Studies on the Interaction Between Coxsackievirus A9 and Hela Cells

Dextran sulfate aggregates several enteroviruses depending not only on the pH, the ionic strength of the medium, but also on the protein content of the fluids and on strain specificities of the viruses. The aggregation effect was measured by filtration experiments, by sedimentation in the ultracentrifuge and by electron microscopy. The well known inhibiting effect of dextran sulfate on plaque formation may be due to its aggregating effect: A very strong inhibition of the release of matured virions from the infected cells is observed in medium containing dextran sulfate, whereas the adsorption process is inhibited much less compared with PBS controls. The maximal effect on virus aggregation, plaque size and virus release is observed at the same concentration of dextran sulfate.

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Strain-specific aggregation of enterovirus by dextran sulfate

Totsuka

Mukoyama

Tagaya

1981

Archives of Virology

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Variation in susceptibility of HeLa cell lines to coxsackievirus A 9

Matsumoto

Mukai

Tagaya

1979

Archives of Virology

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In the course of serial passages for several years a line of uncloned HeLa cells (A line) showed a gradual decrease in plaquing efficiency by coxsackievirus A9 (CA9 virus), while subcultures prepared from the same line kept frozen at an early passage level (A original line) did not show any change. However, it was observed later that the plaque-forming ability of the A original line (A orig. line) also decreased after serial passages as was observed with the A line. Comparing the characteristics of the same cell line at two different passage levels, it was found that the efficiency of adsorption of virus to cells was nearly the same, while virus yield at 8 hours after infection was different. The activity of alkaline phosphatase of cells was also different between these two passage levels, suggesting that a high enzymatic activity is associated with the susceptibility of cell cultures to CA9 virus. Magnesium chloride at 25 mM enhanced plaque formation by CA9 virus in highly passaged less susceptible cell cultures, and a possible role of the chemical as a stabilizer of alkaline phosphatase was discussed.

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Studies on the Interaction Between Coxsackievirus A9 and Hela Cells

Matsumoto¹,

Nakano

Nishikawa³

et al. 1976

JJMSB

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SUMMARY:Most of the coxsackievirus A9 (CA 9 virus) including the prototype strain formed plaques in HeLa cell monolayers under agar overlay, although they showed little or no cytopathogenicity under fluid medium. These viruses were isolated or passaged in primary cynomolgus monkey kidney (MK) cell cultures, and the infectivity of any strain in terms of plaque-forming units was much higher in MK cells than in HeLa cells, even after plaque purification of the virus in HeLa cell cultures. CA 9 virus contained in the original throat swabs as well as some clones obtained by plaque purification in MK cells failed to form plaques in HeLa cells, but virus preparations obtained after several undiluted passages through MK cells included plaque-formers in HeLa cells, suggesting that such plaque (HeLa)-forming viruses may have developed at a certain rate during multiplication of the original non-plaque (HeLa)-forming virus population in MK cells.Out of four lines of HeLa cells examined, two, including a clonal line 83, failed to support plaque formation by CA 9 virus.

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Studies on the Interaction Between Coxsackievirus A9 and Hela Cells

Cited by 4 publications

References 9 publications

Strain-specific aggregation of enterovirus by dextran sulfate

Strain-specific aggregation of enterovirus by dextran sulfate

Variation in susceptibility of HeLa cell lines to coxsackievirus A 9

Studies on the Interaction Between Coxsackievirus A9 and Hela Cells

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