Studies on the decomposition of keratin wastes by saprotrophic microfungi. I. Criteria for evaluating keratinolytic activity

Korniłłowicz-Kowalska, Teresa

doi:10.5586/am.1997.006

Cited by 38 publications

(40 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Strain B21/4-5 which showed high keratinase activity and high loss of substrate mass was selected. As reported by Korniłłowicz-Kowalska (1997a), the loss of substrate mass is the most reliable indicator of keratinolytic activity of strains of keratinolytic fungi. Optimization conditions were designed based on two strains belonging to the genus Chrysosporium (Kushwaha 2000).…”

Section: Methodsmentioning

confidence: 75%

“…However, the release of ammonium ion was negatively correlated with caseinian-protease and keratinase activities, which may indicate that another enzyme takes part in the deamination. Products of protein proteolysis and amonification may inhibit proteolytic activity (Korniłłowicz-Kowalska 1997a). The release of proteins and peptides was especially dependent on keratinolytic activity.…”

Section: Resultsmentioning

confidence: 99%

“…The utilization of native keratin as the substrate is important for the assessment of keratinolytic abilities of microorganisms. Korniłłowicz-Kowalska (1997a) believes that this is measurable by the loss of substrate mass (native keratin). Kumar et al (2015) show that fungi of the genus Chrysosporium cause the loss of substrate (feather) mass ranging from 68 to 82%.…”

Section: Discussionmentioning

confidence: 99%

“…It contains 90% of protein, chiefly keratin, and from ~15–18% of nitrogen and 2–5% of sulfur (Kunert 2000; Korniłłowicz-Kowalska 1997a; Onifade et al 1998). Considerable amounts of this waste, such as feathers, bristles, horns and hooves and similar cornified skin products, are generated in animal raising and trading Korniłłowicz-Kowalska and Bohacz (2011).…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Biodegradation of feather waste keratin by a keratinolytic soil fungus of the genus Chrysosporium and statistical optimization of feather mass loss

Bohacz

2016

World J Microbiol Biotechnol

View full text Add to dashboard Cite

This paper assesses the ability of strains of Aphanoascus fulvescens and Chrysosporium articulatum isolated from soil (phaesol) to degrade native feather keratin. Strains were identified based on phenotypic traits and nucleotide sequencing. Response Surface Methodology was used to optimize cultivation conditions exhibiting the highest keratinolytic activity. The experiments were based on Box-Behnken designs for the loss of substrate mass (chicken feathers). While substrate mass loss is an “economic coefficient” that reliably indicates feather keratin degradation, it has not been studied before. Stationary liquid cultures of five selected strains were conducted in laboratory conditions at 28 °C using poultry feathers (1 g) as the sole source of carbon, nitrogen and energy. Enzymatic activities, keratin mineralization products and substrate mass loss were determined periodically. The mineralization of keratin proteins by strains yielded a high number of ammonium ions alkalinizing the medium. Increased ammonium ions inhibited the activity of caseinian protease and keratinase. A decrease in the concentration of these ions induced proteolytic enzymes, chiefly the activity of keratinase, at the end of fungal cultivation. Keratinase activity was related to protein- and peptide release and that of caseinian protease to sulfate ions. The highest loss of substrate mass in comparison to the reference strain CBS104.62 (35.4%) was recorded for Aphanoascus fulvescens B21/4-5 (65.9%). Based on a Box-Behnken design, the maximum loss of substrate mass for the Aphanoascus fulvescens strain (71.08%) can be achieved at pH 7.58 and temperature 28.7 °C.

show abstract

Section: Methodsmentioning

confidence: 75%

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Biodegradation of feather waste keratin by a keratinolytic soil fungus of the genus Chrysosporium and statistical optimization of feather mass loss

Bohacz

2016

World J Microbiol Biotechnol

View full text Add to dashboard Cite

show abstract

“…According to Korniłłowicz-Kowalska (1997), the most reliable indicator of microbial keratinolytic abilities is the mass loss of the keratin substrate. The keratinase activity, and the release of peptides, amino groups, amino acids, ammonia, sulfhydryl groups and sulfate are also other indicators of keratinolytic microorganisms skills (Korniłłowicz-Kowalska and Bohacz 2011).…”

Section: Discussionmentioning

confidence: 99%

Extracellular peptidases from Deinococcus radiodurans

et al. 2015

View full text Add to dashboard Cite

The extremophile Deinococcus radiodurans wild type R1 produces peptidases (metallo- and serine-) in TGY medium and in the media supplemented with human hair (HMY) and chicken feathers (FMY). Enzymatic screening on agar plates revealed peptidase activity. In TGY medium metallopeptidases were detected corresponding to a molecular mass range of 300-85 kDa (gelatinases); 280-130 (caseinases) and a 300 and a 170 kDa (keratinases); and a gelatinolytic serine peptidase (75 kDa). In HMY medium after 144 h, D. radiodurans produced keratinase (290 U/ml), gelatinase (619 U/ml) and sulfite (26 µg/ml). TGY medium produced higher proteolytic activity: 950 U/ml of gelatinolytic (24 h); 470 U/ml of keratinolytic (24 h) and 110 U/ml of caseinolytic (72 h). In the FMY medium, we found gelatinolytic (317 U/ml), keratinolytic (43 U/ml) and caseinolytic (85 U/ml) activities. The sulfite had a maximum release at 48 h (8.1 µg/ml). Enzymography analysis revealed that the keratinases degraded keratin after 24 h of reaction. The addition of sodium sulfite (1.0 %) improved the keratin degradation. Environmental Scanning Electron microscopy revealed alterations such as damage and holes in the hair fiber cuticle after D. radiodurans growth. This work presents for the first time D. radiodurans as a new keratinolytic microorganism.

show abstract

Changes in mineral forms of nitrogen and sulfur and enzymatic activities during composting of lignocellulosic waste and chicken feathers

Bohacz

2019

Environ Sci Pollut Res

View full text Add to dashboard Cite

The aim of this study was to show the dynamics of changes in the activity of enzymes responsible for C, N, and S metabolism, i.e., cellulase, protease, urease, and arylsulfatase in two lignocellulosic composts as well as changes in the concentration of mineral forms important in plant nutrition (N-NH 4 + , N-NO 3 − , S-SO 4 2− ). Most of the enzyme activity was higher during 10 weeks of composting in compost I, containing higher amounts of easily available organic matter than in compost II. Enzymatic activities in compost II remained at a higher level for a longer time, but they increased at a slower rate. Mineral content changes in the compost mass consisted primarily of an increase in N-NO 3 − concentration and a decrease in N-NH 4 + and S-SO 4 2− levels, especially in compost I. The concentration of mineral nitrogen and sulfur forms in compost water extracts was about 10–100 times lower than in the compost mass. At the end of composting, the amount of sulfates in the compost mass was 30 and 150 mg kg −1 dw in compost II and I, respectively. In this context, the composts obtained should be considered valuable for fertilizing soils poor in this component and for cultivating plants with high sulfate S demand.

show abstract

Studies on the decomposition of keratin wastes by saprotrophic microfungi. I. Criteria for evaluating keratinolytic activity

Abstract: The abilities and keratinolytic activity of saprotrophic microfungi (37 strains) have been investigated. Uniform criteria for the evaluation of this activity have been established.

Cited by 38 publications

References 0 publications

Biodegradation of feather waste keratin by a keratinolytic soil fungus of the genus Chrysosporium and statistical optimization of feather mass loss

Biodegradation of feather waste keratin by a keratinolytic soil fungus of the genus Chrysosporium and statistical optimization of feather mass loss

Extracellular peptidases from Deinococcus radiodurans

Changes in mineral forms of nitrogen and sulfur and enzymatic activities during composting of lignocellulosic waste and chicken feathers

Contact Info

Product

Resources

About