Studies on the Phagocytic function of Urinary Leukocytes

Maeda, Satoshi; Deguchi, Takashi; Kanimoto, Yusuke; Kuriyama, Manabu; Kawada, Yukimichi; Nishiura, Tsuneo

doi:10.1016/s0022-5347(17)52131-x

Cited by 14 publications

(5 citation statements)

References 3 publications

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“…Our observations of effective phagocytosis in urine at both 200 and 485 mosM agree with the results of others (20,24). Suzuki and colleagues showed that there was only a onethird loss in phagocytic activity when PMN exposed to urine with an osmolality of less than 200 or greater than 550 mosM were removed from the urine for phagocytosis studies (25).…”

Section: Discussionsupporting

confidence: 91%

“…This supports the observations of Cox and Hinman on the antibacterial activity of the bladder wall (7). Other workers have reported that PMN contribute to the defense of the bladder in experimental infections (15) and that PMN in the urine of patients with urinary tract infections contain phagocytosed bacteria (20); these reports do not establish whether the phagocytosis observed takes place in or at the surface of the bladder wall or in the urine.…”

supporting

confidence: 65%

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Effect of pH and osmolality on in vitro phagocytosis and killing by neutrophils in urine

1993

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Phagocytosis and intracellular killing of two strains ofEscherichia coli and a Staphylococcus saprophyticus by polymorphonuclear neutrophils (PMN) in pooled sterile urine at three osmolalities (800, 485, and 200 mosMlkg of H20) between pHs 5 and 8 was investigated. Urine at 800 mosM virtually abolished phagocytosis of both E. coli strains, regardless of pH, and reduced the phagocytosis of S. saprophyticus to 30%; no killing of any organisms took place at this osmolality. On the other hand, phagocytosis was as good in urine as in Hanks balanced salt solution at both 485 and 200 mosM between pHs 6 and 8. Phagocytosis of all three strains was virtually abolished at pH 5. Killing of the strains by PMN was optimal between pHs 6.5 and 7.5 in urine at 485 mosM (being at least 90% of the control values in Hanks balanced salt solution), whereas at 200 mosM killing was reduced to 50 to 70%o of these values. Reduced killing of all three strains occurred at pH 8, whereas at pH 6 only S. saprophyticus was killed. Thus, the bactericidal activity of PMN in urine was more sensitive than 8

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Section: Discussionsupporting

confidence: 91%

supporting

confidence: 65%

Effect of pH and osmolality on in vitro phagocytosis and killing by neutrophils in urine

1993

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“…Stadler et al (34) and Gillon et al (11) have histologically indicated that PMN, together with locally produced opsonizing and agglutinating antibodies, participate in bacterial clearance from the bladder in P. mirabilis and E. coli UTI models using mice undergoing water-diuresis. Maeda et al (20) have shown by careful lightand electron-microscopic examinations of urine specimens from patients with UTI that PMN actually phagocytize bacteria with a high frequency. All these authors have concluded that the phagocytic activity of PMN constitutes a crucial defense mechanism in the bladder against bacterial infection.…”

Section: Discussionmentioning

confidence: 99%

“…However, some early studies documented that since the phagocytic function of PMN was suppressed in the urine by its extremes of osmolarity, pH, and urea concentration, PMN might not play an important role in the pathogenesis of UTI (6,7,23). Conversely, subsequent histological studies on experimental UTI models have found that bacteria that penetrated into the submucosa of the bladder wall were often phagocytosed by PMN drained through the lymphatics or veins, and have suggested that phagocytic killing by PMN does play an important role in the defense of the bladder against bacterial infection (8,11,20,26,34). Some analytical studies on the initial stages of host-parasite interaction in an experimental UTI mouse model with E. coli have also indicated that the accumulation of inflammatory cells in the kidneys and bladder tissue is a major defense mechanism against E. coli in the urinary tract (12,13,31,37).…”

mentioning

confidence: 99%

Interaction of Escherichia coli with polymorphonuclear leukocytes in pathogenesis of urinary tract infection in mice

Iwahi

Imada

1988

Infect Immun

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Two type 1 fimbria-producing strains of Escherichia coli, 31-B and K12W1-3, and two type 1 fimbriaedefective mutants derived from 31-B, BH5 and BH9, were compared for their capacity to induce vesical infection in mice undergoing water diuresis and to interact in vitro with murine peritoneal exudate polymorphonuclear leukocytes (PMN). Strains 31-B and BH5 caused rapid bacterial multiplication in the bladder wall after being inoculated intrabladderly; their log-phase cells grown at 37°C, in striking contrast to their stationary-phase or 17°C-grown cells, resisted phagocytic killing by PMN in the presence of normal murine serum. Strains K12W1-3 and BH9 failed to cause vesical infection, and their cells were always susceptible to the opsonophagocytic killing by PMN irrespective of the growth conditions. Nevertheless, the log-phase cells of the three isogenic strains, 31-B, BH5, and BH9, grown at 37°C gave almost the same chemiluminescent response patterns during incubation with PMN in normal serum. The phagocytic resistance in strains 31-B and BH5 was eliminated by briefly treating bacterial cells with EDTA. These results suggest that the two virulent strains may express an antiphagocytic activity during their growth in the bladder and continue to stimulate the oxidative metabolic burst of PMN without being ingested and killed, and that the antiphagocytic activity may be related to a bacterial surface component(s) that is removed by EDTA.

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“…It is becoming clear that in addition to facilitating the initiation of bacterial infections, adhesive fimbriae expressed by different pathogenic bacteria may modulate survival in the host by modifying the interaction of bacteria with various inflammatory cells, including neutrophils (30,39). Histological examination of kidney and bladder tissue in experimental monkey and mouse models indicates that accumulation of neutrophils in the kidney and bladder tissue is a major host defense mechanism against E. coli-induced UTI (13,24,28,38). The fact that large numbers of neutrophils are often found in the urine of patients with UTI is another indication of the active involvement of neutrophils in host defense in the urinary tract.…”

mentioning

confidence: 99%

The PapG tip adhesin of P fimbriae protects Escherichia coli from neutrophil bactericidal activity

et al. 1994

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Compared with Escherichia coli ORN103, a nonfimbriated K-12 strain, P-fimbriated E. coli ORN103/pPAP5 was found to interact poorly with human neutrophils and resist their bactericidal activity in vitro. PapG, the Gal alpha(1-->4)Gal binding moiety located at the distal end of the P fimbrial filament, appeared to be responsible for this effect because an isogenic PapG- mutant, E. coli ORN103/pPAP24, exhibited binding interactions with neutrophils that were similar to nonfimbriated E. coli ORN103. Although no direct evidence is available, the poor adherence mediated by PapG could be related to its electrostatic properties because the isolated PapG protein had a pI of 5.2, which indicated that in the physiological pH range it possessed a net negative charge. Antibodies against PapG overcame the protective effect of PapG and markedly enhanced the interactions of P-fimbriated E. coli with neutrophils resulting in bacterial killing. When a P-fimbriated clinical E. coli strain or its isogenic PapG- derivative was injected into the peritoneal cavities of mice, a similar number of neutrophils was recruited to the site of injection. After 2 h, the number of P-fimbriated E. coli organisms that survived the neutrophil influx in the mouse peritoneum was approximately four times more than the number of surviving PapG- bacteria. This result demonstrates that the PapG protein, which is strategically located at the distal region of the P-fibrillum structure, protects E. coli from the bactericidal action of neutrophils.

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Studies on the Phagocytic function of Urinary Leukocytes

Cited by 14 publications

References 3 publications

Effect of pH and osmolality on in vitro phagocytosis and killing by neutrophils in urine

Effect of pH and osmolality on in vitro phagocytosis and killing by neutrophils in urine

Interaction of Escherichia coli with polymorphonuclear leukocytes in pathogenesis of urinary tract infection in mice

The PapG tip adhesin of P fimbriae protects Escherichia coli from neutrophil bactericidal activity

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