Studies on the pathogenesis of ischemic cell injury VII. Proton gradient and respiration of renal tissue cubes, renal mitochondrial and submitochondrial particles following ischemic cell injury

Mergner, Wolfgang J.; Marzella, Louis; Mergner, C.; Kahng, Myong Won; Smith, Mary W.; Trump, Benjamin F.

doi:10.1016/s0005-8165(77)80079-6

Cited by 10 publications

(1 citation statement)

References 13 publications

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“…But the system tests the status of the produc tion site of ATP and can help to establish a time relation ship between the morphological appearance of flocculent densities and the declining function of ATP synthesis of mitochondria, the purpose of our study. It could also be expanded to review the relationship of other functions such as the respiratory complexes [9] or the ability to establish a proton gradient [6], and to measure the activity of the oligomycin-inhibited ATPase [33][34][35].…”

Section: Discussionmentioning

confidence: 99%

Measurement of ATP Synthesis and Flocculent Matrix Densities in Mitochondria as a Function of ‘in vitro’ Ischemia in the Heart and Liver of Rats

Costa

Shute²,

Mergner³

1990

Pathobiology

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We intended to determine the levels of adenosine triphosphate (ATP) synthesis at the time when mitochondria ultrastructurally show flocculent densities in the matrix space. For this purpose, mitochondria were isolated from rat heart and rat liver after the tissues have been maintained under controlled ischemic conditions in vitro at 37° C for intervals of 15, 30, 45, 60, 120, 180, and 240 (heart) min. The isolated mitochondria were tested for new ATP synthesis by luciferin/luciferase luminescence in the presence of substrate and adenosine 5’-diphosphate (ADP). The luminescence peaks were standardized and related to an external measure by measuring absorbance of ATP at 259 nm where the extinction coefficient is 15,400. Mitochondrial yield was monitored by measuring succinate dehydrogenase activity in the first homogenate and in the final mitochondrial pellet. Alternatively, cytochrome oxidase activity was used and the protein in the mitochondrial pellet was also determined. We found that the yield of mitochondria was above 53–54% in both liver and heart at 2 h of ischemia. Longer intervals were accompanied by lower yields. The ability to synthesize new ATP declined at different time intervals in ischemia of the heart compared to the liver. After 30 min ischemia, the synthesis in heart mitochondria is 18 % of control, while the synthesis of liver mitochondria reaches 16% of control after 45 min of in vitro ischemia. Flocculent densities in heart mitochondria appeared at 45 min ischemia in vitro and in vivo, and at 60 min in liver mitochondria. We conclude that the decline of ATP synthesis is a significant early change in mitochondria and antedates the appearance of flocculent densities.

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Section: Discussionmentioning

confidence: 99%

Measurement of ATP Synthesis and Flocculent Matrix Densities in Mitochondria as a Function of ‘in vitro’ Ischemia in the Heart and Liver of Rats

Costa

Shute²,

Mergner³

1990

Pathobiology

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Renal Metabolism: Integrated Responses

Klahr

Hamm

Hammerman

et al. 1992

Comprehensive Physiology

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The sections in this article are: Methodological Considerations Coupling of Metabolism to Transport Oxidative vs. Glycolytic Metabolism in the Mammalian Kidney Stoichiometry of Transepithelial Na + Transport to QO 2 Transport as the Pacemaker of Respiration Control of Transport by Metabolism Metabolic Substrate Utilization by the Kidney Metabolic Heterogeneity of the Kidney Metabolism of Glucose Lactate Metabolism Pyruvate Metabolism Renal Lipid Metabolism Amino Acid Metabolism Citrate Metabolism Ketone Metabolism Synthetic Functions of the Kidney Gluconeogenesis Alanine Serine Renal Phospholipid Metabolism Phospholipid Composition of Kidney Metabolism of Specific Renal Phospholipids Metabolism of Membrane Proteins in Kidney Phosphorylation of Membrane Proteins ADP ‐Ribosylation

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Acute Renal Failure and Toxic Nephropathy

Humes

Nguyen

1987

Contemporary Nephrology

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Studies on the pathogenesis of ischemic cell injury VII. Proton gradient and respiration of renal tissue cubes, renal mitochondrial and submitochondrial particles following ischemic cell injury

Cited by 10 publications

References 13 publications

Measurement of ATP Synthesis and Flocculent Matrix Densities in Mitochondria as a Function of ‘in vitro’ Ischemia in the Heart and Liver of Rats

Measurement of ATP Synthesis and Flocculent Matrix Densities in Mitochondria as a Function of ‘in vitro’ Ischemia in the Heart and Liver of Rats

Renal Metabolism: Integrated Responses

Acute Renal Failure and Toxic Nephropathy

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Studies on the pathogenesis of ischemic cell injury VII. Proton gradient and respiration of renal tissue cubes, renal mitochondrial and submitochondrial particles following ischemic cell injury

Cited by 10 publications

References 13 publications

Measurement of ATP Synthesis and Flocculent Matrix Densities in Mitochondria as a Function of &lsquo;in vitro&rsquo; Ischemia in the Heart and Liver of Rats

Measurement of ATP Synthesis and Flocculent Matrix Densities in Mitochondria as a Function of &lsquo;in vitro&rsquo; Ischemia in the Heart and Liver of Rats

Renal Metabolism: Integrated Responses

Acute Renal Failure and Toxic Nephropathy

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Product

Resources

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Measurement of ATP Synthesis and Flocculent Matrix Densities in Mitochondria as a Function of ‘in vitro’ Ischemia in the Heart and Liver of Rats

Measurement of ATP Synthesis and Flocculent Matrix Densities in Mitochondria as a Function of ‘in vitro’ Ischemia in the Heart and Liver of Rats