STUDIES ON THE FUNCTION OF CELL MEMBRANE 2ndREPORT: ELEVATION OF NADH-CYTOCHROME c REDUCTASE ACTIVITE IN THE LIVER CELL MEMBRANE OF RATS FOLLOWING CCl4 ADMINISTRATION

Masuda, Yasusuke; Kuchii, Masato; Yano, Ichiro; Yamamoto, Hiroyuki; Murano, Tadashi

doi:10.1254/jjp.23.627

Cited by 19 publications

(10 citation statements)

References 18 publications

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“…As reported previously (1), an elevation of NADH-cytochrome c reductase activity of cell membrane in male rats was not observed until 12 hr after CC1, administration.…”

Section: Discussionsupporting

confidence: 81%

Studies on the Function of Cell Membrane

Masuda¹,

Kuchii²,

Yamamoto³

et al. 1973

Jpn.J.Pharmacol

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It has been demonstrated in our papers (1, 2) that sex difference was observed not only in elevation of NADH-cytochrome c reductase activity in liver cell membranes of CCI,-administered rats, but also in leakage of the enzyme into circulating plasma. Possibility of participation of sex hormones in the elevation may be presumed, since androgens are well known to play an important role in the induction of drug metabolizing enzymes located in liver microsomes (3, 4). The present experiments were undertaken to verify such a participation in the eleva tion of NADH-cytochrome c reductase activity in liver plasma membrane of CCl4-ad ministered rats. MATERIALS AND METHODSMale and female rats of Donryu S strain weighing 180-200g were used.Under light ether anesthesia, orchiectomy and ovariectomy were performed while controls underwent a sham operation. After operation, all rats were given daily injec tions of penicillin for a period of one week to prevent post-operative suppuration, after which each operated group was subdivided into two groups.With orchiectomized rats, one group was given 17-a-methyltestosterone dissolved in sesame oil in a daily oral dose of 50 mg/kg for a period of one week and the second group was given 1.0 ml/kg of the dissolving oil. The former was confirmed at the end

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“…As reported previously (1), an elevation of NADH-cytochrome c reductase activity of cell membrane in male rats was not observed until 12 hr after CC1, administration.…”

Section: Discussionsupporting

confidence: 81%

Studies on the Function of Cell Membrane

Masuda¹,

Kuchii²,

Yamamoto³

et al. 1973

Jpn.J.Pharmacol

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“…Fractionation of liver plasma membranes and microsomes, determination of NADH cytochrome c reductase and NADH-ferricyanide dehydrogenase (NADH dehydrogenase) activities and protein assay were carried out using previously described methods (1 A concentration of DOC over 0.3% no longer caused an additional depression.…”

mentioning

confidence: 99%

Studies on the Function of Cell Membrane

Masuda¹,

Kuchii²,

Yamamoto³

et al. 1973

Jpn.J.Pharmacol

Self Cite

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Abstract-In liver microsomes of normal rats, both NADH-ferricyanide dehydro genase and NADH-cytochrome c reductase were almost completely solubilized by addition of 0.5 % DOC. Solubilization patterns were little affected by CCl₄ injection. In membranes, solubilization of dehydrogenase and reductase was less than that in microsomes. CCl₄ administration lowered the solubility of the dehydrogenase in membranes and increased three-fold the ratio of the reductase to the dehydrogenase remaining in the non-solubilized fraction. The reductase in membranes from CCI, treated rat liver was found to be more resistant to solubilization by DOC. The possibility of accumulation of NADH-cytochrome c reductase in plasma membranes after CCl₄ administration was discussed.

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“…Liver plasma membranes and microsomes were prepared according to the procedure de scribed in our previous papers (1,4). Fresh plasma membrane fractions were suspended in 150 mM KCl-25 mM Tris-HC1 buffer (pH 7.5) with high protein concentrations, kept in an ice bath and used within 12 hr after the isolation.…”

Section: Methodsmentioning

confidence: 99%