Studies on the DNA binding and anticancer activity of Ru(ii) polypyridyl complexes by using a (2-(4-(diethoxymethyl)-¹H-imidazo[4,5-f][1,10] phenanthroline)) intercalative ligand

Abstract

Graphical representation of Ru(ii) complexes causing cell death.

On the Different Mode of Action of Au(I)/Ag(I)-NHC Bis-Anthracenyl Complexes Towards Selected Target Biomolecules

“…This indicates that the interaction between the gold complex and CT-DNA changes the elongation of the filament to a much lower extent than the silver one. Note that, under experimental conditions close to those used in the present viscometric experiments, the relative viscosity reaches values in the 1.6–2.0 range for Ethidium/DNA mixtures (DNA in base pairs) with ratios r b = 1.0 to 2.3 [ 53 , 54 , 55 ]. This confirms that the values obtained for the [Ag(EIA) 2 ] + complex are in line with those of a known intercalator.…”

Biological evaluation of a novel amino acid‐based macrocyclic Mn(III) and Fe(III) complexes

“…As the concentration of the complexes increases, the fluorescence intensity decreases. This clearly indicates that the EB molecules are replaced by the metal complexes in the DNA binding site . The fluorescence quenching spectra of DNA bound EB by complexes MnL and FeL are shown in (Figure S4a and S4b) .…”

Structure and cytotoxicity of zinc (II) and cobalt (II) complexes based on 1,3,5‐tris(1‐imidazolyl) benzene

“…The finding indicates a strong stacking interaction between the aromatic group and the base pairs of FS‐DNA, when the complexes intercalate to the FS‐DNA. This result shows a strong interaction between them in order to show the intensity between the compounds and FS‐DNA, the intrinsic binding constant K of the compounds with FS‐DNA are determined according to the following equation, [DNA]/( ε a − ε f ) [DNA]/( ε b − ε f ) + 1/ K ( ε b − ε f ), where [DNA] is the concentration of DNA in base pairs, ε a , ε f and ε b correspond to A obs /[complex], the extinction coefficient of the complex in the free complex, and the extinction coefficient of the complex in the fully bound form, respectively. The intrinsic binding constant K of complex 1 is calculated to be about 2.2 × 10 4 m −1 , complex 2 is 0.7 × 10 4 m −1 and complex 3 is 0.09 × 10 4 m −1 .…”

“…A classical intercalation binding needs more space of adjacent base pairs to accommodate the ligand and to extend the FS‐DNA helix, resulting in a significant increase in viscosity of FS‐DNA. On the other hand, non‐classical mode of interactions such as electrostatic or groove‐binding could bend the DNA helix, reduce its length, and may cause a reduction or no change in the DNA viscosity . Figure shows that as the concentration of the complex increases, the relative viscosity of the system increases, indicating that the complex binds to DNA in an intercalation mode.…”