Studies on the host range of the barley strain of <i>Wheat dwarf virus</i> using an agroinfectious viral clone

Ramsell, J. N. E.; Boulton, Margaret I.; Martin, Darren P.; Valkonen, Jari P. T.; Kvarnheden, Anders

doi:10.1111/j.1365-3059.2009.02146.x

Cited by 22 publications

(25 citation statements)

References 44 publications

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“…For example, whereas the ''A'' strain of MSV is clearly the only group of MSV variants that cause severe disease in maize [37,58], the ''A'' strain of WDV preferentially infects barley, whereas the ''C'' strain preferentially infects wheat [50].…”

Section: Rational Mastrevirus Species and Strain Demarcation Criteriamentioning

confidence: 99%

A genome-wide pairwise-identity-based proposal for the classification of viruses in the genus Mastrevirus (family Geminiviridae)

et al. 2013

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Recent advances in the ease with which the genomes of small circular single-stranded DNA viruses can be amplified, cloned, and sequenced have greatly accelerated the rate at which full genome sequences of mastreviruses (family Geminiviridae, genus Mastrevirus) are being deposited in public sequence databases. Although guidelines currently exist for species-level classification of newly determined, complete mastrevirus genome sequences, these are difficult to apply to large sequence datasets and are permissive enough that, effectively, a high degree of leeway exists for the proposal of new species and strains.The lack of a standardised and rigorous method for testing whether a new genome sequence deserves such a classification is resulting in increasing numbers of questionable mastrevirus species proposals. Importantly, the recommended sequence alignment and pairwise identity calculation protocols of the current guidelines could easily be modified to make the classification of newly determined mastrevirus genome sequences significantly more objective. Here, we propose modified versions of these protocols that should substantially minimise the degree of classification inconsistency that is permissible under the current system. To facilitate the objective application of these guidelines for mastrevirus species demarcation, we additionally present a user-friendly computer program, SDT (species demarcation tool), for calculating and graphically 123Arch Virol (2013) 158:1411-1424 DOI 10.1007/s00705-012-1601 displaying pairwise genome identity scores. We apply SDT to the 939 full genome sequences of mastreviruses that were publically available in May 2012, and based on the distribution of pairwise identity scores yielded by our protocol, we propose mastrevirus species and strain demarcation thresholds of [78 % and [94 % identity, respectively.

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Section: Rational Mastrevirus Species and Strain Demarcation Criteriamentioning

confidence: 99%

A genome-wide pairwise-identity-based proposal for the classification of viruses in the genus Mastrevirus (family Geminiviridae)

et al. 2013

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“…A great emphasis has been emerged here stressed on the biological difference between the viral isolates would be classified into different strains. For example, whereas the ''A'' strain of MSV is clearly the only group of MSV variants that cause severe disease in maize [28,42], the ''A'' strain of WDV favourably infects barley, whereas the ''C'' strain preferentially infects wheat [39], our Egyptian isolate of CpCDV could be assigned as a specific strain preferentially infects squash which is essentially a non-host species of this virus. Through recombination analysis performed using RDP4, it has been observed that the Egyptian isolate although more closely related to the Syrian isolate but a considerable part of the genome found to be originated from a different isolate of BeYDV assigned in the genbank under accession no ENA|DQ458791 (Fig.…”

Section: Discussionmentioning

confidence: 99%

First genome analysis and molecular characterization of Chickpea chlorotic dwarf virus Egyptian isolate infecting squash

2015

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This study aims to identifying and characterizing some molecular properties of geminiviruses co-infection in squash field crop cultivated in Egypt. Squash crops observed to be heavily infected with several insect vectors, also severe chlorosis and stunting was observed. Electron microscopic analysis has revealed geminate capsid particles which indicate the infection of Geminiviruses, especially SqLCV which represent an economic problem to squash filed crop in Egypt. We have investigated possible mixed infections with different plant viruses associated with chlorotic stunt diseases and or other genus groups of geminiviruses. The main objective of this study is to investigate the recombination events, possible recombinants and variants among these genera in the same family differing in vector transmission. This is the first report of the molecular characterization, phylogenetic analysis and putative recombination events of the full length genome of the Chickpea Chlorotic Dwarf Mastrevirus in Egypt. And the first report of co-infection with another begomovirus infecting squash plants. A full length clone of both viruses were isolated and characterized at the molecular level. The complete nucleotide sequence of DNA-A was determined (2,572 bp) and submitted to the genbank under accession no. KF692356. The isolate from Egypt has about 97.8 % homology with the Chickpea chlorotic dwarf virus (CpCDV) isolate from Syria DNA-A isolate FR687959, a 83.2 % homology with the Sudan isolate AM933134 and a 82.7 % homology with Pakistan isolate FR687960. To best of our knowledge this is the first report of complete genome of CpCDV that infect squash plants in Egypt and worldwide.

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“…Transgenic and control plantlets were used for virus infection studies. To mimic the real field infection process, WDV-infected barley plants were produced by Agrobacterium-mediated delivery of the agroinfectious WDV clone into barley (Ramsell et al, 2009). Then, the generated WDVinfected plants were used to feed the collected and propagated leafhoppers.…”

Section: Transgenic Barley Lines and Resistance Phenotyping Followingmentioning

confidence: 99%

“…The virion-sense V1 and V2 open reading frames (ORFs) encode the viral movement protein (MP) and coat protein (CP), whereas the other two ORFs, C1 and C2, located on complementary-sense, encode Rep and RepA proteins, respectively, associated with virus replication (Boulton, 2002). WDV is a pathogen of both wheat and barley, associated with chlorosis, severe stunting and even plant decline, causing severe yield losses in many countries (Ramsell et al, 2009). There are two main types of WDV strain, barley and wheat strains, sharing approximately 84% nucleotide identity, which are able to infect the other host with low efficiency (Koklu et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

Polycistronic artificial miRNA‐mediated resistance to Wheat dwarf virus in barley is highly efficient at low temperature

Kis

Tholt

Ivanics

et al. 2015

Molecular Plant Pathology

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Infection of Wheat dwarf virus (WDV) strains on barley results in dwarf disease, imposing severe economic losses on crop production. As the natural resistance resources against this virus are limited, it is imperative to elaborate a biotechnological approach that will provide effective and safe immunity to a wide range of WDV strains. Because vector insect-mediated WDV infection occurs during cool periods in nature, it is important to identify a technology which is effective at lower temperature. In this study, we designed artificial microRNAs (amiRNAs) using a barley miRNA precursor backbone, which target different conservative sequence elements of the WDV strains. Potential amiRNA sequences were selected to minimize the off-target effects and were tested in a transient sensor system in order to select the most effective constructs at low temperature. On the basis of the data obtained, a polycistronic amiRNA precursor construct (VirusBuster171) was built expressing three amiRNAs simultaneously. The construct was transformed into barley under the control of a constitutive promoter. The transgenic lines were kept at 12-15 °C to mimic autumn and spring conditions in which major WDV infection and accumulation take place. We were able to establish a stable barley transgenic line displaying resistance to insect-mediated WDV infection. Our study demonstrates that amiRNA technology can be an efficient tool for the introduction of highly efficient resistance in barley against a DNA virus belonging to the Geminiviridae family, and this resistance is effective at low temperature where the natural insect vector mediates the infection process.

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Studies on the host range of the barley strain of Wheat dwarf virus using an agroinfectious viral clone

Cited by 22 publications

References 44 publications

A genome-wide pairwise-identity-based proposal for the classification of viruses in the genus Mastrevirus (family Geminiviridae)

A genome-wide pairwise-identity-based proposal for the classification of viruses in the genus Mastrevirus (family Geminiviridae)

First genome analysis and molecular characterization of Chickpea chlorotic dwarf virus Egyptian isolate infecting squash

Polycistronic artificial miRNA‐mediated resistance to Wheat dwarf virus in barley is highly efficient at low temperature

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