Studies on the Constituents of seeds of Pachyrrhizus erosus and Their Anti Herpes Simplex Virus (HSV) Activities.

Phrutivorapongkul, Ampai; Lipipun, Vimolmas; Ruangrungsi, Nijsiri; Watanabe, Tetsu; Ishikawa, Tsutomu

doi:10.1248/cpb.50.534

Cited by 30 publications

(26 citation statements)

References 8 publications

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“…Deoxycorticosterone[33] and flutamide[34] are corticosteroids and nonsteroidal antiandrogens, respectively. Pachyrrhizin[35] is extracted from a plant with unknown function.…”

Section: Resultsmentioning

confidence: 99%

An Image-Based Biosensor Assay Strategy to Screen for Modulators of the microRNA 21 Biogenesis Pathway

Shum

Bhinder²,

Radu³

et al. 2012

CCHTS

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microRNAs (miRNAs) are evolutionary conserved, small endogenous non-coding, RNA molecules. Although their mode of action has been extensively studied, little is known about their biogenesis. As their altered expression has been implicated in many diseases, small molecules that would modulate their expression are sought after. They are generated through the concerted action of several complexes which promote their transcription, maturation, export, trafficking, and loading of mature miRNA into silencing complexes. An increasing number of studies have suggested that each of these steps serves as a regulatory junction in the process, and therefore provides an intervention point. For this purpose, we have developed a simple image-based assay strategy to screen for such modulators. Here, we describe its successful implementation which combines the use of a microRNA 21 (miR-21) synthetic mimic together with an EGFP based reporter cell line, where its expression is under the control of miR-21, to monitor EGFP expression in a format suitable for HTS. The strategy was further validated using a small panel of known gene modulators of the miRNA pathway. A screen was performed in duplicate against a library of 6,912 compounds and identified 48 initial positives exhibiting enhanced EGFP fluorescence intensity. 42 compounds were found to be inherently fluorescent in the green channel leaving the remaining 6 as potential inhibitors and with a positive rate of 0.09%. Taken together, this validated strategy offers the opportunity to discover novel and specific inhibitors of the pathway through the screening of diverse chemical libraries.

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“…Deoxycorticosterone[33] and flutamide[34] are corticosteroids and nonsteroidal antiandrogens, respectively. Pachyrrhizin[35] is extracted from a plant with unknown function.…”

Section: Resultsmentioning

confidence: 99%

An Image-Based Biosensor Assay Strategy to Screen for Modulators of the microRNA 21 Biogenesis Pathway

Shum

Bhinder²,

Radu³

et al. 2012

CCHTS

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“…The majority of compounds found in the yam bean seeds belong to the isoflavonoid group, i.e., 3-arylcoumarin or phenylfuranocoumarin, coumestan, isoflavone, isoflavanone, and rotenoids. Compounds other than isoflavonoids were also found in the yam been seeds such as pachyrrhizid, saponins, and a dulcitol ( Phrutivorapongkul et al. 2002 , Barrera-Necha et al.…”

Section: Discussionmentioning

confidence: 99%

Oviposition Deterrent Activities of Pachyrhizus erosus Seed Extract and Other Natural Products on Plutella xylostella (Lepidoptera: Plutellidae)

Basukriadi

Wilkins

2014

Journal of Insect Science

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An extract of a rotenone-containing plant yam bean, Pachyrhizus erosus (L.) Urban, seeds was tested against the diamondback moth, Plutella xylostella (L.) in a greenhouse to determine its potential as an oviposition deterrent and compared with coumarin and rutin, known as diamondback moth oviposition deterrent compounds, rotenone, and an extract of Peruvian cube root, at a concentration of 0.5% (w/v). Oviposition deterrent index (ODI) was used to determine effects of extracts or compounds in inhibiting oviposition of diamondback moth. Coumarin showed a stronger deterrent effect than the yam bean seed extract with a higher ODI value. On the contrary, rotenone, rutin, and the cube root extract, containing 6.7% (w/w) of rotenone, showed no significant deterrent effects having low or negative ODI values, suggesting that the deterrent effect of the yam bean seed extract is not due to rotenone content of the yam bean seeds. The extract of yam bean seed and coumarin partially deterred the moth from laying eggs on treated leaves in a concentration-dependent manner. The effective concentration for 50% deterrency of coumarin and the yam bean seed extract were 0.11 and 0.83% (w/v), respectively. However, the yam bean seed extract showed a residual deterrent effect on the moth even at 3 d after the treatment and is probably because of its low volatile nature. A long-term deterrency of the yam bean seed extract is an advantage over coumarins. Both the yam bean seed extract and coumarin deterred diamondback moth from laying eggs in total darkness, indicating their nonvisual deterrent effect. This made the extract an effective deterrence to diamondback moth in light and in darkness. To conclude, this study revealed the potential of the crude extract of the yam bean seed to prevent diamondback moth from ovipositing on its plant host.

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“…A cis-fused tetrahydrochromeno [3,4-b]chromene core (B and C rings) is featured by quite a lot of rotenoid compounds ( Fig. 1), and a variety of biological and pharmacological properties including antibacterial, antiviral, antifungal, anticancer, antiplasmodial, anti-inflammatory, and insecticidal activities have been disclosed [4][5][6][7][8][9][10] . Particularly, 12ahydroxymunduserone (1a) shows efficient antitumor activity for HepG2 proliferation 11 ; tephrosin (1b) and deguelin (1e) are potent apoptotic and antiangiogenic reagents against various human cancer cells, such as lung, prostate, head and neck, and stomach cancer cells [12][13][14][15] ; retenone (1f) and 12ahydroxyrotenone (1d) have impressive inhibitory effect on breast cancer and lung cancer 16,17 ; tephrosin (1b), milletosin (1c), and rotenone (1f) are also important pesticides 18,19 .…”

mentioning

confidence: 99%

Asymmetric total synthesis of rotenoids via organocatalyzed dynamic kinetic resolution

et al. 2019

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Increasing effort has been made towards the asymmetric total synthesis of rotenoid natural products owing to their impressive biological and pharmaceutical activities. Here we report the modular asymmetric total synthesis of rotenoid natural products. The concise construction of the cis-fused tetrahydrochromeno[3,4-b]chromene core structure of rotenoids through N-heterocyclic carbene-catalyzed dynamic kinetic resolution is achieved, and a series of annulation products containing rotenoid key structures are rapidly assembled using this method. More importantly, the protocol enables the modular synthesis of a variety of rotenoid natural products in a highly convergent fashion, and the concise asymmetric total synthesis of tephrosin, the first asymmetric total synthesis of 12a-hydroxymunduserone, milletosin, and 12a-hydroxyrotenone, and the formal synthesis of deguelin are accomplished.

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Studies on the Constituents of seeds of Pachyrrhizus erosus and Their Anti Herpes Simplex Virus (HSV) Activities.

Cited by 30 publications

References 8 publications

An Image-Based Biosensor Assay Strategy to Screen for Modulators of the microRNA 21 Biogenesis Pathway

An Image-Based Biosensor Assay Strategy to Screen for Modulators of the microRNA 21 Biogenesis Pathway

Oviposition Deterrent Activities of Pachyrhizus erosus Seed Extract and Other Natural Products on Plutella xylostella (Lepidoptera: Plutellidae)

Asymmetric total synthesis of rotenoids via organocatalyzed dynamic kinetic resolution

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