Studies on the Physical Characteristics of the Radio-Frequency Atmospheric-Pressure Glow Discharge Plasmas for the Genome Mutation of Methylosinus trichosporium

Influences of the cold atmospheric plasma jet treatment on the properties of the demineralized dentin surfaces

“…In this study, the RF-APGD plasma jet is used for the treatment of the dentin samples, as shown in figure 1. The detailed descriptions on the experimental setup can be referred to [21]. The operating parameters for producing the uniform, low temperature plasma jet are as follows: the driving frequency of the power supply f=13.56 MHz, the RF power input P in =15 W, helium flow rate Q=4 slpm.…”

“…However, the gas temperature in the plasma jet region was higher than the room temperature, e.g., 38°C in [20], which might lead to heat accumulation inside the samples during plasma treatment. In our previous studies, a radio-frequency, atmospheric-pressure glow discharge (RF-APGD) plasma jet using a water-cooled, bare-metallic electrode configuration has been developed for the genome mutation of micro-organisms [1,21]. Our studies showed that the RF-APGD was a uniform glow discharge with no filaments, and the gas temperatures were low and controllable by adjusting the operating conditions appropriately [21][22][23][24].…”

“…In our previous studies, a radio-frequency, atmospheric-pressure glow discharge (RF-APGD) plasma jet using a water-cooled, bare-metallic electrode configuration has been developed for the genome mutation of micro-organisms [1,21]. Our studies showed that the RF-APGD was a uniform glow discharge with no filaments, and the gas temperatures were low and controllable by adjusting the operating conditions appropriately [21][22][23][24]. In this study, by adjusting the RF power input and the temperature of the plasma working gas at the inlet of the plasma generator, the gas temperature of the plasma jet can be controlled at a little bit lower level than the room temperature, which means that the heat accumulation inside the treated samples can be avoided during the plasma treatment process.…”

Spectral characteristics of cotton seeds treated by a dielectric barrier discharge plasma

“…Initially building on advances in plasma-assisted material fabrication 6–9, 10 , exhaust gas purification 11 , and wastewater treatment 12, 13 , significant progress has been made in the use of plasmas to selectively induce apoptosis in cancer cells 14–16 , to eliminate bacterial biofilms on living surfaces and promote wound healing 17, 18 , for pathogenic microorganism inactivation and removal from solid and liquid media 19, 20 , for mutation breeding 21 and for agricultural production 4, 22, 23 . The unique advantage of using plasmas over other methods of biological stimulation stems from its multi-modal activity, specifically the simultaneous production of chemical species, including reactive oxygen species (ROS) and reactive nitrogen species (RNS) noted for their catalytic activity and biological significance, highly-energetic electrons, electromagnetic radiation and thermal effects, which individually and synergistically affect the treated target 24, 25 .…”

Quantitative evaluation of DNA damage and mutation rate by atmospheric and room-temperature plasma (ARTP) and conventional mutagenesis

“…Recently, a novel mutagenic breeding method, atmospheric and room temperature plasma (ARTP), has attracted general interest because its features include rapid mutation, highly diverse mutants, and simple and safe operation (Li et al 2012;Zhang et al 2014a, b). ARTP has been used for phenotype improvement (e.g., in productivity, toxicity tolerance, growth rate) in more than 40 kinds of microorganisms (Fang et al 2013;Hua et al 2010;Jiang et al 2014;Wang et al 2010;Zhang et al 2014a).…”