Studies on the Genetic Variability of Three Fish Species (Cyprinus carpio specularis, Cyprinus carpio communis and Oncorhynchus mykiss) Collected from Kashmir (India) Using Random Amplified Polymorphic DNA (RAPD) Technique

Abstract

The present study evaluated the patterns of morphometric and genetic variation using RAPD-PCR techniques for the first time on two species of Cyprinus and single species of Oncorhynchus mykiss from Srinagar, Kashmir, India. The Random Amplified Polymorphic DNA (RAPD) analysis was used to estimate the genetic relationships and diversity of three species of local fishes (Family Cyprinidae). A large numbers of highly informative DNA markers have been developed for the identification of genetic polymorphism. In the present study, genetic variability of three local fish species (Cyprinus carpio specularis, Cyprinus carpio communis and Oncorhynchus mykiss) collected from Dal Lake and local Trout Fish Farm (Harwan, Srinagar, India) was analyzed using the RAPD-PCR technique. Amplified DNA fragments with monomorphic profile were not found in the studied species, except for Cyprinus carpio specularis and Cyprinus carpio communis which presented monomorphic bands as both belong to the same genus. All species showed high levels of genetic variability among individuals. Cyprinus carpio communis and Cyprinus carpio specularis are more similar in comparison to the other Oncorhynchus mykiss based on morphological approach.

Molecular Identification of Grey Mullet species in the Mediterranean Sea of Egypt

“…However, it is important, to have an adequate knowledge on the genetic population structure of both the released stock and the wild population before carrying out Restocking and stock enhancement programs (Pereira et al, 2010). So, the genetic markers should be conducted to provide the information needed for a sound management of economic aquatic resources in wild fish stocks and/or farms (Saad et al, 2011) The number of reproducible and well-resolved bands analyzed in all populations examined in this study using the ten primers resulted in 175 polymorphic in Al Manzala 169 polymorphic in Bardawil and 121 polymorphic bands in Al Maadyeah, Hilal and Ali (2016) mentioned that the DNA markers were polymorphic reflecting a rich allelic diversity in the applied fish species. So, the primers of these loci are recommended to detect the genetic polymorphism and inferring the genetic variations for the applied fish species.…”