Studies on the Growth Habits and Characteristics of Two Polyploid Indica-Japonica Hybrid Rice with Powerful Heterosis

Song, Zhaoxia; Du, Changqing; Dai, Bingbing; Dong-ling, Chen; Chen, Jianguo; Cai, Detian

doi:10.1016/s1671-2927(07)60044-3

Cited by 14 publications

(15 citation statements)

References 2 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…High pollen fertility in autotetraploid F1 hybrids has been reported by Tu et al (2003) and Song et al (2006). We found that meiosis processed normally in autotetraploid F1 hybrids.…”

Section: Discussionsupporting

confidence: 83%

Cytogenetic studies on two F1 hybrids of autotetraploid rice varieties showing extremely high level of heterosis

Luan

Long

et al. 2007

Plant Syst. Evol.

View full text Add to dashboard Cite

Abstract. Mechanisms of two F1 hybrids (D46A · DTP-4 and D46A · Dminghui63) of autotetraploid rice (2n = 4x = 48) showing extremely high pollen fertility 87.40% and 85.97%, respectively, seed set 82.00% and 79.00%, respectively and extremely high level of heterosis were analyzed cytologically. The chromosome pairing of D46A · DTP-4 and D46A · Dminghui63 was normal at metaphase I(MI), and had almost no I or III, with an average of 0.020I +14.36 II 6.44rod+7.91ring) +0.01III + 4.80 IV + 0.01VIII and 0.06 I + 17.67 II (11.01rod + 6.67ring)] + 0.06 III +3.10IV+ 0.01VI, respectively. The most frequent chromosome configurations were 10II+7IV and 12II+ 6IV. The bivalent frequency was less frequent in hybrids than that in restoring parents, and the same results were gained from univalents, trivalent and multivalents. However, the quadrivalent frequency was significantly higher in hybrids than that in restoring parents at MI. The other meiotic phases progressed normally, except for low percentages of PMCs with lagging chromosomes at AI and low percentages of PMCs with micronuclei at telophaseI (TI) and telophaseII (TII). PMCs with lagging chromosomes at AI and PMCs with micronuclei at TI and TII showed negative correlation between pollen fertility and seed set. Above 90% of the PMCs could form normal microspores, which resulted in the production of viable pollen grains, abnormal microspores were observed including penta-fission and hexa-fission. Based on these results we suggest that the two F1 hybrids had better behaviors of chromosome pairing and genetic stability than autotetraploid rice and other autotetraploid plants ever studied.

show abstract

“…High pollen fertility in autotetraploid F1 hybrids has been reported by Tu et al (2003) and Song et al (2006). We found that meiosis processed normally in autotetraploid F1 hybrids.…”

Section: Discussionsupporting

confidence: 83%

Cytogenetic studies on two F1 hybrids of autotetraploid rice varieties showing extremely high level of heterosis

Luan

Long

et al. 2007

Plant Syst. Evol.

View full text Add to dashboard Cite

show abstract

“…In order to show this powerful heterosis more clearly and study the polyploid indica-jopanica hybrids more completely, two polyploid indica-japonica hybrids with powerful heterosis were cloned and planted in a big demonstration/study area. The results showed that the two tetraploid hybrids had normal growth and flowering habits and some characteristics similar to common diploid rice, and a high seed set rate (more than 84% [18] ). In 2005, more than 150 F 1 plants showed the characteristics of high seed set rate.…”

Section: Identification Of Polyploid Meiosis Stability (Pmes) and Higmentioning

confidence: 95%

“…Some hybrid combinations displayed powerful predominance. In 2003, for example, large-scale demonstration plantings of two polyploid indica-japonica hybrids showed this powerful heterosis [18] . In addition, hybrids made from PMeS lines and PA64s-4X (sterile lines of two-line hybrid rice) also had high seed set rates and obvious heterosis (Table 3).…”

Section: Summary Of Biology and Breeding Characters Of Pmes-1 And Pmes-2mentioning

confidence: 99%

“…After several years' efforts, two special polyploid rice lines, PMeS lines, had been bred by us. Because PMeS lines possess the special characteristics of stable meiosis and high seed set rates, both in the original lines and their hybrid offspring, the resulting polyploid indica-japonica hybrids all expressed powerful heterosis [18] . This fact proved not only that the successful selection of PMeS lines is a key factor, the effect of PMeS lines on polyploid rice breeding is the same as the effects of a half-dwarf gene on dwarf culms rice breeding, WA on three-line hybrid rice and NK58s on two-line hybrid rice.…”

Section: Polyploid Rice Breeding After the Use Of Pmes Lines Solves Tmentioning

confidence: 99%

See 1 more Smart Citation

The breeding of two polyploid rice lines with the characteristic of polyploid meiosis stability

Cai

Chen

et al. 2007

SCI CHINA SER C

View full text Add to dashboard Cite

Polyploidization is a basic feature of plant evolution. Nearly all of the main food, cotton and oil crops are polyploid. When ploidy levels increase, yields double; this phenomenon suggested a new strategy of rice breeding that utilizes wide crosses and polyploidization dual advantages to breed super rice. Because low seed set rates in polyploid rice usually makes it difficult to breed, the selection of Ph-liked gene lines was emphasized. After progenies of indica-japonica were identified and selected, two polyploid lines, PMeS-1 and PMeS-2 with Polyploid Meiosis Stability (PMeS) genes were bred. The procedure included seven steps: selecting parents, crossing or multiple crossing, back-crossing, doubling chromosomes, identifying the polyploid, and choosing plants with high seed set rates that can breed themselves into stable lines. The characteristics of PMeS were determined by observing meiotic behaviors and by cross-identification of seed sets. PMeS-1 and PMeS-2, (japonica rice), have several characteristics different from other polyploid rice lines, including a higher rate of seed set (more than 65%, increasing to more than 70% in their F1 offspring); and stable meiotic behaviors (pairing with bivalents and quarivalents nearly without over-quarivalent in prophase, nearly without lagging chromosomes in metaphase and without micronuclei in anaphase and telophase). The latter was obviously different from control polyploid line Dure-4X, which displayed abnormal meiotic behaviors including a higher rate of multivalents, univalents and trivalents in prophase, lagging chromosomes in metaphase and micronuclei in anaphase and telophase. There were also three differences of the breeding method between PMeS lines and normal diploid lines: chromosomes doubling, polyploidism identifying and higher seed set testing. The selection of PMeS lines is the first step in polyploid rice breeding; their use will advance the progress of polyploid rice breeding, which will in turn offer a new way to breed super rice.

show abstract

“…The Balilla-4X line without the PMeS background had a low seed set rate with disorganized meiosis behavior, and was applied as CK. The tetraploid rice lines were cultured according to our patent and by following a previously published technique (Cai et al 2004b;Song et al 2006).…”

Section: Methodsmentioning

confidence: 99%

Genome duplication effects on pollen development and the interrelated physiological substances in tetraploid rice with polyploid meiosis stability

Zhang

Wei

et al. 2010

Planta

View full text Add to dashboard Cite

The breeding of polyploid rice made no breakthrough for a long time because of low seed set. The discovery and application of polyploid meiosis stability (PMeS) material played a pivotal role in solving this problem. Our results indicated that genome duplication led to different outcomes in different rice cultivars in terms of pollen fertility, viability, and the accumulation of important physiological substances such as free proline and endogenous hormones. Pollen from the PMeS HN2026-4X lines showed a high fertility and viability similar to those of HN2026-2X (4X indicates tetraploid while 2X indicates the diploid), whereas both rates decreased dramatically in Balilla-4X. The results of pollen microstructure and ultrastructure investigations suggested that the pollen development pattern in HN2026-4X appeared normal at all stages, but a lot of changes were discovered in Balilla-4X. Stable meiosis, timely tapetum degradation, and normal mitochondria development were critical factors insuring the high frequency pollen fertility of PMeS rice. The free proline content increased markedly in HN2026-4X as compared to HN2026-2X, but it was decreased for Balilla-4X. Genome duplication effects on regulating endogenous hormones accumulation in pollen were evident, resulting in the clear difference between PMeS HN2026-4X and Balilla-4X. The accumulation of IAA, ZR, and GA in mature pollen distinguished HN2026-4X from Balilla-4X, which was linked to normal pollen development. In particular, the excessive accumulation of ABA at the meiosis stage may be correlated to disorganized meiosis in Balilla-4X. All the results provided unequivocal evidence that genome duplication played specific roles in the normal pollen development of PMeS HN2026-4X.

show abstract

Studies on the Growth Habits and Characteristics of Two Polyploid Indica-Japonica Hybrid Rice with Powerful Heterosis

Cited by 14 publications

References 2 publications

Cytogenetic studies on two F1 hybrids of autotetraploid rice varieties showing extremely high level of heterosis

Cytogenetic studies on two F1 hybrids of autotetraploid rice varieties showing extremely high level of heterosis

The breeding of two polyploid rice lines with the characteristic of polyploid meiosis stability

Genome duplication effects on pollen development and the interrelated physiological substances in tetraploid rice with polyploid meiosis stability

Contact Info

Product

Resources

About