Studies on the mobilization of a maize transposable family,Ac/Ds, in pepper using In Vivo transient assay system

Abstract

A maize transposable family, Ac/Ds, has been successfully utilized as a mutagenizing agent not only in monocot but also in dicot. In order to develop insertional mutagenesis system in pepper, the mobility of Ac/Ds has been examined. In this study, the excision of the elements was monitored via transient assay system with protoplasts. Two different systems were developed and compared; one-and two-elements systems. In a one-element system, Ac alone was introduced into cells. As a two-element system, Ac and Ds were cloned into a single vector and were expressed in protoplasts. Our data showed that both Ac and Ds elements were highly mobile in pepper cells. This is the first report suggesting that Ac/Ds mediated gene tagging system could be successfully operated in pepper.

Efficient transient expression and transformation of PEG-mediated gene uptake into mesophyll protoplasts of pepper (Capsicum annuum L.)

“…Recently, however, we isolated mesophyll protoplasts from pepper and introduced the maize transposable family, Ac/Ds, by PEG treatment to examine its mobility in pepper nuclei. The results showed that both Ac and Ds elements were highly mobile in pepper protoplasts (Kim et al 2004a). Therefore, we are interested in studying the transposition of the Ac/Ds element with a long term goal of developing a gene tagging system and a method for transposon mutagenesis in pepper.…”