Studies on the Chromosomes of Murine Dalton's Lymphoma Cells and the Effect of Cisplatin on Chromosomes of These Cells and Bone Marrow Cells in vivo.

Prasad, Surendra; Giri, Anirudha; Khynriam, Dimos

doi:10.1508/cytologia.63.405

Cited by 5 publications

(1 citation statement)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Sorsa et al (1985) reviewed the potential and real hazards due to occupational exposure to anticancer drugs, including cisplatin and reported it as a positive genotoxic agent in prokaryotes as well as in eukaryotes. Prasad et al (1998) studied the effect of cisplatin on the chromosomes of murine Dalton's lymphoma cells in in vivo bone marrow cells. Hannan (1988) made a comparative study of the cytotoxic and genotoxic effects of cisplatin and its analogue, TNO-6 in yeast.…”

Section: Introductionmentioning

confidence: 99%

Studies on the Genotoxic Effects of Anticancer Drug Carboplatin inin vivoMouse

Vijayalaxmi

D’Souza

2004

International Journal of Human Genetics

View full text Add to dashboard Cite

Genotoxicity of platinum antitumor agent carboplatin (Cbp) was investigated by using mouse in vivo test system. Three different doses of carboplatin viz., 30, 60 and 90 mg/kg b.w were used for the experiment. Fetal liver micronucleus (MN) test and sperm abnormality assay were used as the test parameters. Cyclophosphamide (50 and 100mg/kg b.w) was used as positive control and distilled water formed the negative control. Both the chemicals were administered intraperitoneally to experimental animals. MN and sperm preparations were made at 24 hr. and 35 days respectively. Carboplatin induced significant MN both in the fetal liver and the maternal bone marrow at different doses. It also induced high frequency of abnormal sperms at higher doses and the effect was significant at all the 3 doses tested.

show abstract

Section: Introductionmentioning

confidence: 99%

Studies on the Genotoxic Effects of Anticancer Drug Carboplatin inin vivoMouse

Vijayalaxmi

D’Souza

2004

International Journal of Human Genetics

View full text Add to dashboard Cite

show abstract

Untitled

Kharbangar

Khynriam

Prasad

2000

Cell Biology and Toxicology

View full text Add to dashboard Cite

Cisplatin treatment of tumor-bearing mice resulted a significant decrease of protein in the tissues studied (liver, kidney, and Dalton lymphoma) and also in their mitochondrial fractions. As compared to respective tissues, the protein decrease was noted to be more conspicuous in their mitochondrial fractions. Similarly, mitochondrial glutathione also decreased significantly in the tissues. However, succinate dehydrogenase activity was selectively decreased in the kidney and Dalton lymphoma cells, whereas in liver it remained almost unchanged. An increase in serum urea concentration and kidney mitochondrial lipid peroxidation was also observed after cisplatin treatment. It is suggested that the cisplatin-induced biochemical changes in mitochondria involving mitochondrial protein, glutathione, and succinate dehydrogenase could be the important potent cellular sites contributing to toxicity/cytotoxicity after cisplatin treatment.

show abstract

Untitled

Khynriam

Prasad

2001

Cell Biology and Toxicology

View full text Add to dashboard Cite

The involvement of glutathione, a major cellular antioxidant, in cisplatin-mediated development of various hematological changes in mice bearing ascites Dalton lymphoma tumor was investigated. With tumor growth, glutathione levels decreased in blood but increased in tumor cells. Cisplatin treatment of tumor-bearing mice caused a decrease in glutathione levels in blood, ascites supernatant, and tumor cells. Blood hemoglobin, erythrocytes, packed cell volume and leukocytes (eosinophils, basophils, and lymphocytes) were also decreased along with the development of various morphological abnormalities in erythrocytes (microcytes, macrocytes, echinocytes, acanthocytes, etc.) after cisplatin treatment. All these hematotoxic features were noted to be increased more when buthionine sulfoximine (a specific glutathione-depleting agent) was also given prior to cisplatin treatment. However, combination treatment of cysteine (precursor for glutathione synthesis) plus cisplatin resulted in an improvement in the glutathione levels and decrease in hematological toxicities. It is noted that the glutathione levels in blood and abnormalities in erythrocytes and other hematological parameters are inversely related in cisplatin-mediated cancer chemotherapy. It is suggested that blood glutathione may play an important role in the development of cisplatin-mediated hematological toxicity in the host.

show abstract

Studies on the Chromosomes of Murine Dalton's Lymphoma Cells and the Effect of Cisplatin on Chromosomes of These Cells and Bone Marrow Cells in vivo.

Cited by 5 publications

References 28 publications

Studies on the Genotoxic Effects of Anticancer Drug Carboplatin inin vivoMouse

Studies on the Genotoxic Effects of Anticancer Drug Carboplatin inin vivoMouse

Untitled

Untitled

Contact Info

Product

Resources

About