Studies on the methanolysis of small amounts of purified phospholipids for gas chromatographic analysis of fatty acid methyl esters

Eder, Klaus; Reichlmayr‐Lais, Anna M.; Kirchgeßner, M.

doi:10.1016/0021-9673(92)87054-c

Cited by 29 publications

(9 citation statements)

References 47 publications

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“…Overall, cardiolipin (CL) and phosphatidylethanolamine (PE) showed good to adequate conversions to FAMEs (69-91%) while the conversions of the triacylglycerol (TAG), phosphatidylglycerol (PG) and phosphatidylcholine (PC) standards were poor to adequate (38-71%). Not surprisingly, conversions of sphingomyelin (SM, an amide lipid) were poor (25-36%); conversions of sphingolipids are known to require vigorous acidic conditions [10,35]. SM was introduced since 3-hydroxy fatty acids in bacterial lipids are found in lipopolysaccharides attached via amide bonds.…”

Section: Comparison Of Methods a B And Cmentioning

confidence: 99%

Comparison of three GC/MS methodologies for the analysis of fatty acids in Sinorhizobium meliloti: Development of a micro-scale, one-vial method

Basconcillo

McCarry

2008

Journal of Chromatography B

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Section: Comparison Of Methods a B And Cmentioning

confidence: 99%

Comparison of three GC/MS methodologies for the analysis of fatty acids in Sinorhizobium meliloti: Development of a micro-scale, one-vial method

Basconcillo

McCarry

2008

Journal of Chromatography B

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“…The conjugate was dissolved in ethanol and the absorption spectra was measured from 200 to 600 nm. Next, the new compound was hydrolyzed and methylated in 3 M methanolic HCl [31]. The product of the reaction was extracted with hexane/water (2:1, v/v) and the organic phase was analyzed with a Shimatzu 17A gas chromatograph with a 0.25 mm × 30 m Stabilwax capillary column (Resteck, Belfont, PA).…”

Section: Methodsmentioning

confidence: 99%

Anticancer properties of propofol-docosahexaenoate and propofol-eicosapentaenoate on breast cancer cells

et al. 2005

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Introduction Epidemiological evidence strongly links fish oil, which is rich in docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA), with low incidences of several types of cancer. The inhibitory effects of omega-3 polyunsaturated fatty acids on cancer development and progression are supported by studies with cultured cells and animal models. Propofol (2,6-diisopropylphenol) is the most extensively used general anesthetic-sedative agent employed today and is nontoxic to humans at high levels (50 µg/ml). Clinically relevant concentrations of propofol (3 to 8 µg/ml; 20 to 50 µM) have also been reported to have anticancer activities. The present study describes the synthesis, purification, characterization and evaluation of two novel anticancer conjugates, propofol-docosahexaenoate (propofol-DHA) and propofol-eicosapentaenoate (propofol-EPA).

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“…Phospholipid species were quantified as lipid P after separation of plasma extracts (15) by thin-layer chromatography (16). Fatty acids of cholesteryl esters in extracts (15) of lipoprotein fractions separated by thin-layer chromatography (17) were converted to methyl esters (18) and quantified in a Packard model 5890A gas chromatograph. Plasma and lipoprotein fractions were examined by electron microscopy after negative staining (19).…”

Section: Methodsmentioning

confidence: 99%

Lamellar lipoproteins uniquely contribute to hyperlipidemia in mice doubly deficient in apolipoprotein E and hepatic lipase

Bergeron

Kotite²,

Vergés³

et al. 1998

Proc. Natl. Acad. Sci. U.S.A.

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Remnants of triglyceride-rich lipoproteins containing apolipoprotein (apo) B-48 accumulate in apo E-deficient mice, causing pronounced hypercholesterolemia. Mice doubly deficient in apo E and hepatic lipase have more pronounced hypercholesterolemia, even though remnants do not accumulate appreciably in mice deficient in hepatic lipase alone. Here we show that the doubly deficient mice manifest a unique lamellar hyperlipoproteinemia, characterized by vesicular particles 600 Å-1,300 Å in diameter. As seen by negative-staining electron microscopy, these lipoproteins also contain an electron-lucent region adjacent to the vesicle wall, similar to the core of typical lipoproteins. Correlative chemical analysis indicates that the vesicle wall is composed of a 1:1 molar mixture of cholesterol and phospholipids, whereas the electron-lucent region appears to be composed of cholesteryl esters (about 12% of the particle mass). Like the spherical lipoproteins of doubly deficient mice, the vesicular particles contain apo B-48, but they are particularly rich in apo A-IV. We propose that cholesteryl esters are removed from spherical lipoproteins of these mice by scavenger receptor B1, leaving behind polar lipid-rich particles that fuse to form vesicular lipoproteins. Hepatic lipase may prevent such vesicular lipoproteins from accumulating in apo E-deficient mice by hydrolyzing phosphatidyl choline as scavenger receptor B1 removes the cholesteryl esters and by gradual endocytosis of lipoproteins bound to hepatic lipase on the surface of hepatocytes.Chylomicrons transport dietary fat and cholesterol into the blood and are metabolized in two discrete steps (1). First, chylomicron triglycerides and phospholipids are partially removed in extrahepatic tissues by lipoprotein lipase to yield chylomicron remnants (CR); second, the remnants are adsorbed onto hepatic parenchymal cells and undergo receptormediated endocytosis. Binding of CR to hepatocytes is mediated chiefly by the low-density lipoprotein (LDL) receptor and hepatic lipase (HL) (1). The LDL receptor-related protein can also bind CR that have been enriched with apolipoprotein (apo) E. Endocytosis of CR, leading to lysosomal catabolism, is primarily mediated by the LDL receptor. The LDL receptor and LDL receptor-related protein bind CR by means of apo E. Thus, apo E-deficient mice (2, 3) and humans (4) manifest massive accumulation of CR. Although CR clearance is impaired in HL-deficient mice (5), and conversion of very low-density lipoprotein (VLDL) remnants to LDL and LDL concentrations are reduced in HL-deficient humans (6) and mice (5), accumulation of remnant lipoproteins is limited (5-7). Recently, Mezdour et al. (8) have reported that mice doubly deficient in apo E and HL have considerably higher levels of cholesterol in lipoproteins with remnant characteristics than mice deficient only in apo E. Here we show that the doubly deficient mice uniquely manifest a pronounced lamellar hyperlipoproteinemia, in which lipoprotein particles resembling liposomes accumula...

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Studies on the methanolysis of small amounts of purified phospholipids for gas chromatographic analysis of fatty acid methyl esters

Cited by 29 publications

References 47 publications

Comparison of three GC/MS methodologies for the analysis of fatty acids in Sinorhizobium meliloti: Development of a micro-scale, one-vial method

Comparison of three GC/MS methodologies for the analysis of fatty acids in Sinorhizobium meliloti: Development of a micro-scale, one-vial method

Anticancer properties of propofol-docosahexaenoate and propofol-eicosapentaenoate on breast cancer cells

Lamellar lipoproteins uniquely contribute to hyperlipidemia in mice doubly deficient in apolipoprotein E and hepatic lipase

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