Studies on the Chromosomes of Mosquitoes1

Breland, Osmond P.

doi:10.1093/aesa/54.3.360

Cited by 41 publications

(14 citation statements)

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“…The cells' diploid chromosome number was 6, coinciding with that recorded for the species by Breland (1961). Based on direct measurements and the centromere position (C-bands), pair 1 was metacentric and of shortest length, whilst pairs 2 and 3 were slightly submetacentric and longer.…”

Section: A B C Dsupporting

confidence: 76%

A new continuous cell line from the mosquito Psorophora confinnis (Diptera: Culicidae) and its susceptibility to infections with some arboviruses

Bello

Rodrı́guez

Escovar

et al. 2001

Mem. Inst. Oswaldo Cruz

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Section: A B C Dsupporting

confidence: 76%

A new continuous cell line from the mosquito Psorophora confinnis (Diptera: Culicidae) and its susceptibility to infections with some arboviruses

Bello

Rodrı́guez

Escovar

et al. 2001

Mem. Inst. Oswaldo Cruz

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“…Micronuclei, also called satellites or extra-chromosomal elements, occur naturally in mosquitoes: Orthopodomyia and Toxorhynchites (Breland, 1961;Kitzmiller, 1963), Aedes atropalpus (Rai, 1963), A. vittatus (Rai, 1966), Psorophora signipennis (Mukherjee and Rees, 1970), and A. aegypti and A. fluviatilis treated with synthetic insecticides Bicudo, 1994, 1995; see also our Figure 3). Apparently micronuclei disappear after prophase due to chromosomal condensation in metaphase (Breland, 1961). However, micronuclei were found during telophase in Aedes dorsalis (Mukherjee and Rees, 1970).…”

Section: Discussionmentioning

confidence: 95%

Potential control of Aedes aegypti (Diptera: Culicidae) with Piper aduncum L. (Piperaceae) extracts demonstrated by chromosomal biomarkers and toxic effects on interphase nuclei

Wj²,

Jj³

et al. 2008

Genet. Mol. Res.

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ABSTRACT. Dillapiol, a phenylpropanoid isolate from essential oils of leaves of Piper aduncum (Piperaceae), has insecticidal, fungicidal and antimicrobial activities. The insecticidal activity of dil- Genotoxic effect of dillapiol on dengue mosquitoes lapiol was tested in vivo on the larvae and pupae of Aedes aegypti, the mosquito vector of dengue. Specifically, the effect of dillapiol on the formation of micronuclei and chromosome aberrations was analyzed. Dillapiol treatments comprised two concentrations of 200 and 400 μg/mL, dissolved in well water, and a pure well water control used to rear four generations of mosquitoes. Micronuclei occurred in mitotic diploid and tetraploid chromosomes of larvae; nuclear abnormalities also occurred in interphase, metaphase, telophase, and single nucleus cells of pupae. Mortality, oviposition, chromosome breakage, and anaphase bridges were significantly greater in the extract treatments than in controls. The genotoxic effects of dillapiol described here suggest that this natural product may be a useful alternative for the control of A. aegypti.

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“…Mid-forth instar larvae (65 hrs old) were used for preparing somatic chromo somes of the brain following the squash technique of Breland (1961) modified by French et al (1962). The karyotype studies were made by employing the modified mammalian chromosome technique of Lewis and Riles (1960) and chromosome measurements were made according to the procedure used by Baker and Aslamkhan (1969).…”

Section: Methodsmentioning

confidence: 99%

Cytogenetic Basis of Chemically-Induced Sterility in <i>Culex pipiens fatigans</i> Wiedemann

1973

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Studies on the potential use of induced sterility in insect control have resulted in the discovery of a large number of chemicals having effect on fertility and fecun dity of many species of insects. While the cumulative effect of these compounds lead to sterility their mode of action at cellular level is not yet clear. Many chemosterilants belonging to aziridinyl compounds, phosphoramides and s-tri azines are known to cause cytogenetic damage in the reproductive or embryonic tissues of insects (LaChance et al. 1968, Morgan 1967, Cline 1968, Reinecke et al. 1969). In addition, a similar damage to the brain cells of Aedes aegypti (L.) has been observed by larval treatment of apholate and hempa (Rai 1964, Sharma and Rai 1969, George and Brown 1967. As a result of these studies a concept has evolved that cytogenetic damage caused by chemicals is some how related to sterility (LaChance et a1. 1968).In our earlier studies, a number of alkylating and non-alkylating chemosteri lants were shown to induce sterility in Culex pipiens fatigans Wied. . The present investigations were, therefore, aimed to elucidate a cytogenetic basis of such chemosterilant-induced sterility in C. p. fatigans by detailed studies involving their effects on somatic, reproductive and embryonic tissues, and on DNA synthesis in the gonads. The present paper deals with the effects of apholate, metepa and hempa on the somatic chromosomes. Materials and methodsThe mosquitoes for the present studies were drawn from a Delhi strain of C. p. fatigans established in our laboratory since 1965 (Pillai and Grover 1969). The chemosterilants employed were as follows: 1) Apholate-2, 2, 4, 4, 6, 6-hexahydro-2, 2, 4, 4, 6, 6-hexakis (1-aziridinyl)-1, 3, 5, 2, 4, 6 triazatriphosphorine, 2) Metepa-tris (2-methyl-l-aziridinyl) phosphine oxide, and 3) Hempa-Hexamethyl phosphoric triamide.Mid-forth instar larvae (65 hrs old) were used for preparing somatic chromo somes of the brain following the squash technique of Breland (1961) modified by French et al. (1962). The karyotype studies were made by employing the modified mammalian chromosome technique of Lewis and Riles (1960) and chromosome measurements were made according to the procedure used by Baker and Aslamkhan (1969). Rapid cell divisions were found to occur in the brain cells of the larvae

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Studies on the Chromosomes of Mosquitoes1

Cited by 41 publications

References 0 publications

A new continuous cell line from the mosquito Psorophora confinnis (Diptera: Culicidae) and its susceptibility to infections with some arboviruses

A new continuous cell line from the mosquito Psorophora confinnis (Diptera: Culicidae) and its susceptibility to infections with some arboviruses

Potential control of Aedes aegypti (Diptera: Culicidae) with Piper aduncum L. (Piperaceae) extracts demonstrated by chromosomal biomarkers and toxic effects on interphase nuclei

Cytogenetic Basis of Chemically-Induced Sterility in <i>Culex pipiens fatigans</i> Wiedemann

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