Studies on the Mechanism of Action of Staphylocoagulase

Rubinstein, Herbert M.

doi:10.1111/j.1365-2141.1958.tb06035.x

Cited by 6 publications

(1 citation statement)

References 40 publications

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“…Complexing to S. aureus was assessed by mixing equal volumes of plasma samples with the supernatant of a saline suspension of isolated S. aureus at a cell density of approximately 1 X 10" bacteria/ml [35]. Again, the unclotted material was analyzed by means of two-dimensional immunoelectrophoresis.…”

Section: Complexing Of Prothrombin To Staphylococcus Aureus Coagulasementioning

confidence: 99%

Prothrombin “Mexico city,” an asymptomatic autosomal dominant prothrombin variant

Valls-de-Ruiz

Ruı́z-Argüelles

et al. 1987

American J Hematol

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A functionally normal but structurally abnormal prothrombin variant was found in a Mexican family. Immunoisoelectricfocusing studies revealed that this variant has a more acidic isoelectric point (4.01) than normal prothrombin (4.29), but it proved to have a normal molecular weight as assessed by sodium-dodecyl-sulphate polyacrylamide gel electrophoresis. Two-dimensional immunoelectrophoresis studies showed an abnormal cleavage of the prothrombin molecule by factor Xa and Echis carinatus venom as well, despite the fact that both yield functionally normal thrombin molecules. Finally, the ability of the molecule to bind calcium ions as well as its overall antigenic structure were investigated and found to be normal. These results taken together suggest a simple (substitution or translocation) mutation at the fragment 2 level. Since this prothrombin variant is different from others described previously, the name "Mexico City" is proposed to identify it.

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Section: Complexing Of Prothrombin To Staphylococcus Aureus Coagulasementioning

confidence: 99%

Prothrombin “Mexico city,” an asymptomatic autosomal dominant prothrombin variant

Valls-de-Ruiz

Ruı́z-Argüelles

et al. 1987

American J Hematol

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An histo-morphological evaluation of ninety surgically excised human umbilical vein grafts

Gill

Guzman

Guidoin

et al. 1989

J. Biomed. Mater. Res.

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Morphological, histological, and scanning electron microscopy examinations were performed on 90 surgically excised human umbilical vein grafts. Most of the explanted grafts were removed because of thrombosis or infection and were removed typically from a patient in the mid 60s and after an average duration of implantation of 11 months. Multiple structural defects were found including deep folds, breaks on the luminal surface, and delamination. These areas as well as anastomotic sites represented potential areas for thrombotic accumulation. A higher incidence of infection was observed in grafts composed of 2 or 3 segments. Bacteria were often found in folds and could be seen invading the wall of the prosthesis. In addition, bacteremic colonization was often seen in noninfected grafts. The late aneurysmal formations were also of particular concern. The biodegradation of the wall and the disruption of the polyester mesh were the probable causes. The second generation Dardik Biograft aimed at reducing these formations. The success of this new processing remains to be evaluated.

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