Studies on the distribution of immune cells in the uteri of prepubertal and cycling gilts

Bischof, Robert; Mr, Brandon; Cs, Lee

doi:10.1016/0165-0378(94)90035-3

Cited by 45 publications

(36 citation statements)

References 31 publications

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“…This finding is supported by previous reports in normally cyclic gilts in which uterine PMNs were commonly found during oestrus (Bischof et al, 1994 (Rozeboom et al, 1997). The impact of a PMN environment on embryo viability has not been investigated, but it is quite possible that the presence of PMNs when embryos enter the uterus at 2 days after ovulation is detrimental for normal development and viability (Hunter, 1990).…”

Section: Discussionsupporting

confidence: 79%

Characterization of uterine leukocyte infiltration in gilts after artificial insemination

Rozeboom¹,

Troedsson²,

Crabo³

1998

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Section: Discussionsupporting

confidence: 79%

Characterization of uterine leukocyte infiltration in gilts after artificial insemination

Rozeboom¹,

Troedsson²,

Crabo³

1998

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“…The mRNA expression of IL1B, IL6, and leukemia inhibitory factor were increased 2-3-fold in the endometrium after stimulation with seminal plasma (Gutsche et al 2003). These cytokines caused influx and accumulation of a variety of inflammatory leukocytes, macrophages, dendritic cells, and granulocytes to the endometrial stroma (Bischof et al 1994, Engelhardt et al 1997. There are reports suggesting that avian and mammalian cytokines may perform similar tasks, although their primary structures in most cases are remarkably different ( Weining et al 1998, Staeheli et al 2001.…”

Section: Sperm Survivability and Cytokines In Hen Oviductmentioning

confidence: 99%

Changes in the expression of interleukin-1β and lipopolysaccharide-induced TNF factor in the oviduct of laying hens in response to artificial insemination

Das¹,

Isobe²,

Yoshimura³

2009

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The aim of this study was to determine the physiological significance of interleukin-1b (IL1B) and lipopolysaccharide-induced TNF factor (LITAF) in the fate of sperm in the oviduct of laying hens after artificial insemination (AI ). Laying hens were inseminated with fresh semen, PBS or seminal plasma and tissues from different oviductal segments were collected to observe the general histology, changes in the mRNA expression of IL1B and LITAF and the localization of positive cells expressing immunoreactive IL1B (irIL1B). Semi-quantitative RT-PCR was used to observe the changes in mRNA expression of these molecules in the infundibulum, uterus, utero-vaginal junction (UVJ), and vagina after insemination. Intact sperm in the lumen and between the primary or secondary folds of the vagina were found until 6 h after insemination but were degraded at 12 h. The mRNA expression of IL1B and LITAF was significantly increased in the vagina until 6 h after AI but remained unchanged in the other oviductal segments. In the tissue of the vagina and UVJ, irIL1B was localized in the mucosal stroma. The number of irIL1B-positive cells was increased in the vagina but almost unchanged in UVJ after insemination with semen. Significant changes were not observed in the mRNA expression and irIL1B-positive cells in the vagina after PBS or seminal plasma insemination. The increase of IL1B and LITAF in the vagina may lead to sperm degradation and elimination by cilia of surface epithelium, whereas their lower levels in UVJ may permit sperm to survive in sperm storage tubules.

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“…Because of the random sources of animals and the unavailability of anamnesis vita, the stage of the swine estrous cycle of the extracted tissues was assessed visually by a determination of the ovarian follicle status and subsequently confirmed histologically (3,11,18). Histopathology.…”

Section: Methodsmentioning

confidence: 99%

Primary Cultures of Female Swine Genital Epithelial Cells In Vitro: a New Approach for the Study of Hormonal Modulation of Chlamydia Infection

et al. 2003

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Previous studies have demonstrated that female reproductive hormones influence chlamydial infection both in vivo and in vitro. Due to the reduced availability of human genital tissues for research purposes, an alternative hormone-responsive model system was sought to study chlamydial pathogenesis. Mature female swine eliminated from breeding programs were selected as the animals of choice because of the similarity of a sexually transmitted disease syndrome and sequelae in swine to a disease syndrome and sequelae found in humans, because of the near identity of a natural infectious chlamydial isolate from swine to Chlamydia trachomatis serovar D from humans, and because a pig's epithelial cell physiology and the mean length of its estrous cycle are similar to those in humans. Epithelial cells from the cervix, uterus, and horns of the uterus were isolated, cultivated in vitro in Dulbecco's minimum essential medium-Hanks' F-12 (DMEM-F-12) medium with and without exogenous hormone supplementation, and analyzed for Chlamydia suis S-45 infectivity. The distribution of chlamydial inclusions in swine epithelial cells was uneven and was influenced by the genital tract site and hormone status. This study confirmed that, like primary human endometrial epithelial cells, estrogen-dominant swine epithelial cells are more susceptible to chlamydial infection than are progesterone-dominant cells. Further, the more differentiated luminal epithelial cells were more susceptible to infection than were glandular epithelial cells. Interestingly, chlamydial growth in mature luminal epithelia was morphologically more active than in glandular epithelia, where persistent chlamydial forms predominated. Attempts to reprogram epithelial cell physiology and thereby susceptibility to chlamydial infection by reversestage, exogenous hormonal supplementation were unsuccessful. Freshly isolated primary pig epithelial cells frozen at ؊80°C in DMEM-F-12 medium with 10% dimethyl sulfoxide for several weeks can, after thawing, reform characteristic polarized monolayers in 3 to 5 days. Thus, primary swine genital epithelia cultured ex vivo appear to be an excellent cell model for dissecting the hormonal modulation of several aspects of chlamydial pathogenesis and infection.

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Studies on the distribution of immune cells in the uteri of prepubertal and cycling gilts

Cited by 45 publications

References 31 publications

Characterization of uterine leukocyte infiltration in gilts after artificial insemination

Characterization of uterine leukocyte infiltration in gilts after artificial insemination

Changes in the expression of interleukin-1β and lipopolysaccharide-induced TNF factor in the oviduct of laying hens in response to artificial insemination

Primary Cultures of Female Swine Genital Epithelial Cells In Vitro: a New Approach for the Study of Hormonal Modulation of Chlamydia Infection

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