male and female rats of Donryu S strain, weighing 150 to 250 g, were used. Some were fasted for 24 hr prior to CCl4 administration until the time of blood collection. Free access to water was permitted.CCl4 was dissolved in olive oil (1: 4, V/V) and administered i.p. in a dose of 0.2 ml CCl4 per kg body wt.Blood samples were obtained by heart puncture with heparinized syringe under pentobarbital anesthesia (30 mg/kg, i.p.), immediately cooled in chilled water and centrifuged

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Section: Discussionsupporting

confidence: 82%

mentioning

confidence: 98%