Studies on the Life History of Eurytrema pancreaticum Janson, 1889

Tang, Chufei

doi:10.2307/3273289

Cited by 26 publications

(14 citation statements)

References 3 publications

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“…is a fluke that parasitizes the pancreatic ducts and rarely the bile ducts of cattle, buffaloes, camels, sheep, and goats (BASCH, 1966;SOULSBY, 1982;ISHII et al, 1983;MATTOS JUNIOR;VIANNA, 1987). Parasitism frequently results in epithelial hyperplasia, hypertrophy of pancreatic ducts, and periductal fibrosis that lead to eurytrematosis (TANG, 1950;MATTOS JUNIOR;VIANNA, 1987;HEADLEY et al, 2009). Although bovine eurytrematosis is more frequently associated with Eurytrema coelomaticum in Brazil (TRAVASSOS et al, 1969), E. pancreaticum has been described in studies that have not examined the morphological characteristics of the trematode (CAMPOS et al, 1974;BUSETTI et al, 1983).…”

Section: Introductionmentioning

confidence: 99%

Molecular characterization of Eurytrema coelomaticum in cattle from Paraná, Brazil

Figueira

Oliveira

Taroda

et al. 2014

Rev. Bras. Parasitol. Vet.

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This study investigated the occurrence of Eurytrema spp. in cattle by analysis of the partial 18S rRNA gene sequence. Trematodes from 44 bovine pancreas were collected and classified based on typical morphological features. PCR assay and sequence analyses of amplified products confirmed that the trematodes classified as Eurytrema coelomaticum were phylogenetically distinct from those identified as E. pancreaticum. The results of this study represent the first molecular characterization of E. coelomaticum within the Americas, and provide an efficient method to differentiate digenean trematodes of domestic animals.Keywords: Bovine trematode, Eurytrema spp., PCR, phylogeny. ResumoEste estudo avaliou a ocorrência de Eurytrema spp. em bovinos por meio da análise da sequência parcial do gene 18S rRNA. Trematódeos procedentes de 44 pâncreas bovinos foram coletados e classificados com base em aspectos morfológicos típicos. A técnica de PCR e a análise da sequência de nucleotídeos dos produtos amplificados confirmaram que os trematódeos classificados como Eurytrema coelomaticum eram filogeneticamente distintos daqueles identificados como E. pancreaticum. Os resultados deste estudo representam a primeira caracterização molecular de E. coelomaticum nas Américas e disponibiliza um método eficiente para diferenciar trematódeos digenéticos em animais domésticos.Palavras-chave: Trematoda bovino, Eurytrema spp., PCR, filogenia.

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Section: Introductionmentioning

confidence: 99%

Molecular characterization of Eurytrema coelomaticum in cattle from Paraná, Brazil

Figueira

Oliveira

Taroda

et al. 2014

Rev. Bras. Parasitol. Vet.

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“…Travassos (1944) reported the dimensions of E. coelomaticum eggs (50 -42 μm length and 23 -30 μm width). Tang (1950) did the same for E. pancreaticum eggs (55 -41 μm length and 38 -28 μm width). Travassos (1944) also stated that mature eggs are dark brown and thick shelled, while immature ones are lighter in color.…”

Section: Introductionmentioning

confidence: 81%

Additional study of the morphology of eggs and miracidia of Eurytrema coelomaticum (Trematoda)

et al. 2015

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SummaryThe morphology of the eggs and miracidia of Eurytrema coelomaticum was analyzed. The eggshell presented different composition according to the maturation stage of the egg, as seen by distinct birefringence after polarized light microscopic analysis. Detailed morphology of the brownish eggs, measuring 44.97±3.83 (38.67 -51.40) μm in length and 30. 71±2.54 (25.79 -34.47) μm in width, and the different electrondensities of the inner, middle and outer layers of the eggs were determined using light (bright fi eld and DIC) and electronic microscopy (scanning and transmission). The morphometry of the eggs is in accordance to those measures presented in previous studies. The miracidia were observed using light microscopy, inside and outside the egg. It was possible to observe the terebratorium at the anterior end of the body, the ciliated epidermal plates, placed according the formula E=2E 1 + 2E 2 , and the interepidermal ridge, but eyespots were not observed. The excretory vesicle was laterally placed in the posterior middle of the body and the germ balls were observed. The miracidium inside the egg was observed by routine techniques, but the miracidium was not well preserved. Thus, the eggs were processed using high pressure-freeze substitution technique and reasonable preservation was achieved. The cilia covering the larval body, with a typical ciliary organization, the terebratorium with folds and the junction of the epidermal ciliated cell with the larval body were seen. For the fi rst time detailed information on the eggs and miracidia of E. coelomaticum is given, which can be the basis for new studies.

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“…No presente estudo, os esporocistos de segunda geração de E. coelomaticum, foram observados migrando pelos espaços hemolinfáticos, próximos à porção final do intestino dos moluscos e emergiram utili zando a abertura respiratória do molusco. Outros autores também verificaram que os esporocistos de segunda geração deixavam o molusco, através do pneumós-toma, entretanto, nenhum deles documentou, fotograficamente o fato, como foi fei to no presente estudo (TANG 1950;SAKAMOTO et aI. 1980).…”

Section: Discussionunclassified

“…O acompanhamento da rota de migração de E. coelomaticum pela massa visceral de B. sil17ilaris foi realizado através de dissecações periódicas dos moluscos, de acordo com o tempo necessário para o desenvolvimento de cada estágio larval de E. coelomalicum e E. pancreaticum no primeiro hospedeiro intermediário, disponível na literatura (TANG 1950;TANG & T ANG 1977;SAKAMOTO et aI. 1980;MATTOS 1987;KOZUTS UMl & ITAGAKI 1989).…”

Section: Methodsunclassified

“…Caixa postal 700, 90001-970 Po rto Alegre, Rio Gran de do Sul, Bras il. E-mail: s bamato@vortex.ufrgs.br TANG (1950), ao dissecar espécimes de B. sim ilaris infectados com E. pancreaticllm após 165 dias de infecção, observou alguns esporocistos de segunda geração totalmente desenvolvidos, migrando para a porção anterior da abertura respiratória do molusco. TANG & TANG (1977) observaram que o desenvolvimento de E. coelomaticum em B. similaris durava seis meses e, que após este período os esporocistos de segunda geração migravam para o pneumóstoma.…”

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Desenvolvimento de Eurytrema coelomaticum (Giard & Billet) (Digenea, Dicrocoeliidae) em Bradybaena similaris (Férussac) (Gastropoda, Xanthonychidae)

Brandolini

Amato

2001

Rev. Bras. Zool.

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ABSTRACT. Development of ElIrylrellla coel omaliclIlII (Giard & Billet) (Digenea, Dicrococliidac) in Brat/ybaella silllilaris (Férussac) (Gastropoda, Xanthonychidae). To follow the larval development of Ewytrema coelomaliclIm (Giard & Billel, 1892) in 8radybaena similaris (Férussac, 1821) snails were separaled in three classes llsing lhe shell diameter: Class A (14.5-10 .2 mm), Class B (10.1-6.9 mm) and Class C (6.8-2.6 mm). Only snails belonging to classes A and B acquired the inteclion.Specimens of E. coelomalicum removed from lhe pancrealic ducts were exposed to lhree physiological solutions: Earle, Locke and saline 0.85%, to obtain eggs for the experimental infections. The Locke solution induced the best egg release. The route ofmigration lhe intramolluscan development oI' E. coelomalicum was stlldied with the aid 01" histology. The minimal period of intramolluscan development, ending aI lhe expelling of daughler sporocysts, was 107 days for the snails intecled in March, and 79 days for lhe snails infecled in November. The Sludenl "t" test and the Chi-square lest showed a significant difterence (u = 5%) between the two periods, although the mean lemperalure regislered during the experiments did not signiticanlly diftered (u = 5%). The eliminalion of daughter sporocysls occurred lhrough lhe snail's pneumostome, and always aI night. Most sporocysts were eliminated aI intervals thal varied between one to three days, without reglllarity. The time ofelimination ofthe dallghter sporocysts was ditlerenl for the two intection period studied: 12 weeks for the snails infecled in March, and llu'ee weeks for lhos e infecled in November. Posilive correlalion between the number of sporocysts expelled by the snail hOSI and higher lemperatures registered in lhe laboralOlY was observed. This correlation was more evident in November infeclion. KEY WORDS.

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Studies on the Life History of Eurytrema pancreaticum Janson, 1889

Cited by 26 publications

References 3 publications

Molecular characterization of Eurytrema coelomaticum in cattle from Paraná, Brazil

Molecular characterization of Eurytrema coelomaticum in cattle from Paraná, Brazil

Additional study of the morphology of eggs and miracidia of Eurytrema coelomaticum (Trematoda)

Desenvolvimento de Eurytrema coelomaticum (Giard & Billet) (Digenea, Dicrocoeliidae) em Bradybaena similaris (Férussac) (Gastropoda, Xanthonychidae)

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