Studies on the Occurrence of Peptococcus Indolicus and Corynebacterium Pyogenes in Apparently Healthy Cattle

Sørensen, Gunner Høi

doi:10.1186/bf03547939

Cited by 15 publications

(1 citation statement)

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“…4. To date, only a few papers on P. indolicus :oagulase have been published. Recently, S0rensen reng, espe-ported the isolation of P. indolicus from bovine g incuba-summer mastitis (22)(23)(24)(25). Usually these strains 00°C, its were isolated together with Corynebacterium Effect of heating at neutral, acidic, and alkaline pH on the activity of P. indolicus peptocoagulase activity.…”

Section: Resultsmentioning

confidence: 99%

Peptocoagulase: clotting factor produced by bovine strains of Peptococcus indolicus

Switalski¹,

Schwam²,

Smyth³

et al. 1978

J Clin Microbiol

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The production of a clotting factor (peptocoagulase) by bovine clinical isolates of Peptococcus indolicus and its nature were investigated. Extracellular peptocoagulase was demonstrated in culture filtrates of 93% and cell associated with washed cell suspensions of 100% of the 75 isolates tested. Both citrated and heparinized plasma were clotted. Crude peptocoagulase was nondialyzable, precipitated with (NH4)2SO4 at 40% saturation, somewhat resistant to heating at both neutral and acid pH, and chloroform insoluble. Culture filtrate did not contain proteolytic activity with albumin and casein, as substrates and no esterase activity was detected with tosylarginine and benzoylarginine methyl esters as substrates. The clotting reaction required peptocoagulase, prothrombin, and fibrinogen. The activation of prothrombin appeared to involve a stoichiometric reaction with peptocoagulase, possibly by formation of a stable complex.

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Section: Resultsmentioning

confidence: 99%