Studies on the partial characterization of extracted glycosaminoglycans from fish waste and its potentiality in modulating obesity through in-vitro and in-vivo

Geetha, V.; Das, Moumita; Zarei, Mahsa; VP, Mayookha; Harohally, Nanishankar V.; Kumar, G. Suresh

doi:10.1007/s10719-022-10077-5

Cited by 9 publications

(13 citation statements)

References 65 publications

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“…Fecal weight was increased in the e-GAGs treated groups compared to the HFD groups, this may be due to the fat content in fecal matter, our previous results have shown that the e-GAGs had bile acid binding property, which may have reduced the fat absorption and eliminated fat through the fecal matter, (10). Fatty acids elimination in the fecal matter showed that the HFD group had reduced SFA elimination, which was increased (p<0.05) with the GFD1&2 treated groups in dose dependent manner, these results agreed with the studies who have shown similar results in their animal experiments (20).…”

Section: Discussionmentioning

confidence: 80%

“…Our previous studies showed that e-GAGs from mackerel fish waste had health beneficial effects as an anti-obesity molecule by in-vitro and in-vivo mechanisms (10). To further elucidate probable pathways by which it can reduce obesity by controlling the lipid metabolism was studied in the present work.…”

Section: Discussionmentioning

confidence: 97%

“…The tissues were processed for the extraction of GAGs by chemical and enzymatic methods, hereafter referred to as extracted GAGs (e-GAGs). e-GAGs compositional analysis and partial structural predictions were done by techniques like electrophoresis, FTIR, SEM, 1 H NMR with our previous studies (10).…”

Section: Methodsmentioning

confidence: 99%

“…1. The experiment was for 120 days, following animal sacrifice, blood was drawn to separate the plasma, and the harvested organs were weighed and kept at -80°C until further processes (10).…”

Section: Animal Groupingmentioning

confidence: 99%

“…Our previous published work showed the partial characterization of e-GAGs (extracted glycosaminoglycans) from mackerel fish waste; its lipid lowering effects were studied in-vitro and in-vivo (10). However, there is not enough data available to compare the therapeutic potentials of fecal fat and fatty acid studies on the functional aspects of controlling lipid metabolism.…”

Section: Introductionmentioning

confidence: 99%

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Glycosaminoglycans from fish waste alleviate high fat diet induced obesity via modulating plasma biomarkers and fatty acid profile of obese C57BL/6 mice

Geetha¹,

G.²

2023

Biomedicine

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Introduction and Aim: Glycosaminoglycans are sulphated polysaccharides which shows various biological activities including anti-obesity, anti-inflammation etc., Extracted glycosaminoglycans (e-GAGs) from mackerel fish waste was studied for their potential as an anti-obesity molecule by in-vivo mechanisms. An attempt was made to study their beneficial effects in regulating plasma biomarkers and fatty acids of liver and fecal matter. Materials and Methods: In-vivo studies were conducted on male C57BL/6 mice (20-22g, 6-8 weeks of age). The animal studies had the IAEC (150/2019) clearance from CSIR-CFTRI, Mysuru. Animals were fed with a high fat diet and the e-GAGs treatment was at 0.4 and 0.8% through diet. Plasma analysis was done with commercially available kits; fatty acids were analyzed by gas chromatography as fatty acid methyl esters. Histopathology was carried out with H&E staining. Results: Plasma biomarkers like SGOT, SGPT, ALP, creatinine and LDH were reduced in the treatment groups in dose dependent manner compared to high fat fed groups. SFA in the liver was highest in the high fat fed group (54.25%), ameliorated to 50.25 and 48.35% with the e-GAGs fed at 0.4 and 0.8% through diet respectively. Fecal fatty acid composition showed the higher (p<0.05) elimination of SFA in the treatment groups. Tissue histopathology of spleen, kidney and pancreas showed the highest damage in the high fat fed group and normalized with the e-GAGs treatment. Conclusion: e-GAGs from fish waste could be beneficial in modulating obesity via modulating fatty acid composition and tissue protection from the obesity induced damage and benefit as a potent anti-obesity molecule.

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Section: Discussionmentioning

confidence: 80%

Section: Discussionmentioning

confidence: 97%

Section: Methodsmentioning

confidence: 99%

Section: Animal Groupingmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Glycosaminoglycans from fish waste alleviate high fat diet induced obesity via modulating plasma biomarkers and fatty acid profile of obese C57BL/6 mice

Geetha¹,

G.²

2023

Biomedicine

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Glycosaminoglycan dermatan sulfate supplementation decreases diet‐induced obesity and metabolic dysfunction in mice

Stojnić,

Galmés,

Serrano

et al. 2023

BioFactors

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Glycosaminoglycans are complex carbohydrates used as nutraceuticals for diverse applications. We studied the potential of the glycosaminoglycan dermatan sulfate (DS) to counteract the development of diet‐induced obesity (DIO) using obesity‐prone mice fed a high‐fat diet (HFD) as a model. Oral DS supplementation protected the animals against HFD‐induced increases in whole‐body adiposity, visceral fat mass, adipocyte size, blood glucose levels, insulin resistance, and pro‐inflammatory lipids levels in brown adipose tissue (BAT) and the liver, where it largely counteracted the HFD‐induced changes in the nonpolar metabolome. Protection against DIO in the DS‐supplemented mice occurred despite higher energy intake and appeared to be associated with increased energy expenditure, higher uncoupling protein 1 expression in BAT, decreased BAT “whitening,” and an enhanced channeling of fuel substrates toward skeletal muscle. This work is the first preclinical study to examine the anti‐obesity activity of DS tested individually in vivo. The results support possible uses of DS as an active component in functional foods/supplements to manage obesity and associated metabolic diseases.

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Bioactive carbohydrate polymers from marine sources as potent nutraceuticals in modulating obesity: a review

Geetha,

Mayookha,

Das

et al. 2024

Food Sci Biotechnol

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Studies on the partial characterization of extracted glycosaminoglycans from fish waste and its potentiality in modulating obesity through in-vitro and in-vivo

Cited by 9 publications

References 65 publications

Glycosaminoglycans from fish waste alleviate high fat diet induced obesity via modulating plasma biomarkers and fatty acid profile of obese C57BL/6 mice

Glycosaminoglycans from fish waste alleviate high fat diet induced obesity via modulating plasma biomarkers and fatty acid profile of obese C57BL/6 mice

Glycosaminoglycan dermatan sulfate supplementation decreases diet‐induced obesity and metabolic dysfunction in mice

Bioactive carbohydrate polymers from marine sources as potent nutraceuticals in modulating obesity: a review

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