Studies on the HPRT mutant frequency in T lymphocytes from healthy Indian male population as a function of age and smoking

SummaryTo determine the relationship between aging, cell proliferation and mutation in different cell types, hearts, brains and kidneys from G11 PLAP mice between 1 week and 24 months of age were examined. Mutant cells were Regardless of tissue and cell type, PLAP + cells occurred as singletons and in clusters, both of which increased in frequency with age. These data show that age-associated accumulation of mutant cells occurs in diverse cell types and is due to both new mutation and proliferation of mutant cells, even in cell types that tend to not proliferate.

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Dose-Rate Effect on the Induction ofHPRTMutants in Human G₀Lymphocytes ExposedIn Vitroto Gamma Radiation

Kumar¹,

Mohankumar²,

Hamza³

et al. 2006

Radiation Research

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The influence of dose rate on expression time, cell survival and mutant frequency at the hypoxanthine-guanine phosphoribosyltransferase (HPRT) locus was evaluated in human G(0) peripheral blood lymphocytes exposed in vitro to gamma rays at low (0.0014 Gy/min) and high (0.85 Gy/min) dose rates. A cloning assay performed on different days of postirradiation incubation indicated an 8-day maximum expression period for the induction of HPRT mutants at both high and low dose rates. Cell survival increased markedly with decreasing dose rate, yielding D(0) values of 3.04 Gy and 1.3 Gy at low and high dose rates, respectively. The D(0) of 3.04 Gy obtained at low dose rate could be attributed to the repair of sublethal DNA damage taking place during prolonged exposure to low-LET radiation. Regression analysis of the mutant frequency yielded slopes of 12.35 x 10(-6) and 3.66 x 10(-6) mutants per gray at high and low dose rate, respectively. A dose and dose-rate effectiveness factor of 3.4 indicated a marked dose-rate effect on the induced HPRT mutant frequency. The results indicate that information obtained from in vitro measurements of dose-rate effects in human G(0) lymphocytes may be a useful parameter for risk estimation in radiation protection.

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Studies on the HPRT mutant frequency in T lymphocytes from healthy Indian male population as a function of age and smoking

Cited by 4 publications

References 41 publications

The L84F polymorphism in the O 6 -Methylguanine-DNA-Methyltransferase (MGMT) gene is associated with increased hypoxanthine phosphoribosyltransferase (HPRT) mutant frequency in lymphocytes of tobacco smokers

The L84F polymorphism in the O 6 -Methylguanine-DNA-Methyltransferase (MGMT) gene is associated with increased hypoxanthine phosphoribosyltransferase (HPRT) mutant frequency in lymphocytes of tobacco smokers

Mutation in aging mice occurs in diverse cell types that proliferate postmutation

Dose-Rate Effect on the Induction ofHPRTMutants in Human G₀Lymphocytes ExposedIn Vitroto Gamma Radiation

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Studies on the HPRT mutant frequency in T lymphocytes from healthy Indian male population as a function of age and smoking

Cited by 4 publications

References 41 publications

The L84F polymorphism in the O 6 -Methylguanine-DNA-Methyltransferase (MGMT) gene is associated with increased hypoxanthine phosphoribosyltransferase (HPRT) mutant frequency in lymphocytes of tobacco smokers

The L84F polymorphism in the O 6 -Methylguanine-DNA-Methyltransferase (MGMT) gene is associated with increased hypoxanthine phosphoribosyltransferase (HPRT) mutant frequency in lymphocytes of tobacco smokers

Mutation in aging mice occurs in diverse cell types that proliferate postmutation

Dose-Rate Effect on the Induction ofHPRTMutants in Human G0Lymphocytes ExposedIn Vitroto Gamma Radiation

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Dose-Rate Effect on the Induction ofHPRTMutants in Human G₀Lymphocytes ExposedIn Vitroto Gamma Radiation