Studies on the mechanism of the stimulation of glycolysis and respiration by K+ in Saccharomyces cerevisiae

Peña, Antonio; Cinco, G.; Puyou, Armando Gómez; Tuena, M.

doi:10.1016/0005-2728(69)90187-x

Cited by 39 publications

(20 citation statements)

References 5 publications

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“…The transport of K + can occur with both glucose and ethanol but, with the latter, as expected, it requires the presence of oxygen. Moreover, as occurs with S. cerevisiae (Peña et al ., ), KCl produced a clear increase of respiration; besides, as previously reported for S. cerevisiae (Peña et al ., ), respiration was particularly increased by varying the pH from 4.0 or 6.0 to 7.5. As with S. cerevisiae , the effect of KCl was proportionally higher at pH 4.0 and 6.0 than at 7.5.…”

Section: Discussionsupporting

confidence: 87%

“…At the higher pH values, even in the absence of a substrate, a small acidification was observed (not shown); besides, with ethanol as substrate: (a) as described previously, acidification could only be seen when H 2 O 2 was included; (b) this acidification was inhibited by 10 μ m CCCP (not shown); and (c) the rate of acidification was significantly smaller than with glucose. As described long ago for S. cerevisiae (Peña et al ., ), acidification was clearly increased at all pH values when K + was added. With glucose as substrate: (a) it was larger and faster than with ethanol; (b) it was not inhibited by 20 μ m CCCP (not shown); (c) however, it was inhibited by 200 μ m cyanide (not shown).…”

Section: Resultsmentioning

confidence: 84%

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Characterization of glycolytic metabolism and ion transport of Candida albicans

Calahorra

Sánchez

Peña

2012

Yeast

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The main energetic pathways, fermentation and respiration, and the general ion transport properties of Candida albicans were studied. Compared to Saccharomyces cerevisiae, we found that in C. albicans: (a) the cell mass yield when grown in YPD was significantly larger; (b) it required longer times to be starved of endogenous substrates; (c) ethanol production was lower but significant; (d) respiration was also lower; (e) it showed a small activity of an alternative oxidase; (f) fermentation and oxidative phosphorylation seemed to compete for both ADP and NADH; and (g) NADH levels were lower. Regarding ion transport and compared to S. cerevisiae: (a) the general mechanism was similar, with a plasma membrane H + -ATPase that generates both a plasma membrane ΔpH and a ΔΨ, the latter being responsible for driving K + inside; (b) its acidification capacity is slightly smaller and less sensitive to activation by high pH; and (c) the presence of K + results in a large activation of both respiration and fermentation, most probably due to the energy required in the process. ADP produced by H + -ATPase stimulation by high pH or the addition of K + at low pH results in the increase of both respiration and fermentation.

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Section: Discussionsupporting

confidence: 87%

Section: Resultsmentioning

confidence: 84%

Characterization of glycolytic metabolism and ion transport of Candida albicans

Calahorra

Sánchez

Peña

2012

Yeast

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“…We introduced the fluorescent dye BCECF-AM, which measures vacuolar pH in yeast (11,30), or yeast pHluorin, a green fluorescent protein analog that measures cytosolic pH (31), into wild-type and vma mutant cells and studied the dynamic adjustments of cytosolic and vacuolar pH in response to glucose and KCl addition. As described above, both glucose and potassium ion are strongly implicated in cytosolic and vacuolar pH homeostasis (25,26,32,48). Fig.…”

Section: Resultsmentioning

confidence: 87%

Vacuolar and Plasma Membrane Proton Pumps Collaborate to Achieve Cytosolic pH Homeostasis in Yeast

Martínez-Muñoz¹,

Kane

2008

Journal of Biological Chemistry

240

238

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Vacuolar proton-translocating ATPases (V-ATPases) play a central role in organelle acidification in all eukaryotic cells. To address the role of the yeast V-ATPase in vacuolar and cytosolic pH homeostasis, ratiometric pH-sensitive fluorophores specific for the vacuole or cytosol were introduced into wild-type cells and vma mutants, which lack V-ATPase subunits. Transiently glucose-deprived wild-type cells respond to glucose addition with vacuolar acidification and cytosolic alkalinization, and subsequent addition of K ؉ ion increases the pH of both the vacuole and cytosol. In contrast, glucose addition results in an increase in vacuolar pH in both vma mutants and wild-type cells treated with the V-ATPase inhibitor concanamycin A. Cytosolic pH homeostasis is also significantly perturbed in the vma mutants. Even at extracellular pH 5, conditions optimal for their growth, cytosolic pH was much lower, and response to glucose was smaller in the mutants. In plasma membrane fractions from the vma mutants, activity of the plasma membrane proton pump, Pma1p, was 65-75% lower than in fractions from wildtype cells. Immunofluorescence microscopy confirmed decreased levels of plasma membrane Pma1p and increased Pma1p at the vacuole and other compartments in the mutants. Pma1p was not mislocalized in concanamycin-treated cells, but a significant reduction in cytosolic pH under all conditions was still observed. We propose that short-term, V-ATPase activity is essential for both vacuolar acidification in response to glucose metabolism and for efficient cytosolic pH homeostasis, and long-term, V-ATPases are important for stable localization of Pma1p at the plasma membrane.The importance of V-ATPases 3 for acidification of the vacuole/lysosomes, Golgi apparatus, and endosomes of eukaryotic cells is well established (1, 2). Multiple cellular processes, including secondary transport of ions and metabolites, maturation of iron transporters, endocytic and biosynthetic protein sorting, and zymogen activation depend on compartment acidification and have been linked to V-ATPase activity (1, 3). In some cells such as macrophages, V-ATPases play specialized roles that clearly include regulation of cytosolic pH (4, 5). However, although V-ATPases pump protons from the cytosol into organelles in all cells, they are not generally believed to play a major role in cytosolic pH regulation.The yeast Saccharomyces cerevisiae has emerged as a major model system for eukaryotic V-ATPases. One reason for this is that yeast mutants lacking all V-ATPase activity (vma mutants) are viable, but loss of V-ATPase activity in eukaryotes other than fungi is lethal (6 -9). Yeast vma mutants do exhibit a set of distinctive phenotypes, however, that includes the inability to grow at pH values lower than 3 or higher than 7 and sensitivity to high extracellular calcium concentrations (2). This Vma Ϫ phenotype suggests a perturbation of pH homeostasis in these cells that is not fully understood. It has been suggested that vma mutants survive at low extracellular p...

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“…Potassium ion is a fundamental cation for Saccharomyces cerevisiae ; it stimulates fermentation (Rothstein and Demis, 1953) and respiration (Peña et al , 1969; Peña, 1975). It has also been reported that K + is partly accumulated with bicarbonate as a counter‐ion (López et al , 1999) and it participates in the modulation of the internal pH in yeast (Calahorra et al , 1998; Ramírez et al , 1996).…”

Section: Introductionmentioning

confidence: 99%

In situ study of K⁺ transport into the vacuole of Saccharomyces cerevisiae

Martínez-Muñoz

Peña

2005

Yeast

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Permeable spheroplasts were prepared from two strains of Saccharomyces cerevisiae by incubating with zymolyase without a permeabilizing agent. The loss of the plasma membrane barrier was confirmed by the nucleotide release, the activity of glucose 6-phosphate dehydrogenase with external substrates and by the effects on respiration of mitochondrial substrates and ADP. Mitochondrial integrity was maintained, as shown by respiration with lactate, pyruvate, glucose and ethanol, and its acceleration by ADP showed a coupled respiration. Potassium uptake into the vacuole was measured with a selective electrode and found to be taken up effectively by spheroplasts only in the presence of Mg-ATP; it was reverted by CCCP and PCP and inhibited by bafilomycin A 1 , but not by sodium vanadate or sodium azide. Potassium ions did not alter of the vacuole, followed with oxonol V, but caused vacuolar alkalinization, as followed with pyranine. The increase of vacuolar pH was non-selective and observed at 50-200 mM of several monovalent cations. Isolated vacuoles with pyranine inside showed similar changes of the internal pH in the presence of KCl. Results indicate that some strains do not require a permeabilizing agent to directly access the vacuole in spheroplasts prepared with zymolyase. The hypothesis about the existence of a K + /H + antiporter in the vacuolar membrane of S. cerevisiae is discussed.

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Studies on the mechanism of the stimulation of glycolysis and respiration by K+ in Saccharomyces cerevisiae

Cited by 39 publications

References 5 publications

Characterization of glycolytic metabolism and ion transport of Candida albicans

Characterization of glycolytic metabolism and ion transport of Candida albicans

Vacuolar and Plasma Membrane Proton Pumps Collaborate to Achieve Cytosolic pH Homeostasis in Yeast

In situ study of K⁺ transport into the vacuole of Saccharomyces cerevisiae

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Studies on the mechanism of the stimulation of glycolysis and respiration by K+ in Saccharomyces cerevisiae

Cited by 39 publications

References 5 publications

Characterization of glycolytic metabolism and ion transport of Candida albicans

Characterization of glycolytic metabolism and ion transport of Candida albicans

Vacuolar and Plasma Membrane Proton Pumps Collaborate to Achieve Cytosolic pH Homeostasis in Yeast

In situ study of K+ transport into the vacuole of Saccharomyces cerevisiae

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Product

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In situ study of K⁺ transport into the vacuole of Saccharomyces cerevisiae