Studies on the effect of inflammation on rat serum proteins

Abstract

Studies on the effect of inflammation on rat serum proteins. Can. J. Biochem. 48, 841-850 (1 970).The effect of turpentine-induced inflammation on rat serum proteins has been studied at short time periods of exposure to an inflammatory agent (5-96 h). There was an increase in protein-bound hexose and hexosamine of serum reaching a maximum at 48 h after administration of turpentine. Eight fractions were prepared from serum by a combination of chromatography on DEAE-cellulose and preparative electrophoresis on strips of gelatinized cellulose acetate. Most of the increase in protein-bound carbohydrate (77-86%) found in serum from experimental animals was located in three fractions following the fractionation procedure; immunological studies revealed the presence of fibrinogen and haptoglobin in two of these fractions. There was no evidence for the presence of a new protein in serum at short times of exposure to an inflanmatory agent. Proteins present in the perchloric acid soluble and seromucoid fractions of serum were found in five fractions, only two of which contributed to the increase in protein-bound carbohydrate of serum found as a result of inflammation. Studies involving incorporation of labelled precursors of glycoprotein biosynthesis into fractions from serum indicated that the most likely explanation for the increase in protein-bound carbohydrate of serum, as a result of inflammation, was an increase in the rate of synthesis of certain serum glycoproteins.

Studies on Acute Phase Proteins of Rat Serum. III. Site of Synthesis of Albumin and α1-Acid Glycoprotein and the Contents of These Proteins in Liver Microsome Fractions from Rats Suffering from Induced Inflammation

“…Protein was determined by the method of Lowry et al (12), but with modified reagents and volumes as described by Miller (13). Proteins were concentrated as previously described ( 1 ) .…”

Plasma protein response in experimental inflammation in the dog

“…It has been shown that in several proteins this is due to an increase in the rate of their production (Maung et al, 1968;Koj, 1968). In many investigations the plasma protein concentration has been determined indirectly from measurement of the amount of protein-bound hexose and hexosamine (Ashton et al, 1970). Most investigations with specific determination of several proteins have been carried out on man.…”

Characterization of Inhibitor(s) of Lymphocyte Activation in Serum from Rats with Adjuvant Arthritis

“…Some of these parameters have been used to describe the course of the disease and/or the effects of drugs on the syndrome. It appears, however, that most, if not all, of them reflect the inflammatory rather than the im munological aspects of the disease since similar modifications of serum components have been observed in rats suffering from non-immunologically mediated inflammatory reactions (7,8).…”