Studies on the mechanism of PMN activation I. by dextran sulfates

Müller, J.; Botzenhardt, U.; Alföldy, P; Lemmel, E.-M.

doi:10.1007/bf00319921

Cited by 7 publications

(2 citation statements)

References 11 publications

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“…Hier sei nur auf die geradezu klassische Pneumokokkensepsis nach Splenektomie beim Kinde hingewiesen [51]. Immunmodulatoren miissen nicht nur ihre Niitzlichkeit, sondern auch ihre Unsch/idlichkeit erst noch unter Beweis stellen [13,18,31,34,42,44,49,50]. die ausschlieBlich explorative Laparotomie, verursacht eine 3 Wochen und 1/inger nachweisbare St6rung der T-Lymphocyten bzw.…”

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41. Immunreaktivit�t, ein pr�operativ fa�barer Parameter?

Kremer

Früh

Homann

et al. 1983

Langenbecks Arch Chiv

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Immunoreactivity, a Preoperative Assessable Risk Parameter?Summary. The perioperative risk of 44 patients with a malignoma of the esophagus, stomach or colon was estimated by a check list of risk parameters. There was no satisfactory correlation between preoperative risk estimation and postoperative complications. A remarkable increase of this correlation was achieved by additional measurement of the phagocytosis activity of the PNM tested by the Zymosan induced chemiluminescence. The predicting value of this retrospective evaluated parameter of operative risk estimation and immunoreactivity was further investigated by a prospective study on 132 patients with an intestinal carcinoma. Zusammeufassung. Bei 44 Patienten mit einem Malignom des Oesophagus, Magens oderColons wurde das operative Risiko pr~ioperativ an Hand einer Risikocheckliste objektiviert. Eine befriedigende Klassifizierung in 3 Risikogruppen in Korrelation zur postoperativen Komplikationsrate war nur durch zus/itzliche Berficksichtigung der Phagocytosef'~ihigkeit der PNM gemessen fiber die Zymosan-induzierte Chemiluminescenz im Vollblut der Patienten m6glich. Die Risikowertigkeit dieses retrospektiv erhobenen Risikoparameters der Immun-reaktivit~it wurde prospektiv an 132 Malignompatienten untersucht. Auch in der prospektiven Studie lieB sich eine deutlich bessere Trennsch~irfe der pr/ioperativen Risikobeurteilung erzielen. Sehliisselwiirter: Risikobeurteilung -Immunreaktivit/it -Phagocytose -Chemiluminescenz.

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41. Immunreaktivit�t, ein pr�operativ fa�barer Parameter?

Kremer

Früh

Homann

et al. 1983

Langenbecks Arch Chiv

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“…Combining the syn thetic adjuvants dimethyldioctadecylammonium bro mide (DDA) and dextran sulfate (DXS) resulted in a synergistically enhanced humoral response to sheep red blood cells (SRBC) [14], The synergy is considered to be the consequence of different but complementary mechanisms responsible for the immunostimulating activity of either component. The surfactant DDA and the polyanion DXS differ in physicochemical [14], biological [2,3,7,9,16,17,[20][21][22][24][25][26], and immunostimulating [12 and accompanying paper] properties, and therefore we expected distinct modes of action. Consequently, synergistic stimulatory ac tivities of adjuvants might be expected if the modes of action are distinct.…”

Section: Introductionmentioning

confidence: 99%

Synergistic Effects of Synthetic Adjuvants on the Humoral Immune Response

Hilgers¹,

Snippe²,

Jansze³

et al. 1986

Int Arch Allergy Immunol

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The effect of combinations of adjuvants on the humoral immune response to sheep red blood cells (SRBC) as antigen was investigated. Adjuvants belonging to two categories differing in physicochemical properties were used: surfactants (N, N-dioctadecyl-N′, N′-bis-(2-hydroyethyl)propanediamine (CP-20,961), dimethyldioctadecylammonium bromide (DDA), neutrally charged liposomes, polyol (L 101 and L 121) and polyanions [dextran sulfate (DXS), liquoid and suramine]. All adjuvants but suramine augmented humoral responses to 2 × 10⁷ SRBC measured by the number of direct anti-SRBC plaque-forming cells (PFC) in the spleen. The response to 2 × 10⁶ SRBC was enhanced considerably by L 121 and DXS but hardly or not at all by the other adjuvants. Combinations of two adjuvants were made at distinct ratios (1:3, 2:2, and 3:1) and injected intraperitoneally with 2 × 10⁶ SRBC. Low responses (5 × 10³ PFC per spleen) were induced by combinations of liquoid or suramine with DDA or DXS, and by combinations of CP-20,961 liposomes, L 101 or L 121 with DDA. Combinations of the surfactants DDA, CP-20,961, liposomes, L 101 or L 121 with DXS evoked responses which were significantly higher than the sum of responses supported by the single adjuvants. Ratios of 1:3 or 2:2 (surfactant: DXS) resulted in the most effective combinations. The data obtained suggest that only adjuvants derived from two different physicochemical groups are able to act synergistically.

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Evidence of Polymorphonuclear Neutrophils (PMN) Activation in Patients With Insulin-Dependent Diabetes Mellitus

Wierusz‐Wysocka¹,

Wysocki²,

Siekierka³

et al. 1987

Journal of Leukocyte Biology

111

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Polymorphonuclear neutrophils' chemotaxis, surface charge, superoxide anions generation, NBT (nitro blue tetrazolium) reduction and intracellular lysozyme, and beta-glucuronidase content were estimated in patients with type I diabetes mellitus in a similar state of metabolic control. The chemotaxis of diabetic cells toward bacterial chemotactic factors was similar to controls, whereas migration toward complement-derived chemoattractants was significantly reduced. Polymorphonuclear neutrophils isolated from diabetic patients, when unstimulated, produced significantly greater amounts of superoxide anions and reduced NBT more efficiently. They also revealed reduced surface charge and lower intracellular content of lysozyme, whereas beta-glucuronidase content was similar to controls. The results obtained seem to indicate that neutrophils in patients with insulin-dependent diabetes manifest signs of being in the activated state. The possible mechanisms of such stimulation are discussed.

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Studies on the mechanism of PMN activation I. by dextran sulfates

Cited by 7 publications

References 11 publications

41. Immunreaktivit�t, ein pr�operativ fa�barer Parameter?

41. Immunreaktivit�t, ein pr�operativ fa�barer Parameter?

Synergistic Effects of Synthetic Adjuvants on the Humoral Immune Response

Evidence of Polymorphonuclear Neutrophils (PMN) Activation in Patients With Insulin-Dependent Diabetes Mellitus

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