From the methanolic extract of Cestrum diurnum L. leaves, a new 9-norlignan glucoside (cestrumoside) (1) was isolated in addition to six known glycosides (2-7). The structures of the isolated compounds were determined on the bases of NMR spectral analysis ( 1 H NMR, 13 C NMR, HSQC, HMBC, COSY) in addition to MS and CD to infer the stereochemistry of the compounds.