Studies on the mechanism of energy metabolism via <scp>AMPK</scp>/<scp>PGC</scp>‐1α signaling pathway induced by compatibility of <i>Ligusticum chuanxiong</i> Hort and <i>Gastrodia</i>

Ni, Ni; Yang, Luping; Lin, Xiao; Yang, Hong; Shen, Lan

doi:10.1002/ptr.7623

Cited by 5 publications

(2 citation statements)

References 53 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Subsequently, it forms a transcription initiation complex in conjunction Transcription-Factor Binding Motif (TFBM) and mitochondrial RNA polymerase, thus regulating mitochondrial biogenesis and function [21] . Research has substantiated that the excessive activation of the AMPK/PGC-1α signaling pathway significantly contributes to the regulation of proliferation, survival and invasiveness of many cancer cells [22] . The findings indicate a significant decrease in the relative expression of MPK and PGC-1α mRNA and protein in the colorectal cancer group compared to the blank control group.…”

Section: Table 2: Effects Of Resveratrol On Invasion and Apoptosis Of...mentioning

confidence: 99%

The Mechanism of Resveratrol on Colorectal Cancer by Regulating AMPK/PGC-1-Alpha Signaling Pathway

Zhang,

Shi

et al. 2023

IJPS

View full text Add to dashboard Cite

The objective of this study is to examine the impact of resveratrol on the activation of the AMP-activated protein kinase/peroxisome proliferator gamma receptor coactivator 1-alpha mechanism of signal pathway on colorectal cancer. Set up blank control group, colorectal cancer group and resveratrol group. The proliferation ability of three groups of cells was assessed using cell counting kit 8, protein expression was detected using Western blot, the expression level of related messenger ribonucleic acid was determined using quantitative polymerase chain reaction, and the migration and invasion ability of cells was assessed using the Transwell method. The resveratrol group exhibited a significantly elevated expression level of apoptotic proteins Fas, Bcl-2-associated X protein, and Fas ligand compared to the colorectal cancer group, and the expression level of B-cell lymphoma 2 was found to be significantly reduced compared to the colorectal cancer group. In the colorectal cancer group alpha, the relative expression of messenger ribonucleic acid and protein for mitogen-activated protein kinase and peroxisome proliferator-activated receptor-gamma coactivator was found to be significantly lower compared to the blank control group; the relative expression of messenger ribonucleic acid and protein in the resveratrol group cells, specifically mitogen-activated protein kinase and peroxisome proliferator gamma receptor coactivator 1-alpha, was found to be significantly higher compared to the colorectal cancer group. Resveratrol exhibits the potential to diminish the viability of colorectal cancer cells, impede the growth and metastasis of such cells, as well as prompt apoptosis in colorectal cancer cells. The mechanism potentially exhibits a correlation between the regulations of AMP-activated protein kinase/ peroxisome proliferator gamma receptor coactivator 1-alpha signaling pathway.

show abstract

Section: Table 2: Effects Of Resveratrol On Invasion and Apoptosis Of...mentioning

confidence: 99%

The Mechanism of Resveratrol on Colorectal Cancer by Regulating AMPK/PGC-1-Alpha Signaling Pathway

Zhang,

Shi

et al. 2023

IJPS

View full text Add to dashboard Cite

show abstract

“…PGC-1α is a powerful transcriptional regulator and was originally identified as a transcriptional coactivator of mitochondrial function ( 21 , 22 ) and brown fat thermogenesis ( 23 ). It can effectively regulate oxidation and antioxidant processes ( 24 , 25 ) and is involved in the regulation of mitochondrial function ( 26 ) and the development of a variety of diseases ( 27 ).…”

Section: Introductionmentioning

confidence: 99%

ZLN005 improves the protective effect of mitochondrial function on alveolar epithelial cell aging by upregulating PGC-1α

Ma,

Gao,

Qiu

et al. 2023

J Thorac Dis

View full text Add to dashboard Cite

Background Idiopathic pulmonary fibrosis (IPF) is a chronic and fatal pulmonary interstitial disease that usually occurs in the elderly. The senescence of alveolar epithelial cells (AECs) is an important mechanism of IPF. The AECs of patients with IPF have lower expression of peroxisome proliferator-activated receptor-γ coactivator-1 alpha (PGC-1α), which has been shown to play an important role in maintaining mitochondrial morphology and energy metabolism. This study sought to explore the mechanism by which ZLN005 improves mitochondrial function by upregulating PGC-1α to protect AECs from aging. Methods Western blot was used to detect the expression of PGC-1α, mitochondrial synthesis protein nuclear respiratory factor-1 (NRF-1), and p21 WAF1 in the lung tissue of the IPF patients and the mice with bleomycin (BLM)-induced pulmonary fibrosis. A549 cells and mice AEC2 cells were treated with hydrogen peroxide (H 2 O 2 ) to construct cell senescence models. Cell senescence was detected by senescence-associated beta-galactosidase staining. The mitochondrial respiratory function was measured, including the adenosine triphosphate (ATP) generation, reactive oxygen species (ROS) level, changes in cell membrane potential, and energy metabolism. Using lentivirus as a vector and using gene editing technology to over express (upPGC-1α) and knockdown PGC-1α (shPGC-1α) in the A549 cells. The PGC-1α agonist ZLN005 was used to pretreat the A549 and shPGC-1α A549 cells, and cell aging and mitochondrial respiratory function were observed. Results The Western blot and immunofluorescence assays showed that the expression of PGC-1α and NRF-1 was decreased in the lung tissues of the IPF patients and BLM-induced mice pulmonary fibrosis model, while the expression of p21 WAF1 was increased. The results of the immunofluorescence and mitochondrial function experiments also indicated that the expression of PGC-1α and mitochondrial synthesis protein NRF-1 were decreased in the senescent cells. Further, the mitochondrial morphology was abnormal and the mitochondrial function was impaired. PGC-1α was involved in the AEC senescence by regulating mitochondrial morphology and function. Treatment with the agonist of PGC-1α (i.e., ZLN005) blocked the H 2 O 2 -induced cell senescence by enhancing the expression of PGC-1α. Conclusions These results provide preliminary insights into the potential clinical application of ZLN005 as a novel therapeutic agent for the treatment of IPF.

show abstract

Cadmium induced time-dependent kidney injury in common carp via mitochondrial pathway: Impaired mitochondrial energy metabolism and mitochondrion-dependent apoptosis

et al. 2023

View full text Add to dashboard Cite

Studies on the mechanism of energy metabolism via AMPK/PGC‐1α signaling pathway induced by compatibility of Ligusticum chuanxiong Hort and Gastrodia

Cited by 5 publications

References 53 publications

The Mechanism of Resveratrol on Colorectal Cancer by Regulating AMPK/PGC-1-Alpha Signaling Pathway

The Mechanism of Resveratrol on Colorectal Cancer by Regulating AMPK/PGC-1-Alpha Signaling Pathway

ZLN005 improves the protective effect of mitochondrial function on alveolar epithelial cell aging by upregulating PGC-1α

Cadmium induced time-dependent kidney injury in common carp via mitochondrial pathway: Impaired mitochondrial energy metabolism and mitochondrion-dependent apoptosis

Contact Info

Product

Resources

About