Studies on the Destruction of Red Blood Cells

Ham, Thomas Hale; Shen, Shu Chu; Fleming, Eleanor; Castle, W. E.

doi:10.1056/nejm194311042291901

Cited by 138 publications

(16 citation statements)

References 5 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Diffuse D.T.N. was similar to the appearance described in burned patients by Pack (1926), Shen et al (1943, Gibson (1944), and Goodpastor et al (1946). Essentially it had the same morphological pattern as Lucke's lower nephron nephrosis.…”

Section: Glomerular Capylay Thrombosissupporting

confidence: 83%

“…The histological pictures associated with acute renal failure following abortion, incompatible blood transfusion, crush injuries, severe trauma, blackwater fever, sulphonamide intoxication, and many other conditions including burns have been described by various writers (Bratton, 1941 ;Dunn, Gillespie, and Niven, 1941;Bywaters and Beall, 1941;Bywaters and Dible, 1942;Erb, Morgan, and Farmer, 1943;Shen, Ham, and Fleming, 1943;Lucke, 1946;Goodpastor, Levenson, Tagnon, Lund, and Taylor, 1946;Oliver, Mac-Dowell, and Tracy, 1951;Oliver, 1953;Bull and Dible, 1953). Lucke, whose report included 48 fatally burned subjects, claimed that there was a common morphological picture for acute renal failure caused by a variety of conditions.…”

mentioning

confidence: 99%

See 1 more Smart Citation

Distal Tubular and Proximal Tubular Necrosis in the Kidneys of Burned Patients

Sevitt¹

1956

Journal of Clinical Pathology

View full text Add to dashboard Cite

show abstract

Section: Glomerular Capylay Thrombosissupporting

confidence: 83%

mentioning

confidence: 99%

Distal Tubular and Proximal Tubular Necrosis in the Kidneys of Burned Patients

Sevitt¹

1956

Journal of Clinical Pathology

View full text Add to dashboard Cite

show abstract

“…If these compounds are produced by heating plasma then they should be present in the blood of burn patients, but their plasma does not cause hemolysis (Shen, Ham & Fleming, 1943). Nor does intravenous injection into dogs of plasma separated from heated dog whole blood cause hemolysis (Ham et al, 1948).…”

Section: Introductionmentioning

confidence: 99%

Thermal instability of red blood cell membrane bilayers: Temperature dependence of hemolysis

1988

View full text Add to dashboard Cite

Rates of human red blood cell hemolysis were measured as a function of temperature. Three distinct temperature intervals for hemolysis were noted: a) At temperatures equal to or less than 37 degrees C no hemolysis was observed for the duration of the incubation (30 hr). b) For temperatures exceeding 45 degrees C hemolysis rates are rapid and are accompanied by gross changes in cellular morphology. The activation energy for hemolysis is 80 kcal/mole; this value is characteristic of protein denaturation and enzyme inactivation suggesting that these processes contribute to hemolysis at these high temperatures. c) Between 38 and 45 degrees C the energy of activation is 29 kcal/mole, indicating that a fundamentally different process than protein inactivation is responsible for hemolysis at these relatively low temperatures. A mechanism based on the concept of the critical bilayer assembly temperature of cell membranes (N.L. Gershfeld, Biophys. J. 50:457-461, 1986) accounts for hemolysis at these relatively mild temperatures: The unilamellar state of the membrane is stable at 37 degrees C, but is transformed to a multibilayer when the temperature is raised; hemolysis results because formation of the multibilayer requires exposing lipid-free areas of the erythrocyte surface. An analysis of the activation energy for hemolysis is presented that is consistent with the proposed unilamellar-multibilayer transformation.

show abstract

“…At 50°C. fragmentation has begun or is in process (20,(26)(27)(28) and it would appear that as the new cells or fragments are being formed they are more fragile upon subjection to sonic oscillation. At 53°C.…”

Section: Discussionmentioning

confidence: 99%

Experimental Variations in Sonic Fragility of Red Cells

Guillet

Fowler

1954

Journal of General Physiology

View full text Add to dashboard Cite

The reaction of red cells to intense sonic and ultrssonic vibrations was studied by many workers following the development of the piezoelectric crystal and the magnetostriction oscillator. The original observers (1-10) attxibuted the hemolysis to rapid fluid movement as a result of cavitation. Slight physicalchemical changes in the cell were shown following subhemolyfic exposures to ultrasonic vibrations, such as a small inhibition of cell respiration (11), an increase in viscosity and a decrease in cell volume (12), a slight decrease in the oxygen-combining power at high oxygen pressures (13), and an unchanged resistance to hypotonic saline (14,15,12). The suspension medium has an effect upon the rate of hemolysis (10).Williams and Gaines (16) and Chambers and Gaines (17) using a son/c oscillator observed the laking of blood cells suspended in isotonic saline. By hemocytometer counts taken at regular intervals during the treatment, the following relationship was determined: N ~ Noe-~', N being the number of uncytolyzed cells at time, t, and No, the original number of cells. The hemolysis was explained as the result of rapid alterations of tension and compression produced in the surrounding medium by the vibrations.In the following experiments a further study of the kinetics of hemolysis by sonic oscillation was made. Also, the changes in sonic fragility brought about as a result of pretreatment of the cells were investigated. MethodBlood was drawn by venipuncture from normal healthy subjects and used immediately for experimentation. The anticoagulant used was a two-to-one mixture of ammonium and potassium oxalate. Hemolysis was induced by a Raytheon 50 watt, 9 kc. magnetostriction oscillator having a laminated nickel rod attached to the diaphragm at the base of a stainless steal cup. One cc. of blood was diinted with 25 cc. of saline, and this was delivered directly into the cup and subjected to sonic vibration for varying periods of time. The suspension was centrifuged following osciUafion and

show abstract

Studies on the Destruction of Red Blood Cells

Cited by 138 publications

References 5 publications

Distal Tubular and Proximal Tubular Necrosis in the Kidneys of Burned Patients

Distal Tubular and Proximal Tubular Necrosis in the Kidneys of Burned Patients

Thermal instability of red blood cell membrane bilayers: Temperature dependence of hemolysis

Experimental Variations in Sonic Fragility of Red Cells

Contact Info

Product

Resources

About