Studies on the Mechanism of Immunity in the Rat to the Nematode, Nippostrongylus Muris12

Thorson, Ralph E.

doi:10.1093/oxfordjournals.aje.a119584

Cited by 29 publications

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“…Some studies reported that parasites secret various enzymes, including lipases, 26 a fibrionlytic enzyme, 27 and superoxide dismutase, 28 in the host. Furthermore, protease secretion has been detected in rats and humans infected with Strongyloides sp.…”

Section: Discussionmentioning

confidence: 99%

Protease Secreted by the Infective Larvae of Angiostrongylus Cantonensis and Its Role in the Penetration of Mouse Intestine

Lee

Yen

2005

The American Journal of Tropical Medicine and Hygiene

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The infective third-stage larvae of Angiostrongylus cantonensis secrete a proteolytic enzyme that is thought to be essential for both larval penetration into the intestinal wall of the host and full development. Protease activity in these larvae during culture in vitro was determined by zymography, pH optimum, and substrate and inhibitor specificity. Excretory-secretory (ES) products of the third-stage larvae showed protease activity as three bands with molecular masses of 66, 30, and 23 kD by gelatin zymography. The optimal pH value for this protease activity was 10.0. The protease was found to have collagenolytic as well as elastinolytic activity, but these activities were inhibited by serine protease or metalloprotease inhibitors. The importance of this protease in larval penetration of the intestinal wall and entering the blood stream was observed in vitro by cocultured third-stage larvae of A. cantonensis with specific protease inhibitors in the intestines of BALB/c mice. The penetration rates of larvae significantly decreased when serine protease or metalloprotease inhibitors were added to the intestines. These results showed that serine protease and metalloprotease in ES products of A. cantonensis third-stage larvae are associated with larval penetration of the intestinal walls of mice.

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Section: Discussionmentioning

confidence: 99%

Protease Secreted by the Infective Larvae of Angiostrongylus Cantonensis and Its Role in the Penetration of Mouse Intestine

Lee

Yen

2005

The American Journal of Tropical Medicine and Hygiene

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“…Thorson (1956) demonstrated a lipolytic enzyme from the eosophageal glands of hookworms whose action could be inhibited by the serum of an immune dog. Thorson (1953) also demonstrated that infective larvae of N . murk possessed a lipase.…”

Section: Excretory and Secretory Productsmentioning

confidence: 91%

The Nature and Origin of the Functional Antigens in Helminth Infections

Soulsby

1963

Annals of the New York Academy of Sciences

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“…These papers followed a logical process of investigation starting with studies of the mechanism of immunity to Nippostrongylus brasiliensis (muris) in the rat. 2,3 Eye catching, from a vaccination point of view, were follow-up studies in the dog hookworm Ancylostoma caninum. Firstly, a study which demonstrated proteolytic activity in extracts of the oesophagus from the adult parasite that could be inhibited by immune serum 4 and another that showed the induction of acquired immunity in dogs by the injection of similar extracts.…”

Section: Introductionmentioning

confidence: 99%

Proteases in Blood-Feeding Nematodes and Their Potential as Vaccine Candidates

Knox

2011

Advances in Experimental Medicine and Biology

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Parasitic nematodes express and secrete a variety of proteases which they use for many purposes including the penetration of host tissues, digestion of host protein for nutrients, evasion of host immune responses and for internal processes such as tissue catabolism and apoptosis. For these broad reasons they have been examined as possible parasite control targets. Blood-feeding nematodes such as the barber-pole worm Haemonchus contortus that infect sheep and goats and the hookworms, Ancylostoma spp. and Necator americanus, affecting man, use an array of endo- and exopeptidases to digest the blood meal. Haemoglobin digestion occurs by an ordered and partly conserved proteolytic cascade. These proteases are accessible to host immune responses which can block enzyme function and lead to parasite expulsion and/or death. Thus they are receiving attention as components of vaccines against several parasitic nematodes of social and economic importance.

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Studies on the Mechanism of Immunity in the Rat to the Nematode, Nippostrongylus Muris12

Cited by 29 publications

References 0 publications

Protease Secreted by the Infective Larvae of Angiostrongylus Cantonensis and Its Role in the Penetration of Mouse Intestine

Protease Secreted by the Infective Larvae of Angiostrongylus Cantonensis and Its Role in the Penetration of Mouse Intestine

The Nature and Origin of the Functional Antigens in Helminth Infections

Proteases in Blood-Feeding Nematodes and Their Potential as Vaccine Candidates

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