Studies on the origin, processing and quality of crude drugs. II Pharmacological evaluation of the Chinese crude drug "Zhu" in experimental stomach ulcer. 2 Inhibitory effect of extract of Atractylodes lancea on gastric secretion.

Nogami, Mari; Moriura, Toshitsugu; Kubo, Michinori; Tani, Tohru

doi:10.1248/cpb.34.3854

Cited by 41 publications

(27 citation statements)

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“…Inhibition of iNOS expression in murine macrophages may be another possible mechanism of nonsteroidal anti-inflammatory drugs. 17) It has been reported that families of Atractylodes prevent stomach damage through an anti-ulcer effect and an inhibitory action on gastric secretion, [18][19][20] and Atractylodes japonica consists of atractylon, hydroxyatractylon, 5aH,10b-selina-4(14),7(11)-diene-8-one. 6) Of these, atractylon, the major active constituent of Atractylodes japonica extracts, was reported to inhibit Na ϩ ,K ϩ -ATPase activity, 5) and this Na ϩ transport is closely implicated with PGE 2 production.…”

Section: Discussionmentioning

confidence: 99%

Atractylodes japonica Suppresses Lipopolysaccharide-Stimulated Expressions of Inducible Nitric Oxide Synthase and Cyclooxygenase-2 in RAW 264.7 Macrophages

Jang

Shin

Kim

et al. 2004

Biological & Pharmaceutical Bulletin

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Lipopolysaccharide (LPS) initiates a number of major cellular responses which play a vital role in the pathogenesis of inflammatory responses including activation of inflammatory cells and production of cytokines and other mediators. Of these, nitric oxide (NO) is endogenously generated from Larginine by nitric oxide synthase (NOS) and plays an important role in the regulation of many physiological processes. 1)Several isoforms of NOS exist and fall into three major classes: inducible NOS (iNOS), endothelial NOS (eNOS), and neuronal NOS (nNOS). NO synthesized by iNOS has been implicated as a mediator of rheumatoid arthritis and other autoimmune diseases. 2)In addition, prostaglandins (PGs) are key inflammatory mediators that are converted from arachidonic acid by cyclooxygenase. There are two isoforms of cyclooxygenase: cyclooxygenase-1 (COX-1) and cyclooxygenase-2 (COX-2). While COX-1 is a constitutively expressed form in normal physiologic functions, COX-2 is expressed only in response to inflammatory signals such as cytokines and bacterial endotoxin LPS. COX-2 produces large amounts of PGE 2 that induce inflammatory reaction. 3,4)Atractylodes japonica has traditionally been used for the treatment of water retention in the body. Administration of the aqueous extract of Atractylodes japonica causes diuresis in humans, and its alcohol extract also shows a diuretic effect in mice. 5,6) Atractylodes japonica is known to be effective for the control of pain and treatment of arthritis. It was reported that the family of Atractylodes possesses anti-inflammatory activity 7,8) and modulates the intestinal immune system. 9)However, the mechanism of the anti-inflammatory action of Atractylodes japonica has not been clarified yet. In the present study, the effect of Atractylodes japonica against LPS-stimulated expressions of COX-2 and iNOS in murine RAW 264.7 macrophages was investigated using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, reverse transcription-polymerase chain reaction (RT-PCR), PGE 2 immunoassay, and NO detection. MATERIALS AND METHODSCell Culture Murine macrophage RAW 264.7 cells were purchased from the Korean Cell Line Bank (KCLB, Seoul, Korea). Cells were cultured in Dulbecco's Modified Eagle Medium (DMEM) (Gibco BRL, Grand Island, NY, U.S.A.) supplemented with 10% heat-inactivated fetal bovine serum (FBS) (Gibco BRL) at 37°C in 5% CO 2 -95% O 2 in a humidified cell incubator. To obtain the water extract of Atractylodes japonica, 200 g of Atractylodes japonica was added to distilled water, and extraction was performed by heating at 80°C, concentrating with a rotary evaporator, and lyophilization. The resulting powder, weighing 30 g, was dissolved in saline.RNA Isolation and RT-PCR To identify the expressions of iNOS and COX-2 mRNAs, RT-PCR was performed. Total RNA was isolated from RAW 264.7 cells using RNAzol TM B (TEL-TEST, Friendswood, TX, U.S.A.). Two micrograms of RNA and 2 ml of random hexamers (Promega, Madison, WI, U.S.A.) were added together, and the mixture was he...

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Section: Discussionmentioning

confidence: 99%

Atractylodes japonica Suppresses Lipopolysaccharide-Stimulated Expressions of Inducible Nitric Oxide Synthase and Cyclooxygenase-2 in RAW 264.7 Macrophages

Jang

Shin

Kim

et al. 2004

Biological & Pharmaceutical Bulletin

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“…This is also clear from reports of the medicinal plants shakuyaku, sojutsu, and bukuryo in Toki-shakuyaku-san having anti-ulcer activity. 16,17) Pigmentation is thought to be an adverse reaction to an iron preparation which is generated by hemochromatosis. 18) In the Toki-shakuyaku-san group, there was no pigmentation in the tail, suggesting that the drug is involved in inhibiting pigmentation.…”

Section: Discussionmentioning

confidence: 99%

A Comparative Study of Laboratory Parameters and Symptoms Effected by Toki-shakuyaku-san and an Iron Preparation in Rats with Iron-Deficiency Anemia

Onodera

Matsushita

Akase

et al. 2004

Biological & Pharmaceutical Bulletin

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Kampo medicine (Sino-Japanese traditional herbal medicine) is widely adopted in contemporary medicine, and there are increasing evaluations of its efficacy and safety. Toki-shakuyaku-san is composed of 6 varieties of medicinal plants: toki, senkyu, shakuyaku, sojutsu, takusha, and bukuryo, and because it is efficacious due to its smooth muscle relaxation, facilitating hemopoiesis and adequate water level control in the body, etc., 1) it has long been considered effective in improving symptoms in women under a hypofunctioning condition.2) In the gynecologic and obstetric area, Tokishakuyaku-san has been used because it improves infertility, dysmenorrhea, and toxemia of pregnancy, and has a fetusstabilizing action, in addition to improving anemia.3,4) Moreover, it is a safe drug, with no teratogenicity having been observed in animal studies. 5)We have been focusing on the anemia-ameliorating effect of the Kampo medicine Toki-shakuyaku-san for some time, and based on a retrospective study comparing this and an iron preparation in patients with iron-deficiency anemia we have reported favorable results in terms of efficacy, safety, and economy. 6) In addition, when we conducted a prospective comparative study of the therapeutic effect of Toki-shakuyakusan and an iron preparation in iron-deficiency anemia in a common gynecologic disease, uterine myoma, we reported that the drug caused no significant improvement in the laboratory parameters, but marked resolution was noted of the symptoms and signs of anemia, menorrhagia, dysmenorrhea and the climacteric symptoms. On the other hand, the iron preparation improved the laboratory parameters, however, gastrointestinal side effects (heartburn, nausea, vomiting, diarrhea, constipation and abdominal distention) were encountered in more than 80% of patients receiving the iron preparation.7) There has also been a clinical report of an ameliorating effect on iron-deficiency anemia during pregnancy.8) Nevertheless, the pharmacological actions of Toki-shakuyaku-san against iron-deficiency anemia patients remain unknown.To closely examine the drug's effect of on the laboratory parameters and gastrointestinal system of iron-deficiency anemia, we prepared anemic rats, divided them into 4 groups, an untreated group, a group treated with Toki-shakuyaku-san (Toki-shakuyaku-san group), a group treated with an iron preparation (iron-preparation group), and a group treated with Toki-shakuyaku-san plus an iron preparation (Tokishakuyaku-sanϩiron-preparation group); we than assessed the results of hematology, blood biochemistry, erythrocyte morphology, organ weight, and fecal number, wet weight, water content, and iron content. MATERIALS AND METHODS AnimalsMale Wistar rats, 7 weeks old, were purchased from Sankyo Lab Service, Co., Inc., and after a 1-week preliminary period, the experiment was begun. The animal care conditions were a room temperature of 23Ϯ1°C, humidity of 55Ϯ5%, and lights-on at 8 a.m. and lights-off at 8 p.m. All animals were treated in accordance with the Guidelines for A...

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“…DC (Ouyang et al, 2007;Chen et al, 2007). These compounds including the rhizome extract have been shown to possess several pharmacological activities including anti-inflammatory (Resch et al, 2001), anti-angiogenic (Tsuneki et al, 2005), antimicrobial (Wang et al, 2009;Chen et al, 2012), antihypertensive , anti-ulcer and inhibitory activities on gastric secretion (Kubo et al, 1983;Nogami et al, 1986), as well as activity on nervous system (Nojima et al, 1992). Results of our previous studies demonstrated promising anti-CCA activity of the ethanolic extract of A. lancea (Thunb.)…”

Section: Introductionmentioning

confidence: 93%

Anticancer Activity of Atractylodes lancea (Thunb.) DC in a Hamster Model and Application of PET-CT for Early Detection and Monitoring Progression of Cholangiocarcinoma

Plengsuriyakarn¹,

Matsuda²,

Karbwang³

et al. 2015

Asian Pacific Journal of Cancer Prevention

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Studies on the origin, processing and quality of crude drugs. II Pharmacological evaluation of the Chinese crude drug "Zhu" in experimental stomach ulcer. 2 Inhibitory effect of extract of Atractylodes lancea on gastric secretion.

Cited by 41 publications

References 0 publications

Atractylodes japonica Suppresses Lipopolysaccharide-Stimulated Expressions of Inducible Nitric Oxide Synthase and Cyclooxygenase-2 in RAW 264.7 Macrophages

Atractylodes japonica Suppresses Lipopolysaccharide-Stimulated Expressions of Inducible Nitric Oxide Synthase and Cyclooxygenase-2 in RAW 264.7 Macrophages

A Comparative Study of Laboratory Parameters and Symptoms Effected by Toki-shakuyaku-san and an Iron Preparation in Rats with Iron-Deficiency Anemia

Anticancer Activity of Atractylodes lancea (Thunb.) DC in a Hamster Model and Application of PET-CT for Early Detection and Monitoring Progression of Cholangiocarcinoma

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